Oxygen control and stressor treatments for complete and long-term suppression of nitrite-oxidizing bacteria in biofilm-based partial nitritation/anammox

2021 ◽  
pp. 125996
Author(s):  
Michiel Van Tendeloo ◽  
Yankai Xie ◽  
Wannes Van Beeck ◽  
Weiqiang Zhu ◽  
Sarah Lebeer ◽  
...  
Keyword(s):  
Oxygen Control ◽  
Partial Nitritation ◽  
Nitrite Oxidizing Bacteria

Long-term continuous partial nitritation-anammox reactor aeration optimization at different nitrogen loading rates for the treatment of ammonium rich digestate lagoon supernatant

2020 ◽  
Vol 99 ◽  
pp. 139-146
Author(s):  
Sen Yang ◽  
Shengnan Xu ◽  
Riccardo Boiocchi ◽  
Abdul Mohammed ◽  
Xinru Li ◽  
...  
Keyword(s):  
Nitrogen Loading ◽  
Partial Nitritation ◽  
Loading Rates

scholarly journals Partial nitritation treating nitrogen in old landfill leachate

2016 ◽  
Vol 19 (4) ◽  
pp. 39-49
Author(s):  
Nhat The Phan ◽  
Van Thi Thanh Truong ◽  
Son Thanh Le ◽  
Biec Nhu Ha ◽  
Dan Phuoc Nguyen
Keyword(s):  
Landfill Leachate ◽  
Retention Time ◽  
Sequencing Batch Reactor ◽  
Hydraulic Retention Time ◽  
Batch Reactor ◽  
Partial Nitritation ◽  
Time Operation ◽  
Nitrite Oxidizing Bacteria ◽  
Long Time ◽  
H 30

In this study, a lab-scale Partial Nitritation Sequencing Batch Reactor (PNSBR) was implemented for treating high-ammonium old landfill leachate to yield an appropriate NO2—N/ NH4+-N ratio from 1/1 to 1.32/1 mixture as a pretreatment for subsequent Anammox. The objective of this study was to determine the optimal hydraulic retention time (HRT) at different influent ammonia concentrations for 210 days. The experimental results showed that with the influent ammonia concentrations of 500, 1000, 1500 and 2000 mg/L, HRT is 12 h, 21 h, 30 h and 48 h, respectively. The range of free ammonia (FA) concentration from 17 to 44 mg/L completely inhibited nitrite oxidizing bacteria (NOB) for long time operation. The COD removal efficiency was very low (6±2) %.

scholarly journals Úsporné odstraňování dusíku procesem anammox z kalových a splaškových odpadních vod

Entecho ◽  
2019 ◽  
Vol 2 (1) ◽  
pp. 1-5
Author(s):  
Vojtěch Kouba ◽  
Jan Bartáček
Keyword(s):  
Nitrogen Removal ◽  
Low Temperatures ◽  
Municipal Wastewater ◽  
Pilot Scale ◽  
Main Stream ◽  
Excess Sludge ◽  
Partial Nitritation ◽  
Loading Rates ◽  
Reject Water ◽  
Nitrite Oxidizing Bacteria

Proces částečná nitritace-anammox odstraňuje amoniakální dusík z odpadních vod s polovičními náklady na aeraci, až o 80 % nižší produkcí přebytečného kalu a bez spotřeby organického substrátu. Jde o zavedený proces pro odstraňování dusíku z kalových vod z anaerobní fermentace, a podobně koncentrovaných a teplých odpadních vod. Na tyto vody se částečná nitritace-anammox aplikuje již déle než deset let, a to např. pod názvy ANAMMOX®, ANITA™ Mox, DEMON®, nebo TERRAMOX®. Optimalizované provozy těchto technologií dusík běžně odstraňují při zatížení 0,5–2,3 kg∙m–3∙d–1 (30–35 °C). Současnou výzvou pro výzkum je implementace částečné nitritace-anammox do hlavního proudu studené splaškové odpadní vody, přičemž konkrétními problémy jsou (i) potlačení nežádoucích nitratačních mikroorganismů (NOB) a (ii) adaptace mikroorganismů anammox na nízké teploty. Náš výzkum jsme začali s jednostupňovým procesem, a poté nitritaci a anammox rozdělili do dvou reaktorů. Prezentujeme strategii, která v laboratorním měřítku NOB účinně potlačila i při 12 °C a dále i v pilotním měřítku při 13–30 °C. Dále ukazujeme, že anammox je možné na nízké teploty adaptovat studenými šoky. Tyto výsledky umožní rozšířit úsporné odstraňování dusíku i do hlavního proudu splaškové odpadní vody na ČOV. English: Partial nitritation-anammox (PN/A) process removes nitrogen from wastewater with 50% reduction of aeration costs, 80% less excess sludge and no consumption of organic carbon. PN/A is an established process for the removal of nitrogen from reject water from anaerobic digestion and other similarly warm and concentrated streams. On such wastewater, PN/A has been applied in full scale for over 10 years under names such as ANAMMOX®, ANITA™ Mox, DEMON® or TERRAMOX®, whose optimized installations consistently achieve nitrogen removal loading rates of 0,5–2,3 kg∙m–3∙d–1. The current challenge for research is to implement PN/A into the main stream of cold municipal wastewater, the specific challenges being (i) suppression of undesirable nitrite oxidizing bacteria (NOB) and (ii) adaptation of anammox microorganisms to low temperatures. Our initial experiences with one-stage PN/A in the main stream led us to the separation of PN/A in two subsequent reactors. Subsequently, we developed a strategy for NOB suppression in partial nitritation even under 12 °C, which we then successfully tested in the pilot scale. Furthermore, we found that anammox can be adapted to low temperatures using cold shocks. In sum, these results will enable extending the savings for nitrogen removal into the main stream of wastewater at WWTP.

Storage without nitrite or nitrate enables the long-term preservation of full-scale partial nitritation/anammox sludge

2021 ◽  
pp. 151330
Author(s):  
Weiqiang Zhu ◽  
Michiel Van Tendeloo ◽  
Yankai Xie ◽  
Marijn Juliaan Timmer ◽  
Lai Peng ◽  
...  
Keyword(s):  
Full Scale ◽  
Partial Nitritation ◽  
Long Term Preservation

Long term partial nitritation of anaerobically treated black water and the emission of nitrous oxide

2010 ◽  
Vol 44 (7) ◽  
pp. 2171-2178 ◽  
Author(s):  
M.S. de Graaff ◽  
G. Zeeman ◽  
H. Temmink ◽  
M.C.M. van Loosdrecht ◽  
C.J.N. Buisman
Keyword(s):  
Nitrous Oxide ◽  
Partial Nitritation ◽  
Black Water

scholarly journals Short and long term continuous hydroxylamine feeding in a granular sludge partial nitritation reactor.

2021 ◽  
pp. 117945
Author(s):  
Aina Soler-Jofra ◽  
Lisbeth Schmidtchen ◽  
Lluc Olmo ◽  
Mark C.M. van Loosdrecht ◽  
Julio Pérez
Keyword(s):  
Granular Sludge ◽  
Partial Nitritation ◽  
Short And Long Term

scholarly journals Functional degeneracy in translationally active Nitrospira populations confers resilience to out-selection in partial nitritation activated sludge

2021 ◽  
Author(s):  
Maxwell B.W. Madill ◽  
Yaqian Luo ◽  
Pranav Sampara ◽  
Ryan M. Ziels
Keyword(s):  
Activated Sludge ◽  
Ecological Niches ◽  
Nitrite Accumulation ◽  
Partial Nitritation ◽  
Physiological Diversity ◽  
Translational Activity ◽  
Subsequent Decrease ◽  
Nitrite Oxidizing Bacteria ◽  
Canonical Amino Acid

AbstractEnergy-efficient nitrogen removal in activated sludge wastewater treatment systems could be achieved by oxidizing a part of the influent ammonium to nitrite (partial nitritation; PN) to provide growth substrates for anammox. However, the metabolic and physiological diversity of nitrite oxidizing bacteria (NOB) makes their out-selection a challenge in mainstream activated sludge, warranting measurements of their in situ physiology and activity under selective growth pressures. Here, we examined the long-term (~100 day) stability of PN in mainstream activated sludge obtained by treating a portion of return activated sludge with a sidestream flow containing free ammonia (FA) at 200 mg NH3-N/L. The nitrite accumulation ratio peaked at 42% by day 40 in the reactor with sidestream FA-treatment, while it did not increase in the control reactor without FA added to the sidestream. A subsequent decrease in nitrite accumulation within the FA-treated reactor coincided with shifts in dominant Nitrospira 16S rRNA amplicon sequence variants (ASVs). We applied bioorthogonal non-canonical amino acid tagging (BONCAT) coupled with fluorescence activated cell sorting (FACS) to investigate changes in the translational activity of NOB populations throughout sidestream exposure to FA. BONCAT-FACS confirmed that the single Nitrospira ASV out-selected in the FA-treated reactor had reduced translational activity following exposure to FA, whereas the two Nitrospira ASVs that emerged after process acclimation were not impacted by FA. Thus, functionally degenerate Nitrospira populations likely provided resilience to the nitrite-oxidizing community during FA-treatment by shifting activity to physiologically resistant members. These results highlight how in situ physiology approaches like BONCAT can resolve ecological niches in the activated sludge microbiome, and how functional degeneracy observed within Nitrospira populations likely necessitates a combination of out-selection strategies to achieve stable mainstream PN.

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