scholarly journals Peroxidase-Mimicking Nanozyme with Surface-Dispersed Pt Atoms as the Label for the Lateral Flow Immunoassay of C-Reactive Protein

2021 ◽  
Author(s):  
Vasily Panferov ◽  
Nadezhda A. Byzova ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Limit Of Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Silver Layer ◽  
Galvanic Replacement ◽  
C Reactive Protein ◽  
Ag Nps ◽  
Au Nps ◽  
New Approach ◽  
Reactive Protein

We report a new approach to synthesize nanozyme with low consumption of precious Pt-precursor and high peroxidase-mimicking activity. The synthesis includes the formation of gold nanoparticles (Au NPs), the overgrowth of a silver layer over Au NPs (Au@Ag NPs), and the galvanic replacement of Ag with PtCl<sub>6</sub><sup>2-</sup> leading to the formation of Au@Ag–Pt NPs with uniformly deposited catalytic Pt sites. The reported approach facilitates up to 20-times lower consumption of Pt precursor. The use of Au@Ag-Pt NPs as the catalytic label in lateral flow immunoassay results in a 65-fold lower limit of detection (15 pg/mL in serum).

scholarly journals Peroxidase-Mimicking Nanozyme with Surface-Dispersed Pt Atoms as the Label for the Lateral Flow Immunoassay of C-Reactive Protein

2021 ◽  
Author(s):  
Vasily Panferov ◽  
Nadezhda A. Byzova ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Limit Of Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Silver Layer ◽  
Galvanic Replacement ◽  
C Reactive Protein ◽  
Ag Nps ◽  
Au Nps ◽  
New Approach ◽  
Reactive Protein

We report a new approach to synthesize nanozyme with low consumption of precious Pt-precursor and high peroxidase-mimicking activity. The synthesis includes the formation of gold nanoparticles (Au NPs), the overgrowth of a silver layer over Au NPs (Au@Ag NPs), and the galvanic replacement of Ag with PtCl<sub>6</sub><sup>2-</sup> leading to the formation of Au@Ag–Pt NPs with uniformly deposited catalytic Pt sites. The reported approach facilitates up to 20-times lower consumption of Pt precursor. The use of Au@Ag-Pt NPs as the catalytic label in lateral flow immunoassay results in a 65-fold lower limit of detection (15 pg/mL in serum).

scholarly journals Comparative Study of In Situ Techniques to Enlarge Gold Nanoparticles for Highly Sensitive Lateral Flow Immunoassay of SARS-CoV-2

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 229
Author(s):  
Vasily G. Panferov ◽  
Nadezhda A. Byzova ◽  
Sergey F. Biketov ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Limit Of Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Silver Layer ◽  
Galvanic Replacement ◽  
Silver Enhancement ◽  
Ag Nps ◽  
Au Nps ◽  
Highly Sensitive

Three techniques were compared for lowering the limit of detection (LOD) of the lateral flow immunoassay (LFIA) of the receptor-binding domain of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) based on the post-assay in situ enlargement of Au nanoparticles (Au NPs) on a test strip. Silver enhancement (growth of a silver layer over Au NPs—Au@Ag NPs) and gold enhancement (growth of a gold layer over Au NPs) techniques and the novel technique of galvanic replacement of Ag by Au in Au@Ag NPs causing the formation of Au@Ag-Au NPs were performed. All the enhancements were performed on-site after completion of the conventional LFIA and maintained equipment-free assay. The assays demonstrated lowering of LODs in the following rows: 488 pg/mL (conventional LFIA with Au NPs), 61 pg/mL (silver enhancement), 8 pg/mL (galvanic replacement), and 1 pg/mL (gold enhancement). Using gold enhancement as the optimal technique, the maximal dilution of inactivated SARS-CoV-2-containing samples increased 500 times. The developed LFIA provided highly sensitive and rapid (8 min) point-of-need testing.

Peroxidase-mimicking nanozyme with surface-dispersed Pt atoms for the colorimetric lateral flow immunoassay of C-reactive protein

2021 ◽  
Vol 188 (9) ◽  
Author(s):  
Vasily G. Panferov ◽  
Nadezhda A. Byzova ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
C Reactive Protein ◽  
Reactive Protein

POINT-OF-CARE TEST FOR C-REACTIVE PROTEIN BY A FLUORESCENCE-BASED LATERAL FLOW IMMUNOASSAY

2014 ◽  
Vol 42 (6) ◽  
pp. 635-645 ◽  
Author(s):  
Yongbin Gu ◽  
Yongliang Yang ◽  
Jing Zhang ◽  
ShengXiang Ge ◽  
Zhanghong Tang ◽  
...  
Keyword(s):  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Point Of Care Test

A facile one-step gold nanoparticles enhancement based on sequential patterned lateral flow immunoassay device for C-reactive protein detection

2021 ◽  
Vol 329 ◽  
pp. 129241
Author(s):  
Yosita Panraksa ◽  
Amara Apilux ◽  
Sakda Jampasa ◽  
Songchan Puthong ◽  
Charles S. Henry ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Protein Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
One Step

scholarly journals Development of a gold-nanorod-based lateral flow immunoassay for a fast and dual-modal detection of C-reactive protein in clinical plasma samples

RSC Advances ◽  
2021 ◽  
Vol 11 (45) ◽  
pp. 28388-28394
Author(s):  
Renzhu Pang ◽  
Qunyan Zhu ◽  
Jia Wei ◽  
Yaoqi Wang ◽  
Fengqin Xu ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Gold Nanorod ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection ◽  
Plasma Samples ◽  
C Reactive Protein ◽  
Reactive Protein

A gold-nanorod-based lateral flow immunoassay for rapid and quantitative detection of CRP by simultaneously monitoring the colorimetric and temperature signals.

scholarly journals Siphon-Controlled Automation on a Lab-on-a-Disc Using Event-Triggered Dissolvable Film Valves

Biosensors ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 73
Author(s):  
Brian D. Henderson ◽  
David J. Kinahan ◽  
Jeanne Rio ◽  
Rohit Mishra ◽  
Damien King ◽  
...  
Keyword(s):  
Risk Factor ◽  
Limit Of Detection ◽  
C Reactive Protein ◽  
Cvd Risk ◽  
Low Resource Settings ◽  
Low Resource ◽  
Reactive Protein ◽  
Limit Of Quantitation ◽  
Chemiluminescent Immunoassay ◽  
Event Triggered

Within microfluidic technologies, the centrifugal microfluidic “Lab-on-a-Disc” (LoaD) platform offers great potential for use at the PoC and in low-resource settings due to its robustness and the ability to port and miniaturize ‘wet bench’ laboratory protocols. We present the combination of ‘event-triggered dissolvable film valves’ with a centrifugo-pneumatic siphon structure to enable control and timing, through changes in disc spin-speed, of the release and incubations of eight samples/reagents/wash buffers. Based on these microfluidic techniques, we integrated and automated a chemiluminescent immunoassay for detection of the CVD risk factor marker C-reactive protein displaying a limit of detection (LOD) of 44.87 ng mL−1 and limit of quantitation (LoQ) of 135.87 ng mL−1.

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