Spectroscopic evidence of tetanus toxin translocation domain bilayer-induced refolding and insertion

2021 ◽  
Author(s):  
P.T. O’Neil ◽  
V. Vasquez-Montes ◽  
L. Swint-Kruse ◽  
M.R. Baldwin ◽  
A.S. Ladokhin
Keyword(s):  
Tetanus Toxin ◽  
Spectroscopic Evidence ◽  
Translocation Domain

Hybrid Tetanus Toxin C Fragment-Diphtheria Toxin Translocation Domain Allows Specific Gene Transfer into PC12 Cells

2002 ◽  
Vol 177 (1) ◽  
pp. 75-87 ◽  
Author(s):  
Shahram Barati ◽  
Fariba Chegini ◽  
Plinio Hurtado ◽  
Robert A. Rush
Keyword(s):  
Gene Transfer ◽  
Pc12 Cells ◽  
Tetanus Toxin ◽  
Diphtheria Toxin ◽  
Specific Gene ◽  
Translocation Domain

Two-Dimensional Crystallization of Tetanus Toxin

1985 ◽  
Vol 43 ◽  
pp. 528-529
Author(s):  
Jeffry A. Reidler ◽  
John P. Robinson
Keyword(s):  
Electron Microscopy ◽  
Tetanus Toxin ◽  
Structural Information ◽  
Three Dimensional ◽  
Two Dimensional ◽  
Membrane Interface ◽  
3D Reconstructions ◽  
Cellular Receptors ◽  
Computer Reconstruction ◽  
2D Crystals

We have prepared two-dimensional (2D) crystals of tetanus toxin using procedures developed by Uzgiris and Kornberg for the directed production of 2D crystals of monoclonal antibodies at an antigen-phospholipid monolayer interface. The tetanus toxin crystals were formed using a small mole fraction of the natural receptor, GT1, incorporated into phosphatidyl choline monolayers. The crystals formed at low concentration overnight. Two dimensional crystals of this type are particularly useful for structure determination using electron microscopy and computer image refinement. Three dimensional (3D) structural information can be derived from these crystals by computer reconstruction of photographs of toxin crystals taken at different tilt angles. Such 3D reconstructions may help elucidate the mechanism of entry of the enzymatic subunit of toxins into cells, particularly since these crystals form directly on a membrane interface at similar concentrations of ganglioside GT1 to the natural cellular receptors.

NADH in Solution: A Single State

2019 ◽  
Author(s):  
Joachim Hönes ◽  
Julia Wack ◽  
Katja Schmitz
Keyword(s):  
Aqueous Solution ◽  
Base Stacking ◽  
Spectroscopic Evidence ◽  
Single State

<div>Spectroscopic evidence is presented to show that NADH in aqueous solution does not exist as a folded/unfolded equilibrium but as a single state. The molecule is folded but without base stacking between dihydronicotinamide and adenine.<br></div>

scholarly journals Dihydroflavonol and Flavonol Derivatives from Macaranga Recurvata

2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701
Author(s):  
Mulyadi Tanjung ◽  
Euis H. Hakim ◽  
Elfahmi ◽  
Jalifah Latip ◽  
Yana M. Syah
Keyword(s):  
Strong Activity ◽  
Spectroscopic Evidence ◽  
New Compounds ◽  
Flavonol Derivatives

Two new dihydroflavonol derivatives, macarecurvatins A and B, have been isolated from the leaves of Macaranga recurvata(Euphorbiaceaae), along with the known compounds diisoprenylaromadendrin, glyasperin A and broussoflavonol F. The structures of the new compounds were determined on the basis of spectroscopic evidence. Upon cytotoxic evaluation against P-388 cells, macarecurvatin B showed strong activity with an IC50 of 0.83 μM.

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