Selective isolation of magnetic nanoparticle-mediated heterogeneity subpopulation of circulating tumor cells using magnetic gradient based microfluidic system

2017 ◽  
Vol 88 ◽  
pp. 153-158 ◽  
Author(s):  
Bongseop Kwak ◽  
Jaehun Lee ◽  
Dongkyu Lee ◽  
Kangho Lee ◽  
Ohwon Kwon ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Magnetic Nanoparticle ◽  
Microfluidic System ◽  
Selective Isolation ◽  
Magnetic Gradient ◽  
Gradient Based

scholarly journals Functional profiling of circulating tumor cells with an integrated vortex capture and single-cell protease activity assay

2018 ◽  
Vol 115 (40) ◽  
pp. 9986-9991 ◽  
Author(s):  
Manjima Dhar ◽  
Jeffrey Nam Lam ◽  
Tonya Walser ◽  
Steven M. Dubinett ◽  
Matthew B. Rettig ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Activity Ratio ◽  
Proteolytic Enzymes ◽  
Microfluidic System ◽  
Matrix Metalloprotease ◽  
Rare Cancer ◽  
Companion Diagnostics ◽  
Functional Profiling ◽  
Vortex Capture

Tumor cells are hypothesized to use proteolytic enzymes to facilitate invasion. Whether circulating tumor cells (CTCs) secrete these enzymes to aid metastasis is unknown. A quantitative and high-throughput approach to assay CTC secretion is needed to address this question. We developed an integrated microfluidic system that concentrates rare cancer cells >100,000-fold from 1 mL of whole blood into ∼50,000 2-nL drops composed of assay reagents within 15 min. The system isolates CTCs by size, exchanges fluid around CTCs to remove contaminants, introduces a matrix metalloprotease (MMP) substrate, and encapsulates CTCs into microdroplets. We found CTCs from prostate cancer patients possessed above baseline levels of MMP activity (1.7- to 200-fold). Activity of CTCs was generally higher than leukocytes from the same patient (average CTC/leukocyte MMP activity ratio, 2.6 ± 1.5). Higher MMP activity of CTCs suggests active proteolytic processes that may facilitate invasion or immune evasion and be relevant phenotypic biomarkers enabling companion diagnostics for anti-MMP therapies.

scholarly journals Integrative Analysis and Machine Learning based Characterization of Single Circulating Tumor Cells

2020 ◽  
Vol 9 (4) ◽  
pp. 1206 ◽  
Author(s):  
Arvind Iyer ◽  
Krishan Gupta ◽  
Shreya Sharma ◽  
Kishore Hari ◽  
Yi Fang Lee ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Expression Profiles ◽  
Gene Expression Pattern ◽  
Microfluidic System ◽  
Integrative Analysis ◽  
Label Free ◽  
Peripheral Blood Mononuclear ◽  
Cell Expression

We collated publicly available single-cell expression profiles of circulating tumor cells (CTCs) and showed that CTCs across cancers lie on a near-perfect continuum of epithelial to mesenchymal (EMT) transition. Integrative analysis of CTC transcriptomes also highlighted the inverse gene expression pattern between PD-L1 and MHC, which is implicated in cancer immunotherapy. We used the CTCs expression profiles in tandem with publicly available peripheral blood mononuclear cell (PBMC) transcriptomes to train a classifier that accurately recognizes CTCs of diverse phenotype. Further, we used this classifier to validate circulating breast tumor cells captured using a newly developed microfluidic system for label-free enrichment of CTCs.

ClearCell FX Microfluidic System for the Enrichment and Genetic Analysis of Circulating Tumor Cells.

2015 ◽  
Vol 33 (15_suppl) ◽  
pp. e22023-e22023
Author(s):  
Sohan Lal Punia ◽  
LIntao Bi ◽  
Mehmet Akif Turker ◽  
Jin Sung Jang ◽  
Michael B Campion ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic System

Continuous labeling of circulating tumor cells with microbeads using a vortex micromixer for highly selective isolation

2013 ◽  
Vol 40 (1) ◽  
pp. 63-67 ◽  
Author(s):  
Ming Xian Lin ◽  
Kyung-A Hyun ◽  
Hui-Sung Moon ◽  
Tae Seok Sim ◽  
Jeong-Gun Lee ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Selective Isolation
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