Evolution of cerebral perfusion in the peri-contusional cortex in mice revealed by in vivo laser speckle imaging after traumatic brain injury

Speckle simulation is a powerful protocol to investigate the properties of speckle and evaluate image processing method. However, only static speckle images can be simulated by available methods without considering time-integrated effect of CCD. A time–integrated dynamic speckle simulation method basedon coherent imaging was developed. Through the new simulation method, the effect of speckle size on LSCI was investigated. The smaller the speckle size is, the higher the spatial resolution become.But the one-dimensional speckle size should exceed two pixels to sample the speckle pattern. The characteristics of existing speckle contrast imaging methods were studied based on spatial statistics, and optimal parameters are given to obtain accurate and less noisy image. In general, the new simulation method for laser speckle imaging is a powerful tool to monitor blood flow in vivo and lay a solid foundation for the study of hemodynamics.

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Comparison of in-vivo optical imaging of brain functional activity: intrinsic signal imaging and laser speckle imaging

10.1117/12.462531 ◽

2002 ◽

Author(s):

Zheng Wang ◽

Qingming Luo ◽

Haiying Cheng

Keyword(s):

Optical Imaging ◽

Functional Activity ◽

Laser Speckle ◽

Laser Speckle Imaging ◽

Speckle Imaging ◽

Intrinsic Signal ◽

In Vivo Optical Imaging

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High-speed dynamic laser speckle imaging of changes of microcirculation in vivo

10.1117/12.875699 ◽

2011 ◽

Author(s):

Jia Qin ◽

Lin An ◽

Ruikang Wang

Keyword(s):

High Speed ◽

Laser Speckle ◽

Laser Speckle Imaging ◽

Speckle Imaging

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Theranostic cranial implant for hyperspectral light delivery and microcirculation imaging without scalp removal

10.1101/720599 ◽

2019 ◽

Author(s):

Nami Davoodzadeh ◽

Mildred S. Cano-Velázquez ◽

Carrie R. Jonak ◽

David L. Halaney ◽

Devin K. Binder ◽

...

Keyword(s):

Signal To Noise Ratio ◽

Image Resolution ◽

Laser Speckle ◽

Laser Speckle Imaging ◽

Speckle Imaging ◽

Optical Access ◽

Optical Attenuation ◽

Cranial Implant ◽

The Brain

Light based techniques for imaging, diagnosing and treating the brain have become widespread clinical tools, but application of these techniques is limited by optical attenuation in the scalp and skull. This optical attenuation reduces the achievable spatial resolution, precluding the visualization of small features such as brain microvessels. The goal of this study was to assess a strategy for providing ongoing optical access to the brain without the need for repeated craniectomy or retraction of the scalp. This strategy involves the use of a transparent cranial implant and skin optical clearing agents, and was tested in mice to assess improvements in optical access which could be achieved for laser speckle imaging of cerebral microvasculature. Combined transmittance of the optically cleared scalp overlying the transparent cranial implant was as high as 89% in the NIR range, 50% in red range, 24% in green range, and 20% in blue range. In vivo laser speckle imaging experiments of mouse cerebral blood vessels showed that the proposed optical access increased signal-to-noise ratio and image resolution, allowing for visualization of microvessels through the transparent implant, which was not possible through the uncleared scalp and intact skull.

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