Synthesis and biological effects of small molecule enhancers for improved recombinant protein production in plant cell cultures

2020 ◽  
Vol 94 ◽  
pp. 103452 ◽  
Author(s):  
Bárbara A. Rebelo ◽  
Rita B. Santos ◽  
Osvaldo S. Ascenso ◽  
Ana Cláudia Nogueira ◽  
Diana Lousa ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Plant Cell ◽  
Cell Cultures ◽  
Small Molecule ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Biological Effects ◽  
Plant Cell Cultures ◽  
Small Molecule Enhancers

Continuous bleed recycling significantly increases recombinant protein production yield in perfusion cell cultures

2021 ◽  
Vol 169 ◽  
pp. 107966
Author(s):  
Jean-Marc Bielser ◽  
Mathieu Aeby ◽  
Stefania Caso ◽  
Anaïs Roulet ◽  
Hervé Broly ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Cell Cultures ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Production Yield

Ribosome-Inactivating Protein Production from Trichosanthes kirilowii Plant Cell Cultures

1994 ◽  
Vol 10 (4) ◽  
pp. 345-352 ◽  
Author(s):  
John E. Thorup ◽  
Karen A. McDonald ◽  
Alan P. Jackman ◽  
Nishant Bhatia ◽  
Abhaya M. Dandekar
Keyword(s):  
Plant Cell ◽  
Cell Cultures ◽  
Protein Production ◽  
Plant Cell Cultures ◽  
Ribosome Inactivating Protein ◽  
Trichosanthes Kirilowii

scholarly journals Effect of encephalomyocarditis virus 3'Untranslated region on GFP transient expression: implications in recombinant protein production

2020 ◽  
Vol 19 (6) ◽  
pp. 542-554
Author(s):  
Marzieh Marzbany ◽  
◽  
Mahsa Rasekhian ◽  
Keyword(s):  
Recombinant Protein ◽  
Mammalian Cell ◽  
Cell Cultures ◽  
Mrna Stability ◽  
Untranslated Region ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Encephalomyocarditis Virus ◽  
Dependent Manner ◽  
Mammalian Cell Cultures

The enrichment of therapeutic protein production yield in mammalian cell cultures by modulating mRNA stability is a fairly new strategy in biotechnological applications. Here, we describe the application of 3′-untranslated region (3′UTR) from RNA viral genome to modulate mRNA stability. The data obtained showed that the use of the 3 'UTR sequence of the encephalomyocarditis virus (EMCV 3'UTR) downstream of the target gene was not able to significantly modulate the free energy density indicators of the RNA. However, the sequence influenced the stability of the mRNA (and, therefore, the amount of protein production) in a cell type and time-dependent manner, indicating a central role of mRNA-stabilizing binding sites/cellular factors in this process. Our data might be of interest for the biotechnology community to improve recombinant protein production in mammalian cell cultures and RNA-based therapy/vaccination approaches.

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