Development of microfluidic impedance cytometry enabling the quantification of specific membrane capacitance and cytoplasm conductivity from 100,000 single cells

2018 ◽  
Vol 111 ◽  
pp. 138-143 ◽  
Author(s):  
Yang Zhao ◽  
Ke Wang ◽  
Deyong Chen ◽  
Beiyuan Fan ◽  
Ying Xu ◽  
...  
Keyword(s):  
Single Cells ◽  
Membrane Capacitance ◽  
Impedance Cytometry ◽  
Specific Membrane

scholarly journals Simultaneous Characterization of Instantaneous Young’s Modulus and Specific Membrane Capacitance of Single Cells Using a Microfluidic System

Sensors ◽  
2015 ◽  
Vol 15 (2) ◽  
pp. 2763-2773 ◽  
Author(s):  
Yang Zhao ◽  
Deyong Chen ◽  
Yana Luo ◽  
Feng Chen ◽  
Xiaoting Zhao ◽  
...  
Keyword(s):  
Young’S Modulus ◽  
Young's Modulus ◽  
Single Cells ◽  
Microfluidic System ◽  
Membrane Capacitance ◽  
Specific Membrane

Crossing constriction channel-based microfluidic cytometry capable of electrically phenotyping large populations of single cells

The Analyst ◽  
2019 ◽  
Vol 144 (3) ◽  
pp. 1008-1015 ◽  
Author(s):  
Yi Zhang ◽  
Yang Zhao ◽  
Deyong Chen ◽  
Ke Wang ◽  
Yuanchen Wei ◽  
...  
Keyword(s):  
High Throughput ◽  
Single Cells ◽  
Microfluidic System ◽  
Membrane Capacitance ◽  
Large Populations ◽  
Specific Membrane

This paper presents a crossing constriction channel-based microfluidic system for high-throughput characterization of specific membrane capacitance (Csm) and cytoplasm conductivity (σcy) of single cells.

scholarly journals Membrane capacitance of thousands of single white blood cells

2017 ◽  
Vol 14 (137) ◽  
pp. 20170717 ◽  
Author(s):  
Ke Wang ◽  
Chun-Chieh Chang ◽  
Tzu-Keng Chiu ◽  
Xiaoting Zhao ◽  
Deyong Chen ◽  
...  
Keyword(s):  
Blood Cells ◽  
Single Cells ◽  
Statistical Significance ◽  
Gradient Centrifugation ◽  
White Blood Cells ◽  
Membrane Capacitance ◽  
Label Free ◽  
Specific Membrane ◽  
Total Membrane

As label-free biomarkers, the electrical properties of single cells are widely used for cell type classification and cellular status evaluation. However, as intrinsic cellular electrical markers, previously reported membrane capacitances (e.g. specific membrane capacitance C spec and total membrane capacitance C mem ) of white blood cells were derived from tens of single cells, lacking statistical significance due to low cell numbers. In this study, white blood cells were first separated into granulocytes and lymphocytes by density gradient centrifugation and were then aspirated through a microfluidic constriction channel to characterize both C spec and C mem . Thousands of granulocytes ( n cell = 3327) and lymphocytes ( n cell = 3302) from 10 healthy blood donors were characterized, resulting in C spec values of 1.95 ± 0.22 µF cm −2 versus 2.39 ± 0.39 µF cm −2 and C mem values of 6.81 ± 1.09 pF versus 4.63 ± 0.57 pF. Statistically significant differences between granulocytes and lymphocytes were located for both C spec and C mem . In addition, neural network-based pattern recognition was used to classify white blood cells, producing successful classification rates of 78.1% for C spec and 91.3% for C mem , respectively. These results indicate that as intrinsic bioelectrical markers, membrane capacitances may contribute to the classification of white blood cells.

scholarly journals Direct Measurement of Specific Membrane Capacitance in Neurons

2000 ◽  
Vol 79 (1) ◽  
pp. 314-320 ◽  
Author(s):  
Luc J. Gentet ◽  
Greg J. Stuart ◽  
John D. Clements
Keyword(s):  
Direct Measurement ◽  
Membrane Capacitance ◽  
Specific Membrane
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