A novel electrochemical DNA detection method based on bio-barcode/gold label silver stain dual amplification

A novel electrochemical gene detection method based on bio-barcode/gold label silver stain (GLSS) dual amplification is presented. The silica nanoparticle/gold nanoparticles (SiO2/AuNPs) barcode probes were constructed by combining SiO2 nanoparticle-labeled DNA with AuNPs, which hybridizes with target DNAs and the electrode surface-modified DNA probes. The silver ions were reduced on the surface of AuNPs to form a silver shell by the catalysis of AuNPs, and then the electrochemical detection of deposited silver was performed by linear sweep voltammetry (LSV). In this paper, the effects of electrode blocking agent, blocking time and silver deposition time on electrochemical detection were also investigated. The proposed electrochemical DNA biosensor bring forth a low detection limit of 0.23 fM and a widely linear range of 1 fM to 10 pM. This method can clearly distinguish the complementary and mismatched DNA, with good repeatability and stability.

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A highly sensitive high performance liquid chromatography-electrochemical detection method for the determination of five phenolic compounds from Salvia miltiorrhiza

Chinese Journal of Chromatography ◽

10.3724/sp.j.1123.2017.03038 ◽

2017 ◽

Vol 35 (8) ◽

pp. 897 ◽

Cited By ~ 2

Author(s):

Zhen Long ◽

Paul Gamache ◽

Zhimou Guo ◽

Yuanyuan Pan ◽

Yanhai Zhang ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Phenolic Compounds ◽

Electrochemical Detection ◽

High Performance ◽

Detection Method ◽

Salvia Miltiorrhiza ◽

Highly Sensitive

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Bimetallic Core–Shell Nanoparticles of Gold and Silver via Bioinspired Polydopamine Layer as Surface-Enhanced Raman Spectroscopy (SERS) Platform

Nanomaterials ◽

10.3390/nano10040688 ◽

2020 ◽

Vol 10 (4) ◽

pp. 688 ◽

Cited By ~ 4

Author(s):

Asli Yilmaz ◽

Mehmet Yilmaz

Keyword(s):

Raman Spectroscopy ◽

Low Cost ◽

Silver Ions ◽

Surface Enhanced Raman Spectroscopy ◽

Core Shell ◽

Silver Deposition ◽

Shell Nanoparticles ◽

Surface Enhanced ◽

Surface Enhanced Raman ◽

Core Shell Nanoparticles

Despite numerous attempts to fabricate the core–shell nanoparticles, novel, simple, and low-cost approaches are still required to produce these efficient nanosystems. In this study, we propose the synthesis of bimetallic core–shell nanoparticles of gold (AuNP) and silver (AgNP) nanostructures via a bioinspired polydopamine (PDOP) layer and their employment as a surface-enhanced Raman spectroscopy (SERS) platform. Herein, the PDOP layer was used as an interface between nanostructures as well as stabilizing and reducing agents for the deposition of silver ions onto the AuNPs. UV-vis absorption spectra and electron microscope images confirmed the deposition of the silver ions and the formation of core–shell nanoparticles. SERS activity tests indicated that both the PDOP thickness and silver deposition time are the dominant parameters that determine the SERS performances of the proposed core–shell system. In comparison to bare AuNPs, more than three times higher SERS signal intensity was obtained with an enhancement factor of 3.5 × 105.

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Advanced negative detection method comparable to silver stain for SDS-PAGE separated proteins detection

Analytical Biochemistry ◽

10.1016/j.ab.2016.07.015 ◽

2016 ◽

Vol 510 ◽

pp. 21-25 ◽

Cited By ~ 1

Author(s):

Xu Wang ◽

Sun-Young Hwang ◽

Wei-Tao Cong ◽

Li-Tai Jin ◽

Jung-Kap Choi

Keyword(s):

Detection Method ◽

Silver Stain ◽

Sds Page ◽

Negative Detection

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Ultrasensitive electrochemical immunosensor of carcinoembryonic antigen based on gold-label silver-stain signal amplification

Chinese Chemical Letters ◽

10.1016/j.cclet.2018.11.030 ◽

2018 ◽

Vol 29 (12) ◽

pp. 1857-1860 ◽

Cited By ~ 14

Author(s):

Gaojian Yang ◽

Yuxuan Lai ◽

Ziqi Xiao ◽

Congli Tang ◽

Yan Deng

Keyword(s):

Carcinoembryonic Antigen ◽

Signal Amplification ◽

Electrochemical Immunosensor ◽

Silver Stain ◽

Gold Label

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Manufacturing Process of Polymeric Microneedle Sensors for Mass Production

Micromachines ◽

10.3390/mi12111364 ◽

2021 ◽

Vol 12 (11) ◽

pp. 1364

Author(s):

Jae Yun Baek ◽

Kyung Mook Kang ◽

Hyeong Jun Kim ◽

Ju Hyeon Kim ◽

Ju Hwan Lee ◽

...

Keyword(s):

Cyclic Voltammetry ◽

Electrochemical Detection ◽

Mass Production ◽

Interstitial Fluid ◽

Linear Sweep Voltammetry ◽

Sensor Surface ◽

Linear Sweep ◽

Thermal Compression ◽

Stencil Mask ◽

Sensor Fabrication

In this work, we present a fabrication process for microneedle sensors made of polylactic acid (PLA), which can be utilized for the electrochemical detection of various biomarkers in interstitial fluid. Microneedles were fabricated by the thermal compression molding of PLA into a laser machined polytetrafluoroethylene (PTFE) mold. Sensor fabrication was completed by forming working, counter, and reference electrodes on each sensor surface by Au sputtering through a stencil mask, followed by laser dicing to separate individual sensors from the substrate. The devised series of processes was designed to be suitable for mass production, where multiple microneedle sensors can be produced at once on a 4-inch wafer. The operational stability of the fabricated sensors was confirmed by linear sweep voltammetry and cyclic voltammetry at the range of working potentials of various biochemical molecules in interstitial fluid.

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