Real-time monitoring of single-cell secretion with a high-throughput nanoplasmonic microarray

Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.

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High-Throughput Single-Cell Real-Time Quantitative PCR Analysis

Methods in Molecular Biology - Single Cell Methods ◽

10.1007/978-1-4939-9240-9_11 ◽

2019 ◽

pp. 177-183

Author(s):

Liora Haim-Vilmovsky

Keyword(s):

Single Cell ◽

Real Time ◽

Quantitative Pcr ◽

High Throughput ◽

Pcr Analysis ◽

Real Time Quantitative Pcr

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High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry

Nature Communications ◽

10.1038/s41467-019-08370-3 ◽

2019 ◽

Vol 10 (1) ◽

Cited By ~ 22

Author(s):

Bob Fregin ◽

Fabian Czerwinski ◽

Doreen Biedenweg ◽

Salvatore Girardo ◽

Stefan Gross ◽

...

Keyword(s):

Single Cell ◽

Real Time ◽

High Throughput ◽

Complex Samples ◽

Cell Rheology ◽

Deformability Cytometry

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High-Throughput Cell Death Assays with Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling (SPARKL)

STAR Protocols ◽

10.1016/j.xpro.2020.100034 ◽

2020 ◽

Vol 1 (1) ◽

pp. 100034

Author(s):

Jesse D. Gelles ◽

Jerry E. Chipuk

Keyword(s):

Cell Death ◽

Single Cell ◽

Real Time ◽

High Throughput ◽

Population Level

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High-throughput fermentation screening for the yeast Yarrowia lipolytica with real-time monitoring of biomass and lipid production

Microbial Cell Factories ◽

10.1186/s12934-016-0546-z ◽

2016 ◽

Vol 15 (1) ◽

Cited By ~ 32

Author(s):

Alexandre Back ◽

Tristan Rossignol ◽

François Krier ◽

Jean-Marc Nicaud ◽

Pascal Dhulster

Keyword(s):

Real Time ◽

Yarrowia Lipolytica ◽

High Throughput ◽

Lipid Production ◽

Real Time Monitoring ◽

Yeast Yarrowia Lipolytica

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Inertial-Force-Assisted, High-Throughput, Droplet-Free, Single-Cell Sampling Coupled with ICP-MS for Real-Time Cell Analysis

Analytical Chemistry ◽

10.1021/acs.analchem.0c00376 ◽

2020 ◽

Vol 92 (9) ◽

pp. 6604-6612 ◽

Cited By ~ 1

Author(s):

Xuan Zhang ◽

Xing Wei ◽

Xue Men ◽

Ze Jiang ◽

Wen-Qi Ye ◽

...

Keyword(s):

Single Cell ◽

Real Time ◽

High Throughput ◽

Inertial Force ◽

Cell Analysis ◽

Icp Ms

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Real-Time Monitoring of Luciferase-Tagged Cronobacter sakazakii in Reconstituted Infant Milk Formula

Journal of Food Protection ◽

10.4315/0362-028x.jfp-10-403 ◽

2011 ◽

Vol 74 (4) ◽

pp. 573-579 ◽

Cited By ~ 11

Author(s):

RUTH MORRISSEY ◽

MÁIRE BEGLEY ◽

SATORU OSHIMA ◽

MARY REA ◽

R. PAUL ROSS ◽

...

Keyword(s):

Real Time ◽

High Throughput ◽

Skim Milk ◽

Luria Bertani ◽

Milk Formula ◽

Cronobacter Sakazakii ◽

Real Time Monitoring ◽

Luria Bertani Broth ◽

Infant Milk Formula

The aim of this study was to examine the potential of using a lux-tagged Cronobacter sakazakii strain to monitor growth of the bacterium in various liquids. C. sakazakii was transformed with plasmid p16Slux, and integration of the plasmid at the desired site on the chromosome was confirmed by PCR. The growth of the lux-tagged strain was similar to that of the non–lux-tagged strain, and the integrated plasmid was stable when cells were cultured in the absence of antibiotic. Growth of the lux-tagged strain was monitored in real time in Luria-Bertani broth, skim milk, and infant milk formula by using both the Luminoskan luminometer and the Xenogen IVIS imager. Bioluminescence could be detected when the lux-tagged strain was cocultured with other bacteria. The effect of monocaprylin and nisin on the growth of C. sakazakii in milk was monitored by measuring bioluminescence. In conclusion, growth of a lux-tagged C. sakazakii can be monitored in real time in both clear and opaque liquids by measuring bioluminescence. lux-tagged C. sakazakii strains could be potentially used in high-throughput assays to monitor the effects of various infant milk formula compositions on growth of the bacterium.

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