Diverse neurotoxicants converge on gene expression for neuropeptides and their receptors in an in vitro model of neurodifferentiation: Effects of chlorpyrifos, diazinon, dieldrin and divalent nickel in PC12 cells

Regulation of iron absorption by duodenal enterocytes is essential for the maintenance of homeostasis by preventing iron deficiency or overload. Despite the identification of a number of genes implicated in iron absorption and its regulation, it is likely that further factors remain to be identified. For that purpose, we used a global transcriptomic approach, using the CaCo-2 cell line as an in vitro model of intestinal absorptive cells. Pangenomic screening for variations in gene expression correlating with intracellular iron content allowed us to identify 171 genes. One hundred nine of these genes are clustered into five types of expression profile. This is the first time that most of these genes have been associated with iron metabolism. Functional annotation of these five clusters indicates potential links between the immune response, proteolysis processes, and iron depletion. In contrast, iron overload is associated with cellular metabolism, especially that of lipids and glutathione involving redox function and electron transfer.

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In vitro model of angiogenesis using a human endothelium-derived permanent cell line: Contributions of induced gene expression, G-proteins, and integrins

Journal of Cellular Physiology ◽

10.1002/jcp.1041530302 ◽

1992 ◽

Vol 153 (3) ◽

pp. 437-449 ◽

Cited By ~ 136

Author(s):

Jeffrey Bauer ◽

Michael Margolis ◽

Clara Schreiner ◽

Cora-Jean Edgell ◽

Jane Azizkhan ◽

...

Keyword(s):

Gene Expression ◽

Cell Line ◽

G Proteins ◽

In Vitro Model ◽

Permanent Cell Line ◽

Permanent Cell ◽

Vitro Model ◽

Human Endothelium

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Establishment and characterization of a piscean PCF cell line for toxicity and gene expression studies as in vitro model

Tissue and Cell ◽

10.1016/j.tice.2014.04.004 ◽

2014 ◽

Vol 46 (3) ◽

pp. 206-212 ◽

Cited By ~ 6

Author(s):

M. Goswami ◽

B.S. Sharma ◽

Kamalendra Yadav ◽

S.N. Bahuguna ◽

W.S. Lakra

Keyword(s):

Gene Expression ◽

Cell Line ◽

In Vitro Model ◽

Expression Studies ◽

Gene Expression Studies ◽

Vitro Model

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Gene expression changes in an in vitro model of chondroinduction: a comparison of two methods

Wound Repair and Regeneration ◽

10.1046/j.1524-475x.2003.11512.x ◽

2003 ◽

Vol 11 (5) ◽

pp. 386-392 ◽

Cited By ~ 4

Author(s):

Karen E. Yates ◽

Julie Glowacki

Keyword(s):

Gene Expression ◽

In Vitro Model ◽

Vitro Model

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Characterisation of an in vitro system to study maternal communication with spermatozoa

Reproduction Fertility and Development ◽

10.1071/rd11268 ◽

2012 ◽

Vol 24 (7) ◽

pp. 988 ◽

Cited By ~ 7

Author(s):

Ahmed Aldarmahi ◽

Sarah Elliott ◽

Jean Russell ◽

Thomas Klonisch ◽

Sabine Hombach-Klonisch ◽

...

Keyword(s):

Gene Expression ◽

Epithelial Cells ◽

In Vitro Model ◽

Epithelial Cell Line ◽

Prostaglandin E ◽

Sperm Viability ◽

Cell Passage ◽

In Vitro System ◽

Vitro Model

In vivo, gamete maturation, fertilisation and early embryonic development take place inside the oviduct. Several studies have indicated that local responses towards gametes and embryos are generated by the maternal reproductive tract. However, no defined in vitro model currently exists to allow detailed and systematic investigation of maternal communications with gametes and embryos. Therefore, we characterised an in vitro model based on the interaction of boar spermatozoa with an immortalised porcine oviduct epithelial cell line to evaluate different factors that may affect this model. The factors tested were sperm viability, source of spermatozoa, cell passage effect and the effect of reproductive and non-reproductive epithelial cells in the interaction with spermatozoa. After 24 h of co-incubation, RNA was extracted and used to synthesise cDNA for quantitative real-time PCR. Alteration in the expression of genes such as adrenomedullin, heat-shock 70-kDa protein 8 and prostaglandin E synthase was considered as the end point of this assay. The results showed that sperm viability and cell passage number had an effect on oviductal gene expression in response to spermatozoa. Oviductal cells showed significant alterations in gene expression when compared with non-reproductive epithelial cells. The simple in vitro system described here has potential application for further studies in our understanding of mechanisms involved in maternal interactions with spermatozoa.

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The transcriptional landscape of the murine middle ear epithelium in vitro

10.1101/800987 ◽

2019 ◽

Author(s):

Apoorva Mulay ◽

Md Miraj K Chowdhury ◽

Cameron James ◽

Lynne Bingle ◽

Colin D Bingle

Keyword(s):

Gene Expression ◽

Middle Ear ◽

In Vitro Model ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Inflammatory Cells ◽

Differentially Expressed ◽

Vitro Model ◽

Transcriptional Landscape

AbstractOtitis media (OM) is the most common paediatric disease and leads to significant morbidity. Although understanding of underlying disease mechanisms is hampered by complex pathophysiology, it is clear that epithelial abnormalities underpin the disease. The mechanisms underpinning epithelial remodelling in OM remain unclear. We recently described a novel in vitro model of mouse middle ear epithelial cells (mMEECs) that undergoes mucociliary differentiation into the varied epithelial cell populations seen in the middle ear cavity. We now describe genome wide gene expression profiles of mMEECs as they undergo differentiation. We compared the gene expression profiles of original (uncultured) middle ear cells, confluent cultures of undifferentiated cells (day 0 of ALI) and cells that had been differentiated for 7 days at an ALI. >5000 genes were differentially expressed among the three groups of cells. Approximately 4000 genes were differentially expressed between the original cells and day 0 of ALI culture. The original cell population was shown to contain a mix of cell types, including contaminating inflammatory cells that were lost on culture. Approximately 500 genes were upregulated during ALI induced differentiation. These included some secretory genes and some enzymes but most were associated with the process of ciliogenesis. Our in vitro model of differentiated murine middle ear epithelium exhibits a transcriptional profile consistent with the mucociliary epithelium seen within the middle ear. Knowledge of the transcriptional landscape of this epithelium will provide a basis for understanding the phenotypic changes seen in murine models of OM.

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