Enhanced bio-hydrogen production by the combination of dark- and photo-fermentation in batch culture

2010 ◽  
Vol 101 (14) ◽  
pp. 5325-5329 ◽  
Author(s):  
Bing-Feng Liu ◽  
Nan-Qi Ren ◽  
Guo-Jun Xie ◽  
Jie Ding ◽  
Wan-Qian Guo ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Batch Culture

Enhanced hydrogen production by Rhodopseudomonas palustris CQK 01 with ultra-sonication pretreatment in batch culture

2011 ◽  
Vol 102 (18) ◽  
pp. 8696-8699 ◽  
Author(s):  
Xun Zhu ◽  
Xuewang Xie ◽  
Qiang Liao ◽  
Yongzhong Wang ◽  
Duujong Lee
Keyword(s):  
Hydrogen Production ◽  
Batch Culture ◽  
Rhodopseudomonas Palustris

Hydrogen-Production of Hydrogen-Producing Bacteria at Different Temperatures in Batch Culture

2012 ◽  
Vol 512-515 ◽  
pp. 1400-1403 ◽  
Author(s):  
Zhi Qin ◽  
Dan Li
Keyword(s):  
Hydrogen Production ◽  
Batch Culture ◽  
Ph Value ◽  
Optimal Parameter ◽  
Enrichment Culture ◽  
Gas Production ◽  
Logarithmic Phase ◽  
Production Of Hydrogen ◽  
Different Temperatures ◽  
C 30

Energy crisis is paid more attention to its significance around the world. Hydrogen is considered the most potential alternate energy source due to the character of non-pollution and zero emissions. This paper researched the variation of hydrogen-producing rate, pH value and the proportion under five temperatures of 25°C, 30°C, 35°C, 40°C, 45°C through batch culture and the reasons of these appearance. And anaerobic hydrogen-producing bacteria’s isolation and enrichment culture was accomplished by Hungater’s anaerobic technique. The time of logarithmic phase was 24h, 16h, 12h, 20h and 28h and the stationary phase was 36h, 28h, 24h, 32h and 36h at 25°C, 30°C, 35°C, 40°C, 45°C. When the pH declined to 4.2-4.4, the hydrogen-production rate and the proportion all reached optimal state. The maximum proportion of hydrogen-production and total gas-production was 70.41% at 35°C. The optimal parameter was: the pH between 4.2-4.4 under the optimum temperature of 35°C.

Microbial conversion of arabinose and xylose to hydrogen by a newly isolated Clostridium sp. No. 2.

1994 ◽  
Vol 40 (3) ◽  
pp. 228-233 ◽  
Author(s):  
Fumiaki Taguchi ◽  
Naoki Mizukami ◽  
Katsushige Hasegawa ◽  
Tatsuo Saito-Taki
Keyword(s):  
Hydrogen Production ◽  
Batch Culture ◽  
Conversion Rate ◽  
Evolution Rate ◽  
Microbial Conversion ◽  
Maximum Value

For 24 h at pH 6.0 and 36 °C, Clostridium sp. No. 2, isolated from termites, converted arabinose and xylose to hydrogen with yields of 14.55 and 13.73 mmol/g of substrate consumed, respectively. These yields were greater than the maximum value of 11.07 mmol/g of glucose consumed in 1 L of batch culture with 10 g of each substrate. The organism produced hydrogen from 45 g of glucose and xylose in 1 L of culture with a total accumulation of 380.6 mmol from glucose and 479.0 mmol from xylose, at a conversion rate of 270.9 mmol/g of dry cells from glucose and 405.9 mmol/g of dry cells from xylose. Hydrogen was produced at a maximum evolution rate of 27.2 mmol/h after 8 h of incubation with glucose and of 28.6 mmol/h after 6 h of incubation with xylose.Key words: hydrogen production, xylose, arabinose, Clostridium sp. No. 2.

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