Retention of hemicellulose during delignification of oil palm empty fruit bunch (EFB) fiber with peracetic acid and alkaline peroxide

Abstract Enzymatic hydrolysis of oil palm empty fruit bunch (OPEFB) that has been pretreated by modified pretreatment has been investigated in this study. The OPEFB used was pretreated by using sequential peracetic acid – alkaline peroxide solution. As the modification method, the assistance of pretreatment by ultrasound was conducted, in order to increase the enzyme accessibility. Therefore, it enhances the production of reducing sugar on the hydrolysis process. Prior to hydrolysis process, OPEFB was initially treated by using peracetic acid solution, comprise of CH3COOH (> 99%) and H2O2 (30% w/w), assisted by ultrasound for 3 hours at 35oC. Afterwards, OPEFB was treated by using alkaline peroxide solution, comprise of NaOH (40% w/w) and H2O2 (35% w/w), assisted by ultrasound for 10 hours at 35oC. OPEFB that has been pretreated was then subjected to enzymatic hydrolysis process using cellulase enzyme, in order to convert cellulose content into reducing sugar. Enzymatic hydrolysis was carried out at 50oC in a shaker incubator with 150 rpm for 48 hours. In this study, the effect of different enzyme concentration and hydrolysis time towards the sugar concentration in modified-pretreated OPEFB was observed and analyzed. Three different concentrations of enzyme were used, including 1.25, 2.5, and 5 g/L, and reducing sugar concentrations were analyzed at 30 and 45 minutes, and 1, 2, 4, 6, 24, 30, and 48 hours. Based on results, enzyme concentration has a significant effect to the production of reducing sugar. The reducing sugar concentrations obtained at the end of the hydrolysis process were 8.48, 11.06, 19.16 g/L, at the enzyme concentrations of 1.25, 2.5, and 5 g/L, respectively. At any hydrolysis time, the highest sugar concentration has been achieved on the highest enzyme concentration of 5 g/L. Moreover, the effective hydrolysis time were achieved at 6 hours, at all concentration of enzyme, since the production of reducing sugar were insignificant after 6 hours. This study showed an increase in reducing sugar production by 8.25% in the hydrolysis process using OPEFB pretreated by modified pretreatment compared to the non-modified pretreatment.

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Alkaline peroxide pulping of oil palm empty fruit bunch by variation of chemical strength

10.1063/1.4757477 ◽

2012 ◽

Cited By ~ 4

Author(s):

Yunita Megasari Dermawan ◽

Arniza Ghazali ◽

Wan Rosli Wan Daud ◽

Mohd Azli Khairil Mat Lazin

Keyword(s):

Oil Palm ◽

Empty Fruit Bunch ◽

Alkaline Peroxide

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Glucose and Xylose Productions From Oil Palm Empty Fruit Bunch by Hydrolysis With Enzyme and Acid Using Response Surface Methodology.

10.21203/rs.3.rs-768878/v1 ◽

2021 ◽

Author(s):

Santat Sinjaroonsak ◽

Aran H-Kittikun ◽

Thanongsak Chaiyaso ◽

Wasana Suyotha

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Oil Palm ◽

Empty Fruit Bunch ◽

Solid Ratio ◽

Alkaline Peroxide ◽

Hydrolysis Process ◽

Cellulosic Waste ◽

Palm Oil Mill ◽

Hcl Concentration

Abstract Oil palm empty fruit bunch (EFB) is a major cellulosic waste from a palm oil mill. The use of EFB for bioconversion to fuel and valuable products is possible because this biomass is a cheap, renewable and abundantly available. This study was aimed to produce sugars from the alkaline peroxide pretreated EFB (APEFB) by hydrolysis with a commercial enzyme (iKnowzyme acid 2XL cellulase) in comparison with hydrochloric acid. Response surface methodology (RSM) was applied to improve the hydrolysis process. For an enzymatic hydrolysis, the optimum enzyme dose of 40 U/g APEFB and the liquid to solid ratio of 10 ml/g APEFB were investigated at 150 rpm and 50°C for 120 h. After saccharification, glucose and xylose obtained were 65.71 g/l (0.66 g/g APEFB) and 2.14 g/l (0.02 g/g APEFB), respectively. Many acids (acetic, formic, hydrochloric (HCl), nitric, orthophosphoric and sulfuric acids) were used to hydrolyze APEFB. The result showed that HCl was the best acid to produce glucose and xylose from APEFB with low furfural and hydroxymethylfurfural productions. The optimum HCl concentration and temperature for APEFB saccharification were 5.85% (w/v) acid at 114°C for 90 min. The glucose, xylose, furfural, and hydroxymethylfurfural obtained under these conditions were 10.70 g/l (0.11 g/g APEFB), 15.30 g/l (0.15 g/g APEFB), 2.34 g/l (0.02 g/g APEFB) and 0.67 g/l (0.007 g/g APEFB), respectively.

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