scholarly journals Epigenetic changes in inflammatory genes and the protective effect of cooked rhubarb on pancreatic tissue of rats with chronic alcohol exposure

2022 ◽  
Vol 146 ◽  
pp. 112587
Author(s):  
Xianlin Zhao ◽  
Shifeng Zhu ◽  
Juan Li ◽  
Dan Long ◽  
Meihua Wan ◽  
...  
Keyword(s):  
Protective Effect ◽  
Alcohol Exposure ◽  
Pancreatic Tissue ◽  
Epigenetic Changes ◽  
Chronic Alcohol ◽  
Inflammatory Genes ◽  
Chronic Alcohol Exposure

CB1R Promotes Chronic Alcohol-Induced Neuronal Necroptosis in Mice Prefrontal Cortex

2020 ◽  
Author(s):  
Lin Ye ◽  
Shuhao Li ◽  
Xiaochen Liu ◽  
Dingang Zhang ◽  
Liliang Li ◽  
...  
Keyword(s):  
Prefrontal Cortex ◽  
Protein Level ◽  
Cannabinoid Receptors ◽  
Alcohol Exposure ◽  
Global Burden ◽  
Dependent Manner ◽  
Chronic Alcohol ◽  
Inverse Agonists ◽  
Alcohol Vapor ◽  
Chronic Alcohol Exposure

Abstract Aims Alcohol abuse induces multiple neuropathology and causes global burden to human health. Prefrontal cortex (PFC) is one of the most susceptible regions to alcohol-induced neuropathology. However, precise mechanisms underlying these effects on PFC remain to be elucidated. Herein, we investigated whether RIP1/RIP3/MLKL-mediated necroptosis was involved in the alcohol-induced PFC injury, and explored the effect that cannabinoid receptors (CBRs) exerted on the neurotoxicity of alcohol. Methods In this study, dynamic development of neuronal necroptosis in the PFC region was monitored after 95% (v/v) alcohol vapor administration for 15 and 30 days, respectively. Selective CBRs agonists or inverse agonists were pretreated according to the experimental design. All the PFC tissues were isolated and further examined by biochemical and histopathological analyses. Results It was found that chronic alcohol exposure increased the protein level of MLKL and also the phosphorylated levels of RIP1, RIP3 and MLKL in a time-dependent manner, all of which indicated the activation of necroptosis signaling. Particularly, compared to astrocytes, neurons from the PFC showed more prototypical necrotic morphology in response to alcohol insults. In parallel, an increased protein level of CB1R was also found after 15 and 30 days alcohol exposure. Administration of specific inverse agonists of CB1R (AM251 and AM281), but not its agonists or CB2R modulators, significantly alleviated the RIP1/RIP3/MLKL-mediated neuronal necroptosis. Conclusion We reported the involvement of RIP1/RIP3/MLKL-mediated necroptosis in alcohol-induced PFC neurotoxicity, and identified CB1R as a critical regulator of neuronal necroptosis that enhanced our understanding of alcohol-induced neuropathology in the PFC.

scholarly journals Chronic alcohol exposure inhibits biotin uptake by pancreatic acinar cells: possible involvement of epigenetic mechanisms

2014 ◽  
Vol 307 (9) ◽  
pp. G941-G949 ◽  
Author(s):  
Padmanabhan Srinivasan ◽  
Rubina Kapadia ◽  
Arundhati Biswas ◽  
Hamid M. Said
Keyword(s):  
Transgenic Mice ◽  
Chronic Exposure ◽  
Methylation Status ◽  
Acinar Cells ◽  
Alcohol Exposure ◽  
Significant Inhibition ◽  
Pancreatic Acinar Cells ◽  
Chronic Alcohol ◽  
Wild Type ◽  
Chronic Alcohol Exposure

Chronic exposure to alcohol affects different physiological aspects of pancreatic acinar cells (PAC), but its effect on the uptake process of biotin is not known. We addressed this issue using mouse-derived pancreatic acinar 266-6 cells chronically exposed to alcohol and wild-type and transgenic mice (carrying the human SLC5A6 5′-promoter) fed alcohol chronically. First we established that biotin uptake by PAC is Na+ dependent and carrier mediated and involves sodium-dependent multivitamin transporter (SMVT). Chronic exposure of 266-6 cells to alcohol led to a significant inhibition in biotin uptake, expression of SMVT protein, and mRNA as well as in the activity of the SLC5A6 promoter. Similarly, chronic alcohol feeding of wild-type and transgenic mice carrying the SLC5A6 promoter led to a significant inhibition in biotin uptake by PAC, as well as in the expression of SMVT protein and mRNA and the activity of the SLC5A6 promoters expressed in the transgenic mice. We also found that chronic alcohol feeding of mice is associated with a significant increase in the methylation status of CpG islands predicted to be in the mouse Slc5a6 promoters and a decrease in the level of expression of transcription factor KLF-4, which plays an important role in regulating SLC5A6 promoter activity. These results demonstrate, for the first time, that chronic alcohol exposure negatively impacts biotin uptake in PAC and that this effect is exerted (at least in part) at the level of transcription of the SLC5A6 gene and may involve epigenetic/molecular mechanisms.

EFFECTS OF CHRONIC ALCOHOL EXPOSURE ON GROWTH AND NUTRITION IN RATS

1976 ◽  
Vol 273 (1 Work in Progr) ◽  
pp. 205-211 ◽  
Author(s):  
Jack Wang ◽  
Mark Marvin ◽  
Bruce Abel ◽  
Richard N. Pierson
Keyword(s):  
Alcohol Exposure ◽  
Chronic Alcohol ◽  
Growth And Nutrition ◽  
Chronic Alcohol Exposure

Chronic alcohol exposure induced gut microbiota dysbiosis and its correlations with neuropsychic behaviors and brain BDNF/Gabra1 changes in mice

BioFactors ◽  
2018 ◽  
Vol 45 (2) ◽  
pp. 187-199 ◽  
Author(s):  
Zheng Xu ◽  
Can Wang ◽  
Xiaoguang Dong ◽  
Tao Hu ◽  
Lingling Wang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Alcohol Exposure ◽  
Chronic Alcohol ◽  
Chronic Alcohol Exposure ◽  
Gut Microbiota Dysbiosis

scholarly journals Basic research Chronic alcohol exposure increases ganglia endogenous morphine levels

2010 ◽  
Vol 3 ◽  
pp. 316-320 ◽  
Author(s):  
Wei Zhu ◽  
Yiqing Li ◽  
Richard M. Kream ◽  
George B. Stefano
Keyword(s):  
Basic Research ◽  
Alcohol Exposure ◽  
Chronic Alcohol ◽  
Chronic Alcohol Exposure ◽  
Endogenous Morphine

scholarly journals Chronic alcohol exposure disrupts CB1regulation of GABAergic transmission in the rat basolateral amygdala

2016 ◽  
Vol 22 (3) ◽  
pp. 766-778 ◽  
Author(s):  
Florence P. Varodayan ◽  
Michal Bajo ◽  
Neeraj Soni ◽  
George Luu ◽  
Samuel G. Madamba ◽  
...  
Keyword(s):  
Basolateral Amygdala ◽  
Alcohol Exposure ◽  
Chronic Alcohol ◽  
Gabaergic Transmission ◽  
Chronic Alcohol Exposure

scholarly journals Linking brain connectivity and behavior after acute and chronic alcohol exposure

2017 ◽  
Vol 22 (8) ◽  
pp. 1079-1079 ◽  
Author(s):  
E Shokri-Kojori ◽  
D Tomasi ◽  
C E Wiers ◽  
G-J Wang ◽  
N D Volkow
Keyword(s):  
Brain Connectivity ◽  
Alcohol Exposure ◽  
Chronic Alcohol ◽  
Chronic Alcohol Exposure ◽  
And Behavior
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