One-step immunoassay for food allergens based on screened mimotopes from autodisplayed FV-antibody library

2022 ◽  
pp. 113976
Author(s):  
Jeong Soo Sung ◽  
Ji-Hong Bong ◽  
Soo Jeong Lee ◽  
Jaeyong Jung ◽  
Min-Jung Kang ◽  
...  
Keyword(s):  
Food Allergens ◽  
Antibody Library ◽  
Fv Antibody ◽  
One Step

Fluorescein and Rhodamine B-Binding Domains from Autodisplayed Fv-Antibody Library

2021 ◽  
Author(s):  
Jaeyong Jung ◽  
Ji-Hong Bong ◽  
Jeong Soo Sung ◽  
Soo Jeong Lee ◽  
Misu Lee ◽  
...  
Keyword(s):  
Rhodamine B ◽  
Antibody Library ◽  
Binding Domains ◽  
Fv Antibody

Hexa-histidine tag as a novel alternative for one-step direct labelling of a single-chain Fv antibody fragment with 99mTc

2001 ◽  
Vol 22 (10) ◽  
pp. 1089-1094 ◽  
Author(s):  
G. J. PIMENTEL ◽  
J. E. VÁZQUEZ ◽  
W. QUESADA ◽  
Y. FELIPE ◽  
C. CARDERÓN ◽  
...  
Keyword(s):  
Antibody Fragment ◽  
Single Chain ◽  
Single Chain Fv ◽  
Fv Antibody ◽  
Direct Labelling ◽  
One Step ◽  
Single Chain Fv Antibody ◽  
Histidine Tag

One-Step Detection of Aflatoxin-B1 Using scFv-Alkaline Phosphatase-Fusion Selected from Human Phage Display Antibody Library

2011 ◽  
Vol 49 (3) ◽  
pp. 240-249 ◽  
Author(s):  
Kuntalee Rangnoi ◽  
Nanthnit Jaruseranee ◽  
Richard O’Kennedy ◽  
Potjamas Pansri ◽  
Montarop Yamabhai
Keyword(s):  
Alkaline Phosphatase ◽  
Phage Display ◽  
Aflatoxin B1 ◽  
Antibody Library ◽  
Step Detection ◽  
One Step ◽  
Phage Display Antibody

scholarly journals Phage-display antibody detection of Chlamydia trachomatis-associated antigens

Microbiology ◽  
2002 ◽  
Vol 148 (2) ◽  
pp. 443-451 ◽  
Author(s):  
Erika A Lindquist ◽  
James D Marks ◽  
Betsy J Kleba ◽  
Richard S Stephens
Keyword(s):  
Chlamydia Trachomatis ◽  
Phage Display ◽  
Antibody Detection ◽  
Antibody Library ◽  
Single Chain ◽  
Dot Blot ◽  
Single Chain Fv ◽  
Cell Components ◽  
Fv Antibody ◽  
Selection Of

A phage-displayed human single-chain Fv antibody library (6·7×109 members) was used to select probes specific to components associated with the surface of Chlamydia trachomatis elementary bodies (EBs). Each of 15 antibodies was characterized by ELISA, dot-blot, immunoblot and immunocytochemistry, resulting in the identification of several new chlamydial components associated with the surface of EBs. In addition, six antibodies were specific for host-cell components associated with the surface of EBs. While phage display has been used effectively to produce specific antibodies for purified components, these data show that this technology is suitable for selection of specific probes from complex antigens such as the surface of a microbial pathogen.

An Interactive Minicomputer Program for the Indexing and Simulation of Electron Diffraction Patterns

1976 ◽  
Vol 34 ◽  
pp. 542-543
Author(s):  
R.P. Goehner ◽  
W.T. Hatfield ◽  
Prakash Rao
Keyword(s):  
Electron Diffraction ◽  
Real Time ◽  
Pattern Analysis ◽  
Flow Diagram ◽  
Hard Copy ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Transmission Electron ◽  
One Step ◽  
Diffraction Patterns

Computer programs are now available in various laboratories for the indexing and simulation of transmission electron diffraction patterns. Although these programs address themselves to the solution of various aspects of the indexing and simulation process, the ultimate goal is to perform real time diffraction pattern analysis directly off of the imaging screen of the transmission electron microscope. The program to be described in this paper represents one step prior to real time analysis. It involves the combination of two programs, described in an earlier paper(l), into a single program for use on an interactive basis with a minicomputer. In our case, the minicomputer is an INTERDATA 70 equipped with a Tektronix 4010-1 graphical display terminal and hard copy unit.A simplified flow diagram of the combined program, written in Fortran IV, is shown in Figure 1. It consists of two programs INDEX and TEDP which index and simulate electron diffraction patterns respectively. The user has the option of choosing either the indexing or simulating aspects of the combined program.

Nutrient-regulated gene expression in eukaryotes

2006 ◽  
Vol 73 ◽  
pp. 85-96 ◽  
Author(s):  
Richard J. Reece ◽  
Laila Beynon ◽  
Stacey Holden ◽  
Amanda D. Hughes ◽  
Karine Rébora ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcriptional Control ◽  
Direct Interaction ◽  
Signalling Pathways ◽  
A Cell ◽  
One Step ◽  
Higher Eukaryotes ◽  
Regulated Gene Expression

The recognition of changes in environmental conditions, and the ability to adapt to these changes, is essential for the viability of cells. There are numerous well characterized systems by which the presence or absence of an individual metabolite may be recognized by a cell. However, the recognition of a metabolite is just one step in a process that often results in changes in the expression of whole sets of genes required to respond to that metabolite. In higher eukaryotes, the signalling pathway between metabolite recognition and transcriptional control can be complex. Recent evidence from the relatively simple eukaryote yeast suggests that complex signalling pathways may be circumvented through the direct interaction between individual metabolites and regulators of RNA polymerase II-mediated transcription. Biochemical and structural analyses are beginning to unravel these elegant genetic control elements.

CAD: One Step Forward, One Step Back

2010 ◽  
Vol 43 (18) ◽  
pp. 16
Author(s):  
MATTHEW R.G. TAYLOR
Keyword(s):  
One Step
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