Choline oxidase as a selective recognition element for determination of paraoxon

2009 ◽  
Vol 24 (8) ◽  
pp. 2509-2514 ◽  
Author(s):  
Sharareh Sajjadi ◽  
Hedayatollah Ghourchian ◽  
Hassan Tavakoli
Keyword(s):  
Choline Oxidase ◽  
Selective Recognition ◽  
Recognition Element

scholarly journals Determination of Choline in Milk and Infant Formulas by Enzymatic Analysis: Collaborative Study

2000 ◽  
Vol 83 (1) ◽  
pp. 131-138 ◽  
Author(s):  
David C Woollard ◽  
Harvey E Indyk
Keyword(s):  
Acid Hydrolysis ◽  
Infant Formula ◽  
Spectrophotometric Method ◽  
Collaborative Study ◽  
Analytical Techniques ◽  
Choline Oxidase ◽  
Infant Formulas ◽  
Enzymatic Analysis ◽  
Data Screening

Abstract A collaborative study was conducted on a coupled enzymatic–spectrophotometric method for the determination of choline in infant formula and milk powders. Twenty-nine laboratories participated in the analysis of 8 blind duplicates over the range of 45–175 mg/100 g sample. After the combined acid hydrolysis–phospholipase release of choline, incubation with choline oxidase in the presence of peroxidase and phenol generates a quinoneimine chromophore with 4-aminoantipyrine. Following raw data screening, overall mean RSDR was estimated at 5.14% (range, 4.26–6.34%) with a HORRAT value of 0.91 (range, 0.76–1.00) and an overall mean RSDr:RSDR value of 0.53 (range, 0.42–0.74). The method was also compared with alternative independent analytical techniques for several of the collaborative study samples.

Luminescent behavior of pyrene-allied calix[4]arene for the highly pH-selective recognition and determination of Zn2+, Hg2+ and I−via the CHEF-PET mechanism: computational experiment and paper-based device

2019 ◽  
Vol 43 (25) ◽  
pp. 9855-9864 ◽  
Author(s):  
Pinkesh G. Sutariya ◽  
Heni Soni ◽  
Sahaj A. Gandhi ◽  
Alok Pandya
Keyword(s):  
Binding Sites ◽  
Computational Experiment ◽  
Fluorescence Sensor ◽  
Selective Recognition ◽  
First Time ◽  
Luminescent Behavior

In this article, for the first time, we have reported a novel CHEF-PET fluorescence sensor L based on calix[4]arene containing four pyrene groups as binding sites, which is highly selective and sensitive towards Zn2+, Hg2+ and I−.

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