Quantum dots’ size matters for balancing their quantity and quality in label materials to improve lateral flow immunoassay performance for C-Reactive protein determination

We report a new approach to synthesize nanozyme with low consumption of precious Pt-precursor and high peroxidase-mimicking activity. The synthesis includes the formation of gold nanoparticles (Au NPs), the overgrowth of a silver layer over Au NPs (Au@Ag NPs), and the galvanic replacement of Ag with PtCl62- leading to the formation of Au@Ag–Pt NPs with uniformly deposited catalytic Pt sites. The reported approach facilitates up to 20-times lower consumption of Pt precursor. The use of Au@Ag-Pt NPs as the catalytic label in lateral flow immunoassay results in a 65-fold lower limit of detection (15 pg/mL in serum).

Download Full-text

POINT-OF-CARE TEST FOR C-REACTIVE PROTEIN BY A FLUORESCENCE-BASED LATERAL FLOW IMMUNOASSAY

Instrumentation Science & Technology ◽

10.1080/10739149.2014.930877 ◽

2014 ◽

Vol 42 (6) ◽

pp. 635-645 ◽

Cited By ~ 9

Author(s):

Yongbin Gu ◽

Yongliang Yang ◽

Jing Zhang ◽

ShengXiang Ge ◽

Zhanghong Tang ◽

...

Keyword(s):

Point Of Care ◽

Lateral Flow ◽

Lateral Flow Immunoassay ◽

C Reactive Protein ◽

Reactive Protein ◽

Point Of Care Test

Download Full-text

Dual-Quantum-Dots-Labeled Lateral Flow Strip Rapidly Quantifies Procalcitonin and C-reactive Protein

Nanoscale Research Letters ◽

10.1186/s11671-016-1383-z ◽

2016 ◽

Vol 11 (1) ◽

Cited By ~ 19

Author(s):

XiaoPing Qi ◽

YunYe Huang ◽

ZhongShi Lin ◽

Liang Xu ◽

Hao Yu

Keyword(s):

Quantum Dots ◽

Lateral Flow ◽

C Reactive Protein ◽

Lateral Flow Strip ◽

Reactive Protein ◽

Dual Quantum

Download Full-text

A facile one-step gold nanoparticles enhancement based on sequential patterned lateral flow immunoassay device for C-reactive protein detection

Sensors and Actuators B Chemical ◽

10.1016/j.snb.2020.129241 ◽

2021 ◽

Vol 329 ◽

pp. 129241

Author(s):

Yosita Panraksa ◽

Amara Apilux ◽

Sakda Jampasa ◽

Songchan Puthong ◽

Charles S. Henry ◽

...

Keyword(s):

Gold Nanoparticles ◽

Protein Detection ◽

Lateral Flow ◽

Lateral Flow Immunoassay ◽

C Reactive Protein ◽

Reactive Protein ◽

One Step

Download Full-text

Silanized quantum dots as labels in lateral flow test strips for C-reactive protein

Analytical Letters ◽

10.1080/00032719.2019.1574302 ◽

2019 ◽

Vol 52 (12) ◽

pp. 1874-1887 ◽

Cited By ~ 3

Author(s):

Aleksandr M. Sobolev ◽

Nadezhda A. Byzova ◽

Irina Yu. Goryacheva ◽

Anatoly V. Zherdev

Keyword(s):

Quantum Dots ◽

Lateral Flow ◽

C Reactive Protein ◽

Test Strips ◽

Reactive Protein ◽

Flow Test ◽

Lateral Flow Test

Download Full-text

Peroxidase-Mimicking Nanozyme with Surface-Dispersed Pt Atoms as the Label for the Lateral Flow Immunoassay of C-Reactive Protein

10.26434/chemrxiv.14171249 ◽

2021 ◽

Author(s):

Vasily Panferov ◽

Nadezhda A. Byzova ◽

Anatoly V. Zherdev ◽

Boris B. Dzantiev

Keyword(s):

Limit Of Detection ◽

Lateral Flow ◽

Lateral Flow Immunoassay ◽

Silver Layer ◽

Galvanic Replacement ◽

C Reactive Protein ◽

Ag Nps ◽

Au Nps ◽

New Approach ◽

Reactive Protein

We report a new approach to synthesize nanozyme with low consumption of precious Pt-precursor and high peroxidase-mimicking activity. The synthesis includes the formation of gold nanoparticles (Au NPs), the overgrowth of a silver layer over Au NPs (Au@Ag NPs), and the galvanic replacement of Ag with PtCl62- leading to the formation of Au@Ag–Pt NPs with uniformly deposited catalytic Pt sites. The reported approach facilitates up to 20-times lower consumption of Pt precursor. The use of Au@Ag-Pt NPs as the catalytic label in lateral flow immunoassay results in a 65-fold lower limit of detection (15 pg/mL in serum).

Download Full-text