Regional Assessment of Carbon Pool Response to Intensive Silvicultural Practices in Loblolly Pine Plantations

Tree plantations represent an important component of the global carbon (C) cycle and are expected to increase in prevalence during the 21st century. We examined how silvicultural approaches that optimize economic returns in loblolly pine (Pinus taeda L.) plantations affected the accumulation of C in pools of vegetation, detritus, and mineral soil up to 100 cm across the loblolly pine’s natural range in the southeastern United States. Comparisons of silvicultural treatments included competing vegetation or ‘weed’ control, fertilization, thinning, and varying intensities of silvicultural treatment for 106 experimental plantations and 322 plots. The average age of the sampled plantations was 17 years, and the C stored in vegetation (pine and understory) averaged 82.1 ± 3.0 (±std. error) Mg C ha−1, and 14.3 ± 0.6 Mg C ha−1 in detrital pools (soil organic layers, coarse-woody debris, and soil detritus). Mineral soil C (0–100 cm) averaged 79.8 ± 4.6 Mg C ha−1 across sites. For management effects, thinning reduced vegetation by 35.5 ± 1.2 Mg C ha−1 for all treatment combinations. Weed control and fertilization increased vegetation between 2.3 and 5.7 Mg C ha−1 across treatment combinations, with high intensity silvicultural applications producing greater vegetation C than low intensity (increase of 21.4 ± 1.7 Mg C ha−1). Detrital C pools were negatively affected by thinning where either fertilization or weed control were also applied, and were increased with management intensity. Mineral soil C did not respond to any silvicultural treatments. From these data, we constructed regression models that summarized the C accumulation in detritus and detritus + vegetation in response to independent variables commonly monitored by plantation managers (site index (SI), trees per hectare (TPH) and plantation age (AGE)). The C stored in detritus and vegetation increased on average with AGE and both models included SI and TPH. The detritus model explained less variance (adj. R2 = 0.29) than the detritus + vegetation model (adj. R2 = 0.87). A general recommendation for managers looking to maximize C storage would be to maintain a high TPH and increase SI, with SI manipulation having a greater relative effect. From the model, we predict that a plantation managed to achieve the average upper third SI (26.8) within our observations, and planted at 1500 TPH, could accumulate ~85 Mg C ha−1 by 12 years of age in detritus and vegetation, an amount greater than the region’s average mineral soil C pool. Notably, SI can be increased using both genetic and silviculture technologies.

Properties of the PVA-VAVTD KOH Blend as a Gel Polymer Electrolyte for Zinc Batteries

Rechargeable zinc-air batteries are promising for energy storage and portable electronic applications because of their good safety, high energy density, material abundance, low cost, and environmental friendliness. A series of alkaline gel polymer electrolytes formed from polyvinyl alcohol (PVA) and different amounts of terpolymer composed of butyl acrylate, vinyl acetate, and vinyl neodecanoate (VAVTD) was synthesized applying a solution casting technique. The thin films were doped with KOH 12M, providing a higher amount of water and free ions inside the electrolyte matrix. The inclusion of VAVTD together with the PVA polymer improved several of the electrical properties of the PVA-based gel polymer electrolytes (GPEs). X-ray diffraction (XRD), thermogravimetric analysis (TGA), and attenuated total reflectance- Fourier-transform infrared spectroscopy (ATR-FTIR) tests, confirming that PVA chains rearrange depending on the VAVTD content and improving the amorphous region. The most conducting electrolyte film was the test specimen 1:4 (PVA-VAVTD) soaked in KOH solution, reaching a conductivity of 0.019 S/cm at room temperature. The temperature dependence of the conductivity agrees with the Arrhenius equation and activation energy of ~0.077 eV resulted, depending on the electrolyte composition. In addition, the cyclic voltammetry study showed a current intensity increase at higher VAVTD content, reaching values of 310 mA. Finally, these gel polymer electrolytes were tested in Zn–air batteries, obtaining capacities of 165 mAh and 195 mAh for PVA-T4 and PVA-T5 sunk in KOH, respectively, at a discharge current of −5 mA.

Zeptomolar detection of 4-aminothiophenol by SERS using silver nanodendrites decorated with gold nanoparticles

In the present work we report a simple, fast, reproducible and cheap methodology for SERS substrate fabrication of silver dendritic nanostructures (prepared by electrodeposition) decorated with gold nanospheres by electrophoretic deposition. This is the first report where a metal dendritic nanostructure has been decorated with another type of metal nanoparticles by this technique. The decorated nanostructures were used directly as SERS substrate using 4-aminothiophenol (4-ATP) as analyte. The objective of the decoration is to create more hot-spots in order to detect the analyte in a lower concentration. Decorated nanodendrites had a detection limit one million times lower than bare silver nanodendrites and all the substrates showed an increase in the Raman intensity at concentrations below 1 nM; because this concentration corresponds to the threshold for the formation of a monolayer resulting in a triple mechanism of intensity increase, namely electric field, chemical factor and hot-spots. 4-ATP was detected in zeptomolar concentration, which is below 1 ppq, corresponding to an analytical enhancement factor (AEF) in the order of 1015.

Interaction between Multiple Current Sheets and a Shock Wave: 2D Hybrid Kinetic Simulations

Particle acceleration behind a shock wave due to interactions between magnetic islands in the heliosphere has attracted attention in recent years. The downstream acceleration may yield a continuous increase of particle flux downstream of the shock wave. Although it is not obvious how the downstream magnetic islands are produced, it has been suggested that current sheets are involved in the generation of magnetic islands due to their interaction with a shock wave. We perform 2D hybrid kinetic simulations to investigate the interaction between multiple current sheets and a shock wave. In the simulation, current sheets are compressed by the shock wave and a tearing instability develops at the compressed current sheets downstream of the shock. As the result of this instability, the electromagnetic fields become turbulent and magnetic islands form well downstream of the shock wave. We find a “post-cursor” region in which the downstream flow speed normal to the shock wave in the downstream rest frame is decelerated to ∼ 1V A immediately behind the shock wave, where V A is the upstream Alfvén speed. The flow speed then gradually decelerates to 0 accompanied by the development of the tearing instability. We also observe an efficient production of energetic particles above 100 E 0 during the development of the instability some distance downstream of the shock wave, where E 0 = m p V A 2 and m p is the proton mass. This feature corresponds to Voyager observations showing that the anomalous cosmic-ray intensity increase begins some distance downstream of the heliospheric termination shock.

Identification of bacteria by poly-aromatic hydrocarbons biosensors

Human health is consistently threatened by different species of pathogenic bacteria. To fight the spread of diseases, it is important to develop rapid methods for bacterial identification. Over the years, different kinds of biosensors were developed for this cause. Another environmental risk are polyaromatic hydrocarbons (PAHs) that may be emitted from industrial facilities and pollute environmental water and soil. One of the methods for their purification is conducted by the addition of bacteria that can degrade the PAHs, while the bacteria itself can be filtrated at the end of the process. Although many studies reported monitoring of the PAHs degradation by fluorescence, not much attention was dedicated to studying the influence of the PAHs on the intrinsic fluorescence of the degrading bacteria. In this work, we apply synchronous fluorescence (SF) measurements to study the ability of the 5 PAHs: 9Antracene carboxylic acid (9ACA), Pyrene, Perylene, Pentacene, and Chrysene to interact with bacteria and change its fluorescence spectra. We show that upon incubation of each PAH with the bacterium E.coli only the 2 PAHs 9ACA and Perylene cause an intensity decrease in the emission at λ = 300 – 375 nm, which derives from the emission of Tyrosine and Tryptophane (TT). Also, we show that upon incubation of 9ACA and Perylene with 5 different pathogenic bacteria, the intensity increase or decrease in the TT emission is unique to each bacterial species. Based on this observation, we suggest that the PAHs 9ACA and Perylene can be utilized as biosensors for bacterial identification.

The role of cyclonic activity in tropical temperature-rainfall scaling

The attribution of changing intensity of rainfall extremes to global warming is a key challenge of climate research. From a thermodynamic perspective, via the Clausius-Clapeyron relationship, rainfall events are expected to become stronger due to the increased water-holding capacity of a warmer atmosphere. Here, we employ global, 1-hourly temperature and 3-hourly rainfall data to investigate the scaling between temperature and extreme rainfall. Although the Clausius-Clapeyron scaling of +7% rainfall intensity increase per degree warming roughly holds on a global average, we find very heterogeneous spatial patterns. Over tropical oceans, we reveal areas with consistently strong negative scaling (below −40%∘C−1). We show that the negative scaling is due to a robust linear correlation between pre-rainfall cooling of near-surface air temperature and extreme rainfall intensity. We explain this correlation by atmospheric and oceanic dynamics associated with cyclonic activity. Our results emphasize that thermodynamic arguments alone are not enough to attribute changing rainfall extremes to global warming. Circulation dynamics must also be thoroughly considered.

Enhanced fluorescence from semiconductor quantum dot-labelled cells excited at 280 nm

Semiconductor quantum dots (QDs) have significant advantages over more traditional fluorophores used in fluorescence microscopy including reduced photobleaching, long-term photostability and high quantum yields, but due to limitations in light sources and optics, are often excited far from their optimum excitation wavelengths in the deep-UV. Here, we present a quantitative comparison of the excitation of semiconductor QDs at a wavelength of 280 nm, compared to the longer wavelength of 365 nm, within a cellular environment. We report increased fluorescence intensity and enhanced image quality when using 280 nm excitation compared to 365 nm excitation for cell imaging across multiple datasets, with a highest average fluorescence intensity increase of 3.59-fold. We also find no significant increase in photobleaching of QDs associated with 280 nm excitation.

A multi-responsive coumarin–benzothiazole fluorescent probe for selective detection of biological thiols and hydrazine

Biothiols play important roles in various physiological and biological processes, which closely related to many diseases. Hydrazine is widely used in the chemical industry, but it is harmful to humans and animals. Therefore, it is very important to develop a fluorescent probe that can simultaneously detect biological thiols and hydrazine. In this work, a new fluorescent probe (2E,4Z)-2-(benzo[d]thiazol-2-yl)-5-chloro-5-(7-(diethylamino)-2-oxo-2H-chromen-3-yl)penta-2,4-dienenitrile (BCD) was synthesized by integrating coumarin and benzothiazole acetonitrile. Featured with four binding sites and different bonding mechanism between probe with biothiols and hydrazine, this probe exhibited fluorescent turn-on for distinguishing Cys, Hcy, GSH and hydrazine with 760-, 8-, 6- and 637-fold fluorescent intensity increase at 502, 479, 476 and 458 nm, respectively, through different excitation wavelengths. Research on the effect of pH on the fluorescent performance of BCD shows that the probe exhibits superior stability in a weakly alkaline to weakly acidic environment, which will facilitate the detection of biological thiols or hydrazine hydrate by BCD. Selectivity studies have shown that the probe has high specificity to biological thiols and hydrazine, which is of great significance to the application of BCD.

Low Concentration Ethanol Prevents Oxidative Stress- Induced Cardiac Cells Injury Under HyperglycemicConditions by Inhibiting the SAPK/JNK Signaling Pathway and ROS Generation

The purpose of this study was to determine the effects and mechanisms of ethanol on oxidative stress-induced cardiac H9c2 cells mitochondrial injury under hyperglycemic conditions. Under hyperglycemic conditions, ethanol pretreatment (10-100 μM) prevented H2O2-induced mitochondria swelling, as well as decreased cell viability and Respiratory Control Ratio (RCR) in the H9c2 cells. It also prevented TMRE fluorescence intensity loss and DCF fluorescence intensity increase under hyperglycemic conditions. These effects of ethanol were reversed by the SAPK/JNK agonist, anisomycin. Finally, treatment of H9c2 cells with 33mM glucose significantly enhanced Akt and ERK phosphorylation, which was not affected by ethanol. However, ethanol decreased the phosphorylation of SAPK/JNK under hyperglycemic conditions. Collectively, these findings indicate that under hyperglycemic conditions, that ethanol prevents oxidative stress-induced mitochondrial injury in cardiac H9c2 cells by preventing ROS generation via inhibiting the SAPK/JNK signaling pathway.

Light intensity dependence of organic solar cell operation and dominance switching between Shockley–Read–Hall and bimolecular recombination losses

We investigated the variation of current density–voltage (J–V) characteristics of an organic solar cell (OSC) in the dark and at 9 different light intensities ranging from 0.01 to 1 sun of the AM1.5G spectrum. All three conventional parameters, short-circuit currents (Jsc), open-circuit voltage (Voc), and Fill factor (FF), representing OSC performance evolved systematically in response to light intensity increase. Unlike Jsc that showed quasi-linear monotonic increase, Voc and FF showed distinctive non-monotonic variations. To elucidate the origin of such variations, we performed extensive simulation studies including Shockley–Read–Hall (SRH) recombination losses. Simulation results were sensitive to defect densities, and simultaneous agreement to 10 measured J–V curves was possible only with the defect density of $$5 \times 10^{12} {\text{ cm}}^{ - 3}$$ 5 × 10 12 cm - 3 . Based on analyses of simulation results, we were able to separate current losses into SRH- and bimolecular-recombination components and, moreover, identify that the competition between SRH- and bimolecular-loss currents were responsible for the aforementioned variations in Jsc, Voc, and FF. In particular, we verified that apparent demarcation in Voc, and FF variations, which seemed to appear at different light intensities, originated from the same mechanism of dominance switching between recombination losses.