The osteogenic differentiation of rat muscle-derived stem cells in vivo within in situ-forming chitosan scaffolds

Biomaterials ◽  
2008 ◽  
Vol 29 (33) ◽  
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Jung Hwa Lee ◽  
Hyun Hee Ahn ◽  
Ju Young Lee ◽  
Gilson Khang ◽  
...  
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Sung Won Kim ◽  
Doo Yeon Kwon ◽  
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Vol 15 (7) ◽  
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Hyun Hee Ahn ◽  
Kyung Sook Kim ◽  
Jung Hwa Lee ◽  
Ju Young Lee ◽  
Byung Soo Kim ◽  
...  

Materials ◽  
2013 ◽  
Vol 6 (7) ◽  
pp. 2978-2988 ◽  
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Da Kim ◽  
Yoon Kim ◽  
Hai Lee ◽  
Shin Moon ◽  
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Moon Suk Kim ◽  
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Maurycy Jankowski ◽  
Mariusz Kaczmarek ◽  
Grzegorz Wąsiatycz ◽  
Claudia Dompe ◽  
Paul Mozdziak ◽  
...  

Next-generation sequencing (RNAseq) analysis of gene expression changes during the long-term in vitro culture and osteogenic differentiation of ASCs remains to be important, as the analysis provides important clues toward employing stem cells as a therapeutic intervention. In this study, the cells were isolated from adipose tissue obtained during routine surgical procedures and subjected to 14-day in vitro culture and differentiation. The mRNA transcript levels were evaluated using the Illumina platform, resulting in the detection of 19,856 gene transcripts. The most differentially expressed genes (fold change >|2|, adjusted p value < 0.05), between day 1, day 14 and differentiated cell cultures were extracted and subjected to bioinformatical analysis based on the R programming language. The results of this study provide molecular insight into the processes that occur during long-term in vitro culture and osteogenic differentiation of ASCs, allowing the re-evaluation of the roles of some genes in MSC progression towards a range of lineages. The results improve the knowledge of the molecular mechanisms associated with long-term in vitro culture and differentiation of ASCs, as well as providing a point of reference for potential in vivo and clinical studies regarding these cells’ application in regenerative medicine.


2017 ◽  
Vol 75 ◽  
pp. 279-285 ◽  
Author(s):  
Maria José Moura ◽  
João Brochado ◽  
Maria Helena Gil ◽  
Maria Margarida Figueiredo

2007 ◽  
Vol 25 (6) ◽  
pp. 1347-1354 ◽  
Author(s):  
Heiko Kranz ◽  
Erol Yilmaz ◽  
Gayle A. Brazeau ◽  
Roland Bodmeier

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