Two-Photon Time-Gated In Vivo Imaging of Dihydrolipoic-Acid-Decorated Gold Nanoclusters

Due to the unique advantages of two-photon technology and time-resolved imaging technology in the biomedical field, attention has been paid to them. Gold clusters possess excellent physicochemical properties and low biotoxicity, which make them greatly advantageous in biological imaging, especially for in vivo animal imaging. A gold nanocluster was coupled with dihydrolipoic acid to obtain a functionalized nanoprobe; the material displayed significant features, including a large two-photon absorption cross-section (up to 1.59 × 105 GM) and prolonged fluorescence lifetime (>300 ns). The two-photon and time-resolution techniques were used to perform cell imaging and in vivo imaging.

Application of encapsulated hollow Gold nanocluster targets for high-quality and quasi-monoenergetic ions generation

With persistent progress in ultra-intense laser pulses, Coulomb explosion (CE) of spherical nanoclusters can in principle produce high-quality-quasi-monoenergetic ions. Focusing on using CE framework, in this paper, we have proposed a target scheme to accelerate light/heavy ions’ beam. The scheme relies on encapsulating a hollow Gold nanocluster inside a hollow proton-Carbon (HC) nanosphere. The ability of this suggestion has been simulated by the two-dimensional particle-in-cell code (EPOCH). Simulation results exhibit that a hollow Gold cluster can positively increase the electrons’ extraction. This condition may improve the acceleration of low-divergence H+, C6+, and Au67+ ions. Our simulation shows that at the end of the interaction, for a Gold cluster with an optimal hollow radius of 91.3 nm, the cut-off energy of H+, C6+, and Au67+ are about 54.9 MeV/u, 51.5 MeV/u, and 54.9 MeV/u, respectively. In this case, an increase of about 52% for H+ and 61% for C6+ is obtained, contrast to bare HC hollow nanosphere (i.e., a hollow nanosphere with no cluster), while the relative divergence decreases to 1.38 and 1.86, respectively for H+ and C6+ ions. We have also compared our simulation results with another proposed target structure composed of a void area with an optimum diameter of 70.4 nm between the fully- Gold nanocluster and HC nanosphere. We have exhibited that the results are improved, contrast to bare nanosphere. However, the cut-off energy suppression and angular divergence increase are shown compared with encapsulated hollow Gold nanocluster structure.

Spider Toxin Peptide-Induced NIR Gold Nanocluster Fabrication for GSH-Responsive Cancer Cell Imaging and Nuclei Translocation

Goldnanoclusters (GNCs) have become a promising nanomaterial for bioimaging because of their unique optical properties and biocompatibility. In this study, lycosin-I peptide, which possesses a highly selective anticancer activity by affecting the permeability of cancer cell membrane, was firstly modified for constructing fluorescent GNCs (LGNCs) for bioimaging of tumor cells. The obtained LGNCs exhibited strong near-infrared (NIR) fluorescence, which can be further enhanced by the peptide-induced aggregation and selectively stained three cancerous cell lines over normal cell lines with low intrinsic toxicity. After uptake by tumor cells, LGNC aggregates can be depolymerized into ultrasmall nanoclusters by high-level glutathione (GSH) and realize the nuclear targeting translocation. Collectively, our work suggests the potential of natural active biomolecules in designing NIR fluorescent GNCs for bioimaging.

Adsorption Behavior of an Adamantane Derivative on Metal Clusters – DFT Simulation Studies

Investigation of the adsorption properties of 3-(adamantan-1-yl)-4-phenyl-1-[(4-phenylpiperazin-yl)methyl]-1H-1,2,4-triazole-5(4H)-thione (APT) with metal clusters (mC: Ag, Au and Cu) are reported using DFT method. APT is found to form stable cluster with transition metal clusters of copper, silver and gold. The drug-cluster complexation energy is slightly more for the gold nanocluster-drug complex. Dipole moment of the drug-gold cluster is found to be higher than that of the other systems. SERS studies demonstrate improved Raman signals for multiple wavenumbers of all APT-metal cluster complexes. Different spectroscopic, chemical and electronic properties are also investigated.