In vivo evaluation of resorbable bone graft substitutes in a rabbit tibial defect model

Calcium phosphate ceramics, of a similar composition to that of mineral bone, and which possess the properties of bioactivity and osteoconductivity, have been widely used as substitutes for bone graft in orthopedic, plastic and craniofacial surgeries. A synthetic β-tricalcium phosphate, Osteocera™, a recently developed bone graft substitute, has been used in this study. To evaluate the affinity and efficacy of Osteocera™ as bone defect implant, we used a New Zealand white rabbit femur defect model to test the osteoconductivity of this new bone substitute. Alternative commercially available bone substitutes, Triosite® and ProOsteon500, were used as the control materials. These three bone substitutes show good biocompatibility, and no abnormal inflammation either infection was seen at the implantation sites. In the histological and histomorphometric images, newly formed bone grew into the peripheral pores in the bone substitutes. After six months implantation, the volume of bone formation was found to be 20.5 ± 5.2%, 29.8 ± 6.5% and 75.5 ± 4.9% of the potential total cavity offered by ProOsteon500, Triosite® and Osteocera™, respectively. The newly formed bone area within the femur defect section for Osteocera™ was significantly larger than ProOsteon500 and Triosite®. We concluded that Osteocera™ shows better bioresorbability, biocompatibility and osteoconductivity in the rabbit femur defect model.

Download Full-text

Zirconium modified calcium‐silicate‐based nanoceramics: An in vivo evaluation in a rabbit tibial defect model

International Journal of Applied Ceramic Technology ◽

10.1111/ijac.13076 ◽

2018 ◽

Vol 16 (2) ◽

pp. 431-437 ◽

Cited By ~ 4

Author(s):

Ali Doostmohammadi ◽

Zahra Karimzadeh Esfahani ◽

Abdolreza Ardeshirylajimi ◽

Zahra Rahmati Dehkordi

Keyword(s):

Calcium Silicate ◽

Defect Model ◽

In Vivo Evaluation ◽

Tibial Defect

Download Full-text

Synthesis and in vivo evaluation of a scaffold containing wollastonite/β‐TCP for bone repair in a rabbit tibial defect model

Journal of Biomedical Materials Research Part B Applied Biomaterials ◽

10.1002/jbm.b.34462 ◽

2019 ◽

Vol 108 (3) ◽

pp. 1107-1116 ◽

Cited By ~ 5

Author(s):

Willams T. Barbosa ◽

Katilayne V. Almeida ◽

Gabriel G. Lima ◽

Miguel A. Rodriguez ◽

Marcos V. Lia Fook ◽

...

Keyword(s):

Bone Repair ◽

Defect Model ◽

In Vivo Evaluation ◽

Tibial Defect

Download Full-text

Fabrication of hybrid scaffolds by polymer deposition system and its in-vivo evaluation with a rat tibial defect model

Tissue Engineering and Regenerative Medicine ◽

10.1007/s13770-014-0065-0 ◽

2014 ◽

Vol 11 (6) ◽

pp. 439-445 ◽

Cited By ~ 8

Author(s):

Min-Woo Sa ◽

Sung Eun Kim ◽

Young-Pil Yun ◽

Hae-Ryong Song ◽

Jong Young Kim

Keyword(s):

Defect Model ◽

In Vivo Evaluation ◽

Tibial Defect ◽

Polymer Deposition ◽

Hybrid Scaffolds

Download Full-text

β-TCP bone graft substitutes in a bilateral rabbit tibial defect model

Biomaterials ◽

10.1016/j.biomaterials.2007.09.035 ◽

2008 ◽

Vol 29 (3) ◽

pp. 266-271 ◽

Cited By ~ 131

Author(s):

William R. Walsh ◽

Frank Vizesi ◽

Dean Michael ◽

Jason Auld ◽

Andy Langdown ◽

...

Keyword(s):

Bone Graft ◽

Defect Model ◽

Tibial Defect ◽

Bone Graft Substitutes

Download Full-text

In Vivo Evaluation of 3D-Printed Polycaprolactone Scaffold Implantation Combined with β-TCP Powder for Alveolar Bone Augmentation in a Beagle Defect Model

Materials ◽

10.3390/ma11020238 ◽

2018 ◽

Vol 11 (2) ◽

pp. 238 ◽

Cited By ~ 33

Author(s):

Su Park ◽

Hyo-Jung Lee ◽

Keun-Suh Kim ◽

Sang Lee ◽

Jung-Tae Lee ◽

...

Keyword(s):

Alveolar Bone ◽

Bone Augmentation ◽

Defect Model ◽

In Vivo Evaluation ◽

3D Printed

Download Full-text

In vivo evaluation of interactions between biphasic calcium phosphate (BCP)-niobium pentoxide (Nb2O5) nanocomposite and tissues using a rat critical-size calvarial defect model

Journal of Materials Science Materials in Medicine ◽

10.1007/s10856-020-06414-5 ◽

2020 ◽

Vol 31 (8) ◽

Author(s):

Helio de Jesus Kiyochi Junior ◽

Aline Gabriela Candido ◽

Taiana Gabriela Moretti Bonadio ◽

José Adauto da Cruz ◽

Mauro Luciano Baesso ◽

...

Keyword(s):

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Critical Size ◽

Niobium Pentoxide ◽

Calvarial Defect ◽

Defect Model ◽

In Vivo Evaluation

Download Full-text

In Vitro and In Vivo Evaluation Of Calcium Phosphate Bone Graft Substitutes

Materials Science Forum ◽

10.4028/www.scientific.net/msf.654-656.2065 ◽

2010 ◽

Vol 654-656 ◽

pp. 2065-2070

Author(s):

Ho Yeon Song ◽

Young Hee Kim ◽

Jyoti M. Anirban ◽

In Seon Byun ◽

Kyung A Kwak ◽

...

Keyword(s):

Calcium Phosphate ◽

Bone Formation ◽

Bone Graft ◽

Cellular Proliferation ◽

New Bone Formation ◽

Hydroxy Apatite ◽

Defect Sites ◽

Bone Graft Substitutes

Calcium phosphate ceramics such as hydroxy apatite (HA), β-tricalcium phosphate (β-TCP) and bicalcium phosphate (BCP) have been used as a bone graft biomaterial because of their good biocompatibility and similarity of chemical composition to natural bones. To increase the mechanical and osteoconductive properties, the granules and spongy type porous bone graft substitutes were prepared by fibrous monolithic process and polyurethane foam replica methods, respectively. The pore sizes obtained using these approaches ranged between 100-600 µm. The cytotoxicity, cellular proliferation, differentiation and ECM deposition on the bone graft substitutes were observed by SEM and confocal microscopy. Moreover, the scaffolds were implanted in the rabbit femur. New bone formation and biodegradation of bone graft were observed through follow-up X-ray, micro-CT analysis and histological findings. After several months (2, 3, 6, 12 and 24 months) of implantation, new bone formation and ingrowths were observed in defect sites of the animal by CaP ceramics and 2 to 3 times higher bone ingrowths were confirmed than that of the normal trabecular bones in terms of total bone volume (BV).

Download Full-text