2′-Fluoro ribonucleic acid modified DNA dual-probe sensing strategy for enzyme-amplified electrochemical detection of double-strand DNA of PML/RARα related fusion gene

AbstractCancer has proven to be an extremely difficult challenge to treat. Several fundamental issues currently underlie cancer treatment including differentiating self from non-self, functional coupling of the recognition and therapeutic components of various therapies, and the propensity of cancerous cells to develop resistance to common treatment modalities via evolutionary pressure. Given these limitations, there is an increasing need to develop an all-encompassing therapeutic that can uniquely target malignant cells, decouple recognition from treatment, and overcome evolutionarily driven cancer resistance. We describe herein, a new class of programmable self-assembling dsRNA-based cancer therapeutics, that uniquely targets aberrant genetic sequences, and in a functionally decoupled manner, undergoes oncogenic RNA activated displacement (ORAD), initiating a therapeutic cascade that induces apoptosis and immune activation. As a proof-of-concept, we show that RNA strands targeting the EWS/Fli1 fusion gene in Ewing Sarcoma cells that are end-blocked with phosphorothioate bonds and additionally sealed with a 2’-U modified DNA protector can be used to induce specific and potent killing of cells containing the target oncogenic sequence, but not wildtype.

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Fabrication and evaluation of [Co(phen)2L]3+-modified DNA-MWCNT and SDS-MWCNT electrodes for electrochemical detection of 6-mercaptopurine

Journal of Electroanalytical Chemistry ◽

10.1016/j.jelechem.2011.11.026 ◽

2012 ◽

Vol 665 ◽

pp. 63-69 ◽

Cited By ~ 20

Author(s):

Peina Zhou ◽

Leiqing He ◽

Guilian Gan ◽

Shenyan Ni ◽

Hong Li ◽

...

Keyword(s):

Electrochemical Detection ◽

Modified Dna

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A novel electrochemical DNA detection method based on bio-barcode/gold label silver stain dual amplification

Materials Express ◽

10.1166/mex.2020.1633 ◽

2020 ◽

Vol 10 (2) ◽

pp. 206-213

Author(s):

Pengfei Jiang ◽

Miduo Tan ◽

Lan Zhao ◽

Yulin Wang ◽

Ting Pan ◽

...

Keyword(s):

Electrochemical Detection ◽

Detection Method ◽

Linear Sweep Voltammetry ◽

Silica Nanoparticle ◽

Silver Ions ◽

Silver Deposition ◽

Silver Stain ◽

Modified Dna ◽

Gold Label ◽

Dual Amplification

A novel electrochemical gene detection method based on bio-barcode/gold label silver stain (GLSS) dual amplification is presented. The silica nanoparticle/gold nanoparticles (SiO2/AuNPs) barcode probes were constructed by combining SiO2 nanoparticle-labeled DNA with AuNPs, which hybridizes with target DNAs and the electrode surface-modified DNA probes. The silver ions were reduced on the surface of AuNPs to form a silver shell by the catalysis of AuNPs, and then the electrochemical detection of deposited silver was performed by linear sweep voltammetry (LSV). In this paper, the effects of electrode blocking agent, blocking time and silver deposition time on electrochemical detection were also investigated. The proposed electrochemical DNA biosensor bring forth a low detection limit of 0.23 fM and a widely linear range of 1 fM to 10 pM. This method can clearly distinguish the complementary and mismatched DNA, with good repeatability and stability.

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