scholarly journals Harnessing shear stress preconditioning to improve cell viability in 3D post-printed biostructures using extrusion bioprinting

Bioprinting ◽  
2021 ◽  
pp. e00184
Author(s):  
Selwa Boularaoui ◽  
Aya Shanti ◽  
Kamran A. Khan ◽  
Saverio Iacoponi ◽  
Nicolas Christoforou ◽  
...  
Keyword(s):  
Shear Stress ◽  
Cell Viability ◽  
Improve Cell Viability

scholarly journals Rapid Fabrication of Microfluidic Devices for Biological Mimicking: A Survey of Materials and Biocompatibility

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 346
Author(s):  
Hui Ling Ma ◽  
Ana Carolina Urbaczek ◽  
Fayene Zeferino Ribeiro de Souza ◽  
Paulo Augusto Gomes Garrido Carneiro Leão ◽  
Janice Rodrigues Perussi ◽  
...  
Keyword(s):  
Shear Stress ◽  
Cell Viability ◽  
Cellular Response ◽  
Fluid Shear Stress ◽  
Umbilical Vein ◽  
Microfluidic Devices ◽  
In Vitro Assays ◽  
Stress Effect

Microfluidics is an essential technique used in the development of in vitro models for mimicking complex biological systems. The microchip with microfluidic flows offers the precise control of the microenvironment where the cells can grow and structure inside channels to resemble in vivo conditions allowing a proper cellular response investigation. Hence, this study aimed to develop low-cost, simple microchips to simulate the shear stress effect on the human umbilical vein endothelial cells (HUVEC). Differentially from other biological microfluidic devices described in the literature, we used readily available tools like heat-lamination, toner printer, laser cutter and biocompatible double-sided adhesive tapes to bind different layers of materials together, forming a designed composite with a microchannel. In addition, we screened alternative substrates, including polyester-toner, polyester-vinyl, glass, Permanox® and polystyrene to compose the microchips for optimizing cell adhesion, then enabling these microdevices when coupled to a syringe pump, the cells can withstand the fluid shear stress range from 1 to 4 dyne cm2. The cell viability was monitored by acridine orange/ethidium bromide (AO/EB) staining to detect live and dead cells. As a result, our fabrication processes were cost-effective and straightforward. The materials investigated in the assembling of the microchips exhibited good cell viability and biocompatibility, providing a dynamic microenvironment for cell proliferation. Therefore, we suggest that these microchips could be available everywhere, allowing in vitro assays for daily laboratory experiments and further developing the organ-on-a-chip concept.

A bioprinted composite hydrogel with controlled shear stress on cells

2020 ◽  
pp. 095441192097668
Author(s):  
Amirhossein Bakhtiiari ◽  
Rezvan Khorshidi ◽  
Fatemeh Yazdian ◽  
Hamid Rashedi ◽  
Meisam Omidi
Keyword(s):  
Shear Stress ◽  
Cell Viability ◽  
Room Temperature ◽  
Degradation Rate ◽  
Diffusion Rate ◽  
Sol Gel ◽  
Three Dimensional ◽  
Simulation Software ◽  
Sol Gel Transition ◽  
Gel Transition

In recent decades, three dimensional (3D) bio-printing technology has found widespread use in tissue engineering applications. The aim of this study is to scrutinize different parameters of the bioprinter – with the help of simulation software – to print a hydrogel so much so that avoid high amounts of shear stress which is detrimental for cell viability and cell proliferation. Rheology analysis was done on several hydrogels composed of different percentages of components: alginate, collagen, and gelatin. The results have led to the combination of percentages collagen:alginate:gelatin (1:4:8)% as the best condition which makes sol-gel transition at room temperature possible. The results have shown the highest diffusion rate and cell viability for the cross-linked sample with 1.5% CaCl2 for the duration of 1 h. Finally, we have succeeded in printing the hydrogel that is mechanically strong with suitable degradation rate and cell viability.

scholarly journals Cutting-edge design to improve cell viability in osteochondral grafts

2005 ◽  
Vol 13 (8) ◽  
pp. 665-671 ◽  
Author(s):  
J.S. Huntley ◽  
J.M. McBirnie ◽  
A.H. Simpson ◽  
A.C. Hall
Keyword(s):  
Cell Viability ◽  
Cutting Edge ◽  
Osteochondral Grafts ◽  
Improve Cell Viability

MOOD STABILIZERS IMPROVE CELL VIABILITY AND MITOCHONDRIAL MEMBRANE POTENTIAL IN VITRO

2019 ◽  
Vol 29 ◽  
pp. S901-S902
Author(s):  
Pradip Paul ◽  
Rashmitha Nayak ◽  
Ravikumar Nadella ◽  
Somdatta Sen ◽  
Biju Viswanath ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Cell Viability ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Mood Stabilizers ◽  
Improve Cell Viability

Microbial engineering strategies to improve cell viability for biochemical production

2013 ◽  
Vol 31 (6) ◽  
pp. 903-914 ◽  
Author(s):  
Tat-Ming Lo ◽  
Wei Suong Teo ◽  
Hua Ling ◽  
Binbin Chen ◽  
Aram Kang ◽  
...  
Keyword(s):  
Cell Viability ◽  
Biochemical Production ◽  
Improve Cell Viability

An overview of extrusion-based bioprinting with a focus on induced shear stress and its effect on cell viability

Bioprinting ◽  
2020 ◽  
Vol 20 ◽  
pp. e00093 ◽  
Author(s):  
Selwa Boularaoui ◽  
Ghada Al Hussein ◽  
Kamran A. Khan ◽  
Nicolas Christoforou ◽  
Cesare Stefanini
Keyword(s):  
Shear Stress ◽  
Cell Viability

scholarly journals Evaluating Cell Viability Heterogeneity Based on Information Fusion of Multiple Adhesion Strengths

2020 ◽  
Author(s):  
Mingji Wei ◽  
Rongbiao Zhang ◽  
Fei Zhang ◽  
Yecheng Zhang
Keyword(s):  
Shear Stress ◽  
Cell Viability ◽  
Information Fusion ◽  
Single Channel ◽  
Cell Counting ◽  
Parameter Method ◽  
Fusion Method ◽  
Response Curves ◽  
Linear Fitting ◽  
Mlr Model

Cell viability evaluation is significantly meaningful for cellular assays. Some cells with weak viability are easily killed in the detection of anti-cancer drugs, while others with strong viability survive and proliferate, ultimately leading to the treatment failure or the inaccuracy of biological assays. Accurately evaluating cell viability heterogeneity still remains difficult. This paper proposed a multi-physical property information fusion method for evaluating cell viability heterogeneity based on multiple linear regression (MLR) on a single-channel integrated microfluidic chip. In this method, adhesion strengths τN, that are defined as the magnitude of shear stress needed to detach (100-N) % of cell population, were extracted as the independent variables of MLR model by calculating the linear fitting of the impedance-response curves for shear stress (cell detachment assay). Besides, by calculating the non-linear fitting of the drug dose-response curves for cancer cells (IC50 assay), the half-maximal inhibitory concentration (IC50) was extracted as the dependent variables of MLR model. The results show that the mean relative error of our fusion method reduces by 17.87% and 59.66% compared with the single-parameter method and the cell counting method. Moreover, through the theoretical analysis of the drug resistance heterogeneity model, it proved that there is a qualitative relationship between the cell adhesion strength and cell viability heterogeneity, which provides a theoretical basis for our fusion method.

Sign in / Sign up

Export Citation Format

Share Document