Analysis of insulin amyloid fibrils by Raman spectroscopy

2007 ◽  
Vol 128 (2-3) ◽  
pp. 150-155 ◽  
Author(s):  
Corasi Ortiz ◽  
Dongmao Zhang ◽  
Alexander E. Ribbe ◽  
Yong Xie ◽  
Dor Ben-Amotz
The Analyst ◽  
2015 ◽  
Vol 140 (15) ◽  
pp. 4967-4980 ◽  
Author(s):  
Dmitry Kurouski ◽  
Richard P. Van Duyne ◽  
Igor K. Lednev

Applications of Raman spectroscopy, a label-free non-destructive technique, for the structural characterization of amyloidogenic proteins, prefibrilar oligomers, and mature fibrils.


2012 ◽  
Vol 116 (27) ◽  
pp. 7926-7930 ◽  
Author(s):  
Victor Shashilov ◽  
Ming Xu ◽  
Natallia Makarava ◽  
Regina Savtchenko ◽  
Ilia V. Baskakov ◽  
...  

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3556
Author(s):  
Martynas Talaikis ◽  
Simona Strazdaitė ◽  
Mantas Žiaunys ◽  
Gediminas Niaura

Several neurodegenerative diseases, like Alzheimer’s and Parkinson’s are linked with protein aggregation into amyloid fibrils. Conformational changes of native protein into the β-sheet structure are associated with a significant change in the vibrational spectrum. This is especially true for amide bands which are inherently sensitive to the secondary structure of a protein. Raman amide bands are greatly intensified under resonance conditions, in the UV spectral range, allowing for the selective probing of the peptide backbone. In this work, we examine parallel β-sheet forming GGVVIA, the C-terminus segment of amyloid-β peptide, using UV–Vis, FTIR, and multiwavelength Raman spectroscopy. We find that amide bands are enhanced far from the expected UV range, i.e., at 442 nm. A reasonable two-fold relative intensity increase is observed for amide II mode (normalized according to the δCH2/δCH3 vibration) while comparing 442 and 633 nm excitations; an increase in relative intensity of other amide bands was also visible. The observed relative intensification of amide II, amide S, and amide III modes in the Raman spectrum recorded at 442 nm comparing with longer wavelength (633/785/830 nm) excited spectra allows unambiguous identification of amide bands in the complex Raman spectra of peptides and proteins containing the β-sheet structure.


Small ◽  
2015 ◽  
Vol 11 (33) ◽  
pp. 4130-4130
Author(s):  
Corianne C. vandenAkker ◽  
Tanja Deckert-Gaudig ◽  
Michael Schleeger ◽  
Krassimir P. Velikov ◽  
Volker Deckert ◽  
...  

2017 ◽  
Vol 199 (2) ◽  
pp. 140-152 ◽  
Author(s):  
Nikolaos N. Louros ◽  
Paraskevi L. Tsiolaki ◽  
Fotis A. Baltoumas ◽  
Georgios D. Chryssikos ◽  
Vassilis Gionis ◽  
...  

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