A dumbell probe-mediated rolling circle amplification strategy for highly sensitive transcription factor detection

2015 ◽  
Vol 64 ◽  
pp. 505-510 ◽  
Author(s):  
Chunxiang Li ◽  
Xiyang Qiu ◽  
Zhaohui Hou ◽  
Keqin Deng
Keyword(s):  
Transcription Factor ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Highly Sensitive ◽  
Amplification Strategy

Target-responsive dumbbell probe-mediated rolling circle amplification strategy for highly sensitive Hg2+ detection

RSC Advances ◽  
2014 ◽  
Vol 4 (51) ◽  
pp. 27091-27097 ◽  
Author(s):  
Qingwang Xue ◽  
Yanqin Lv ◽  
Yuanfu Zhang ◽  
Shuling Xu ◽  
Qiaoli Yue ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Selective Detection ◽  
Label Free ◽  
Rolling Circle ◽  
Fluorescent Sensing ◽  
Highly Sensitive ◽  
Mercuric Ions ◽  
Amplification Strategy

A novel label-free amplified fluorescent sensing scheme based on target-responsive dumbbell probe-mediated rolling circle amplification (D-RCA) has been developed for sensitive and selective detection of mercuric ions.

G-quadruplex fluorescent probe-mediated real-time rolling circle amplification strategy for highly sensitive microRNA detection

2016 ◽  
Vol 943 ◽  
pp. 114-122 ◽  
Author(s):  
Hong-Xin Jiang ◽  
Zhen-Zhen Liang ◽  
Yan-Hong Ma ◽  
De-Ming Kong ◽  
Zhang-Yong Hong
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
G Quadruplex ◽  
Amplification Strategy

scholarly journals A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection

2010 ◽  
Vol 38 (15) ◽  
pp. e156-e156 ◽  
Author(s):  
Yuntao Zhou ◽  
Qing Huang ◽  
Jimin Gao ◽  
Jianxin Lu ◽  
Xizhong Shen ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Amplification Strategy

A DNA logic gate based on strand displacement reaction and rolling circle amplification, responding to multiple low-abundance DNA fragment input signals, and its application in detecting miRNAs

2015 ◽  
Vol 51 (32) ◽  
pp. 6980-6983 ◽  
Author(s):  
Yuqi Chen ◽  
Yanyan Song ◽  
Fan Wu ◽  
Wenting Liu ◽  
Boshi Fu ◽  
...  
Keyword(s):  
Logic Gate ◽  
Rolling Circle Amplification ◽  
Displacement Reaction ◽  
Strand Displacement ◽  
Rolling Circle ◽  
Highly Sensitive ◽  
Dna Fragment ◽  
Highly Sensitive Detection ◽  
Amplification Strategy ◽  
Strand Displacement Reaction

A conveniently amplified DNA AND logic gate platform was designed for the highly sensitive detection of low-abundance DNA fragment inputs based on strand displacement reaction and rolling circle amplification strategy.

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

scholarly journals Highly sensitive detection of Staphylococcus aureus by a THz metamaterial biosensor based on gold nanoparticles and rolling circle amplification

RSC Advances ◽  
2020 ◽  
Vol 10 (45) ◽  
pp. 26824-26833 ◽  
Author(s):  
Ke Yang ◽  
Wenjing Yu ◽  
Guorong Huang ◽  
Jie Zhou ◽  
Xiang Yang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Hospital Acquired Infections ◽  
Food Borne ◽  
Highly Sensitive ◽  
Food Borne Illness ◽  
Highly Sensitive Detection ◽  
Hospital Acquired ◽  
The Impact

A highly sensitive method for detecting Staphylococcus aureus (S. aureus) is urgently needed to reduce the impact and spread of hospital-acquired infections and food-borne illness.

Molecular Beacon Enables Combination of Highly Processive and Highly Sensitive Rolling Circle Amplification Readouts for Detection of DNA-Modifying Enzymes

Nano LIFE ◽  
2015 ◽  
Vol 05 (02) ◽  
pp. 1541002 ◽  
Author(s):  
Emil L. Kristoffersen ◽  
Maria Gonzalez ◽  
Magnus Stougaard ◽  
Cinzia Tesauro
Keyword(s):  
Real Time ◽  
Dna Cleavage ◽  
Topoisomerase I ◽  
Drug Response ◽  
Molecular Beacon ◽  
Rolling Circle Amplification ◽  
Dna Topoisomerase ◽  
Rolling Circle ◽  
Topoisomerase Ib ◽  
Highly Sensitive

Here we present an optimized readout format for detection of the circularized products from a DNA-based sensor for measurement of DNA-modifying enzymes including DNA Topoisomerase I. The basic design of the DNA-sensor relies on the use of a substrate that can be circularized by the activity of DNA-modifying enzymes like type IB Topoisomerases and subsequently amplified by a rolling circle amplification (RCA) mechanism. The RCA process can be followed in real-time by the addition of a molecular beacon with a fluorophore/quencher pair. Upon hybridization to the amplified product, the fluorophore/quencher pair is separated, giving rise to a fluorescent signal, measurable in pseudo real-time using a qPCR machine or in a fluorimeter. The RCA products in complex with the molecular beacon can subsequently be moved to microscopic slides and analyzed in a fluorescence microscope. We describe the proof of the principle of this molecular beacon-based method combining the qPCR readout format with the standard Rolling circle Enhanced Enzymatic Assay previously reported. Although the qPCR setup is less sensitive, it allows easy, fast, and high-throughput measurement of enzyme activities. Human Topoisomerase IB (TopIB) is a well-known target for the clinically used anticancer drugs of the camptothecin family. The cytotoxic effect of camptothecins correlates directly with the intracellular TopIB activity affecting reversibly the Topoisomerase/DNA cleavage complexes. Therefore, we envisioned that the presented method may find use for the prediction of cellular drug response and for drug screening to discover novel molecules that specifically inhibit TopIB or other DNA-modifying enzymes.

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