High-titer lactic acid production from NaOH-pretreated corn stover by Bacillus coagulans LA204 using fed-batch simultaneous saccharification and fermentation under non-sterile condition

2015 ◽  
Vol 182 ◽  
pp. 251-257 ◽  
Author(s):  
Jinlong Hu ◽  
Zhenting Zhang ◽  
Yanxu Lin ◽  
Shumiao Zhao ◽  
Yuxia Mei ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Corn Stover ◽  
Acid Production ◽  
Simultaneous Saccharification And Fermentation ◽  
High Titer ◽  
Lactic Acid Production ◽  
Bacillus Coagulans ◽  
Fed Batch ◽  
Sterile Condition ◽  
Simultaneous Saccharification

scholarly journals High-Titer Lactic Acid Production by Pediococcus acidilactici PA204 from Corn Stover through Fed-Batch Simultaneous Saccharification and Fermentation

2020 ◽  
Vol 8 (10) ◽  
pp. 1491
Author(s):  
Zhenting Zhang ◽  
Yanan Li ◽  
Jianguo Zhang ◽  
Nan Peng ◽  
Yunxiang Liang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Corn Stover ◽  
High Efficiency ◽  
Simultaneous Saccharification And Fermentation ◽  
High Titer ◽  
Lactic Acid Production ◽  
Pediococcus Acidilactici ◽  
Fed Batch ◽  
Ssf Process ◽  
Simultaneous Saccharification

Lignocellulose comprised of cellulose and hemicellulose is one of the most abundant renewable feedstocks. Lactic acid bacteria have the ability to ferment sugar derived from lignocellulose. In this study, Pediococcus acidilactici PA204 is a lactic acid bacterium with a high tolerance of temperature and high-efficiency utilization of xylose. We developed a fed-batch simultaneous saccharification and fermentation (SSF) process at 37 °C (pH 6.0) using the 30 FPU (filter paper units)/g cellulase and 20 g/L corn steep powder in a 5 L bioreactor to produce lactic acid (LA). The titer, yield, and productivity of LA produced from 12% (w/w) NaOH-pretreated and washed stover were 92.01 g/L, 0.77 g/g stover, and 1.28 g/L/h, respectively, and those from 15% NaOH-pretreated and washed stover were 104.11 g/L, 0.69 g/g stover, and 1.24 g/L/h, respectively. This study develops a feasible fed-batch SSF process for LA production from corn stover and provides a promising candidate strain for high-titer and -yield lignocellulose-derived LA production.

scholarly journals Biodetoxification of Phenolic Inhibitors from Lignocellulose Pretreatment using Kurthia huakuii LAM0618T and Subsequent Lactic Acid Fermentation

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2626 ◽  
Author(s):  
Yuejiao Xie ◽  
Qing Hu ◽  
Guodong Feng ◽  
Xu Jiang ◽  
Jinlong Hu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
High Titer ◽  
Bacillus Coagulans ◽  
High Yield ◽  
Acid Fermentation ◽  
Biochemical Production ◽  
Water Rinsing ◽  
Simultaneous Saccharification

Phenolic inhibitors generated during alkaline pretreatment of lignocellulosic biomasses significantly hinder bacterial growth and subsequent biofuel and biochemical production. Water rinsing is an efficient method for removing these compounds. Nevertheless, this method often generates a great amount of wastewater, and leads to the loss of solid fiber particles and fermentable sugars. Kurthia huakuii LAM0618T, a recently identified microorganism, was herein shown to be able to efficiently transform phenolic compounds (syringaldehyde, hydroxybenzaldehyde, and vanillin) into less toxic acids. Taking advantage of these properties, a biodetoxification method was established by inoculating K. huakuii LAM0618T into the NH3/H2O2-pretreated unwashed corn stover to degrade phenolic inhibitors and weak acids generated during the pretreatment. Subsequently, 33.47 and 17.91 g/L lactic acid was produced by Bacillus coagulans LA204 at 50 °C through simultaneous saccharification and fermentation (SSF) from 8% (w/w) of NH3/H2O2-pretreated corn stover with or without K. huakuii LAM0618T-biodetoxification, indicating biodetoxification significantly increased lactic acid titer and yield. Importantly, using 15% (w/w) of the NH3/H2O2-pretreated K. huakuii LAM0618T-biodetoxified corn stover as a substrate through fed-batch simultaneous saccharification and fermentation, high titer and high yield of lactic acid (84.49 g/L and 0.56 g/g corn stover, respectively, with a productivity of 0.88 g/L/h) were produced by Bacillus coagulans LA204. Therefore, this study reported the first study on biodetoxification of alkaline-pretreated lignocellulosic material, and this biodetoxification method could replace water rinsing for removal of phenolic inhibitors and applied in biofuel and biochemical production using the alkaline-pretreated lignocellulosic bioresources.

Efficient l-lactic acid production from sweet sorghum bagasse by open simultaneous saccharification and fermentation

RSC Advances ◽  
2016 ◽  
Vol 6 (42) ◽  
pp. 35771-35777 ◽  
Author(s):  
Y. Wang ◽  
M. Wang ◽  
D. Cai ◽  
B. Wang ◽  
Z. Wang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Sweet Sorghum ◽  
Simultaneous Saccharification And Fermentation ◽  
Lactic Acid Production ◽  
Sweet Sorghum Bagasse ◽  
Sorghum Bagasse ◽  
Ssf Process ◽  
Simultaneous Saccharification

An open SSF process using B. coagulans LA1507 introduces an effective way to produce l-lactic acid from abundant SSB.

scholarly journals Lactic acid production from lime-treated wheat straw by Bacillus coagulans: neutralization of acid by fed-batch addition of alkaline substrate

2008 ◽  
Vol 78 (5) ◽  
pp. 751-758 ◽  
Author(s):  
Ronald H. W. Maas ◽  
Robert R. Bakker ◽  
Mickel L. A. Jansen ◽  
Diana Visser ◽  
Ed de Jong ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Wheat Straw ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Bacillus Coagulans ◽  
Fed Batch
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