Probing electron transfer with Escherichia coli: A method to examine exoelectronics in microbial fuel cell type systems

2013 ◽  
Vol 148 ◽  
pp. 567-573 ◽  
Author(s):  
Marc Sugnaux ◽  
Sophie Mermoud ◽  
Ana Ferreira da Costa ◽  
Manuel Happe ◽  
Fabian Fischer
Keyword(s):  
Escherichia Coli ◽  
Electron Transfer ◽  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Type Systems ◽  
Cell Type

Outward electron transfer bySaccharomyces cerevisiaemonitored with a bi-cathodic microbial fuel cell-type activity sensor

Yeast ◽  
2009 ◽  
pp. n/a-n/a ◽  
Author(s):  
Raphaël Ducommun ◽  
Marie-France Favre ◽  
Delphine Carrard ◽  
Fabian Fischer
Keyword(s):  
Electron Transfer ◽  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Cell Type ◽  
Type Activity

scholarly journals Lack of Electricity Production by Pelobacter carbinolicus Indicates that the Capacity for Fe(III) Oxide Reduction Does Not Necessarily Confer Electron Transfer Ability to Fuel Cell Anodes

2007 ◽  
Vol 73 (16) ◽  
pp. 5347-5353 ◽  
Author(s):  
Hanno Richter ◽  
Martin Lanthier ◽  
Kelly P. Nevin ◽  
Derek R. Lovley
Keyword(s):  
Electron Transfer ◽  
Fuel Cells ◽  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Microbial Fuel Cells ◽  
Electron Donors ◽  
Rrna Genes ◽  
Anode Surface ◽  
Oxide Reduction ◽  
Fuel Cell Anodes

ABSTRACT The ability of Pelobacter carbinolicus to oxidize electron donors with electron transfer to the anodes of microbial fuel cells was evaluated because microorganisms closely related to Pelobacter species are generally abundant on the anodes of microbial fuel cells harvesting electricity from aquatic sediments. P. carbinolicus could not produce current in a microbial fuel cell with electron donors which support Fe(III) oxide reduction by this organism. Current was produced using a coculture of P. carbinolicus and Geobacter sulfurreducens with ethanol as the fuel. Ethanol consumption was associated with the transitory accumulation of acetate and hydrogen. G. sulfurreducens alone could not metabolize ethanol, suggesting that P. carbinolicus grew in the fuel cell by converting ethanol to hydrogen and acetate, which G. sulfurreducens oxidized with electron transfer to the anode. Up to 83% of the electrons available in ethanol were recovered as electricity and in the metabolic intermediate acetate. Hydrogen consumption by G. sulfurreducens was important for ethanol metabolism by P. carbinolicus. Confocal microscopy and analysis of 16S rRNA genes revealed that half of the cells growing on the anode surface were P. carbinolicus, but there was a nearly equal number of planktonic cells of P. carbinolicus. In contrast, G. sulfurreducens was primarily attached to the anode. P. carbinolicus represents the first Fe(III) oxide-reducing microorganism found to be unable to produce current in a microbial fuel cell, providing the first suggestion that the mechanisms for extracellular electron transfer to Fe(III) oxides and fuel cell anodes may be different.

Membrane‐electrode assembly enhances performance of a microbial fuel cell type biological oxygen demand sensor

2009 ◽  
Vol 30 (4) ◽  
pp. 329-336 ◽  
Author(s):  
Mia Kim ◽  
Moon Sik Hyun ◽  
Geoffrey M. Gadd ◽  
Gwang Tae Kim ◽  
Sang‐Joon Lee ◽  
...  
Keyword(s):  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Oxygen Demand ◽  
Membrane Electrode Assembly ◽  
Biological Oxygen Demand ◽  
Membrane Electrode ◽  
Cell Type

scholarly journals Generating Electricity Using Microbial Fuel Cell Powered by Benthic Mud Collected From Two Locations in Akure, Nigeria

2017 ◽  
Vol 13 (18) ◽  
pp. 242
Author(s):  
Adegunloye D. V ◽  
Olotu T. M
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Lactobacillus Plantarum ◽  
Salt Bridge ◽  
Peak Voltage ◽  
River Bed ◽  
Bacteria And Fungi ◽  
Set Up

Generating electricity using microbial fuel cell powered by benthic mud collected from two locations in Akure was carried out. The locations were Riverbed of FUTA and Apatapiti area of Akure. This was achieved by building anode and cathode containers connected together by a salt bridge and an external circuit was made to transfer the electrons from the anode to the cathode. Bacteria and fungi were isolated from the benthic mud for eight days using standard microbiological techniques. Lactobacillus plantarum, Escherichia coli, Bacillus subtilis, Enterobacter aerogenes, Trichoderma sp, Mucor sp and Alterania sp; Lactobacillus plantarum, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Myrothecium sp and Geotrichum candidum were bacteria and fungi isolated from the benthic mud of Apatapiti area and Riverbed of Futa, Akure respectively. This was used for the generation of electricity using unsterilized mud sample and the control setup was sterilized mud from same source. The set-up was monitored every 24hrous to determine the voltage and current generated. The pH, concentration and temperature were measured. The temperature remains constant throughout the experiment. The set-up were operated at a normal temperature of 27oC and 29oC for Riverbed of FUTA and Apatapiti area of Akure respectively. The peak voltage was between 182.5V and 192.5V and current produced from the main set-up was between 0.3A to 0.53A for Futa river bed while for Apatapiti area of Akure the peak voltage and current were 192.5V and 0.3A respectively. Higher microbial population, current and voltage were observed to be generated in River bed of Futa than Apatapiti area. The difference in the voltage and current and the control set-up shows that anaerobic microorganisms are capable of producing electricity from microbial fuel cell under appropriate conditions.

Heterocyclic aminopyrazine–reduced graphene oxide coated carbon cloth electrode as an active bio-electrocatalyst for extracellular electron transfer in microbial fuel cells

RSC Advances ◽  
2016 ◽  
Vol 6 (73) ◽  
pp. 68827-68834 ◽  
Author(s):  
Praveena Gangadharan ◽  
Indumathi M. Nambi ◽  
Jaganathan Senthilnathan ◽  
Pavithra V. M.
Keyword(s):  
Electron Transfer ◽  
Graphene Oxide ◽  
Fuel Cell ◽  
Reduced Graphene Oxide ◽  
Microbial Fuel Cell ◽  
Microbial Fuel Cells ◽  
Extracellular Electron Transfer ◽  
Carbon Cloth ◽  
Reduced Graphene ◽  
Coated Carbon

In the present study, a low molecular heterocyclic aminopyrazine (Apy)–reduced graphene oxide (r-GO) hybrid coated carbon cloth (r-GO–Apy–CC) was employed as an active and stable bio-electro catalyst in a microbial fuel cell (MFC).

A novel mediatorless microbial fuel cell based on direct biocatalysis of Escherichia coli

2006 ◽  
pp. 2257 ◽  
Author(s):  
Tian Zhang ◽  
Changzheng Cui ◽  
Shengli Chen ◽  
Xinping Ai ◽  
Hanxi Yang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fuel Cell ◽  
Microbial Fuel Cell

Factors affecting the performance of a single-chamber microbial fuel cell-type biological oxygen demand sensor

2013 ◽  
Vol 68 (9) ◽  
pp. 1914-1919 ◽  
Author(s):  
Gai-Xiu Yang ◽  
Yong-Ming Sun ◽  
Xiao-Ying Kong ◽  
Feng Zhen ◽  
Ying Li ◽  
...  
Keyword(s):  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Microbial Fuel Cells ◽  
Low Cost ◽  
Oxygen Demand ◽  
Response Signal ◽  
Cell Type ◽  
Single Chamber ◽  
Factors Affecting ◽  
Stable Voltage

Microbial fuel cells (MFCs) are devices that exploit microorganisms as biocatalysts to degrade organic matter or sludge present in wastewater (WW), and thereby generate electricity. We developed a simple, low-cost single-chamber microbial fuel cell (SCMFC)-type biochemical oxygen demand (BOD) sensor using carbon felt (anode) and activated sludge, and demonstrated its feasibility in the construction of a real-time BOD measurement system. Further, the effects of anodic pH and organic concentration on SCMFC performance were examined, and the correlation between BOD concentration and its response time was analyzed. Our results demonstrated that the SCMFC exhibited a stable voltage after 132 min following the addition of synthetic WW (BOD concentration: 200 mg/L). Notably, the response signal increased with an increase in BOD concentration (range: 5–200 mg/L) and was found to be directly proportional to the substrate concentration. However, at higher BOD concentrations (>120 mg/L) the response signal remained unaltered. Furthermore, we optimized the SCMFC using synthetic WW, and tested it with real WW. Upon feeding real WW, the BOD values exhibited a standard deviation from 2.08 to 8.3% when compared to the standard BOD5 method, thus demonstrating the practical applicability of the developed system to real treatment effluents.

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