Indirect electrochemical detection for total bile acids in human serum

Introduction: Intrahepatic cholestasis of pregnancy (ICP) is the liver disorder in the second or early third trimester of pregnancy. It is characterized by pruritus with increased serum bile acids concentration and other liver function tests. ICP is connected with increased risk of fetal mortality, but is unfortunately detected quite late. Therefore, it is important to recognize the disease in its early stages. We aimed to investigate the serum alcohol dehydrogenase (ADH) activity and compare it with the concentration of total bile acid (TBA) in women with ICP. Methods: Serum samples were taken for routine investigation from 80 pregnancies with ICP in the second or third trimester of pregnancy and from 80 healthy pregnant women in the same time of pregnancy. For measurement of class I activity we used the spectrofluorometric methods. The total ADH activitiy was measured by the photometric method. Results: The analysis of results shows a statistically significant increase in the activity of ADH I and ADH total (about 60% and 41.3%, respectively). Activity of ADH I well correlated with aminotransferases (alanine ALT and aspartate AST) and total bile acids (TBA) concentration. The total ADH activity was also positively correlated with ALT, AST and total bile acids. Conclusion: We can state that the activity of class I alcohol dehydrogenase isoenzyme in the sera of patients with ICP is increased and seems to be a good indicator of liver cell destruction during this disease and is comparable with the value of other markers.

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Effects of Praziquantel Treatment on the Levels of Total Bile Acids and the Basement Membrane Formation in Schistosomiasis: Correlations with the Severity of the Disease

Bulletin of Egyptian Society for Physiological Sciences ◽

10.21608/besps.2009.36355 ◽

2009 ◽

Vol 29 (2) ◽

pp. 95-108

Author(s):

El-Shahat Tosona ◽

Kadry Gad

Keyword(s):

Basement Membrane ◽

Bile Acids ◽

Membrane Formation ◽

Praziquantel Treatment ◽

Severity Of The Disease ◽

Total Bile Acids

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Selective and Sensitive Electrochemical Detection of ATP in Human Serum Samples at ZrO2 Hallow Spheres Modified GCE

Journal of The Electrochemical Society ◽

10.1149/1945-7111/abef48 ◽

2021 ◽

Author(s):

K. Pandian ◽

kalaiyarasi J

Keyword(s):

Human Serum ◽

Electrochemical Detection ◽

Serum Samples ◽

Human Serum Samples

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Bile Acid and Fibroblast Growth Factor 19 Regulation in Obese Diabetics, and Non-Alcoholic Fatty Liver Disease after Sleeve Gastrectomy

Journal of Clinical Medicine ◽

10.3390/jcm8060815 ◽

2019 ◽

Vol 8 (6) ◽

pp. 815 ◽

Cited By ~ 18

Author(s):

Hsien-Hao Huang ◽

Wei-Jei Lee ◽

Shu-Chun Chen ◽

Tung-Fang Chen ◽

Shou-Dong Lee ◽

...

Keyword(s):

Bile Acid ◽

Fatty Liver ◽

Bile Acids ◽

Fatty Liver Disease ◽

Total Bile Acid ◽

Alcoholic Fatty Liver ◽

Liver Index ◽

Fatty Liver Index ◽

Total Bile Acids ◽

Alcoholic Fatty Liver Disease

Background: Sleeve gastrectomy (SG) is an effective treatment for obesity and type 2 diabetes mellitus (T2DM), and non-alcoholic fatty liver disease (NAFLD); however, the mechanism is not completely understood. Bile acids and fibroblast growth factors (FGFs) are involved in the regulation of energy metabolism. Methods: We investigated the roles of total bile acid and FGF 19 in T2DM remission and NAFLD improvement in obese subjects undergoing SG. A total of 18 patients with obesity and T2DM undergoing laparoscopic SG were enrolled in this study. Serial plasma total bile acid and FGF 19 levels were measured, while the fatty liver index was calculated before and after surgery. Results: The FGF 19 level significantly increased, and the total bile acid level and fatty liver index decreased 1 year after surgery. The complete T2DM remission rate was 66.7% one year after surgery; the complete remitters had significantly lower FGF 19 levels and higher insulin levels than the non-complete remitters. The complete remitters also had significantly decreased total bile acid levels and increased FGF 19 levels 1 year after surgery compared with those before surgery. The fatty improvers had significantly decreased total bile acid levels and increased FGF 19 levels 1 year after surgery compared with those before surgery. Conclusion: The total bile acids level and fatty liver index decreased, and the FGF 19 levels increased 1 year after SG. Both T2DM complete remitters and NAFLD improvers showed significantly decreased total bile acid levels and increased FGF 19 levels 1 year after SG. Plasma total bile acids and FGF 19 might have roles in T2DM remission and NAFLD improvement. Low preoperative FGF 19 levels might be a predictor for NAFLD improvement after SG.

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