scholarly journals SSR markers associated to early leaf spot disease resistance through selective genotyping and single marker analysis in groundnut ( Arachis hypogaea L.)

2017 ◽  
Vol 15 ◽  
pp. 132-137 ◽  
Author(s):  
Adama Zongo ◽  
Pawan Khera ◽  
Mahamadou Sawadogo ◽  
Yaduru Shasidhar ◽  
Manda Sriswathi ◽  
...  
2016 ◽  
Vol 16 (3) ◽  
pp. 189-196 ◽  
Author(s):  
Gustavo H.F. Klabunde ◽  
Camila F.O. Junkes ◽  
Sarah Z.A. Tenfen ◽  
Adriana C.M. Dantas ◽  
Carla R.C. Furlan ◽  
...  

1999 ◽  
Vol 26 (1) ◽  
pp. 4-8 ◽  
Author(s):  
J. A. Baysinger ◽  
H. A. Melouk ◽  
D. S. Murray

Abstract Early leaf spot is a common disease of peanut caused by the fungus Cercospora arachidicola Hori. Experiments were conducted to evaluate the effect of postemergence herbicides on the conidial germination of C. arachidicola and on the incidence of early leaf spot disease in peanut (Arachis hypogaea L.) in a greenhouse. Conidial germination was enhanced (≥ 100%) at concentrations of 1, 100, and 1000 mg/L of 2,4-DB compared with the untreated control. Lactofen reduced conidial germination by 42% compared with the control at concentrations as low as 100 mg/L and completely inhibited germination at concentrations ≥ 5000 mg/L. A concentration of 10,000 mg/L acifluorfen and 2,4-DB completely inhibited conidial germination. Acifluorfen, acifluorfen plus 2,4-DB, and lactofen decreased the sporulation of early leaf spot lesions. Lactofen reduced leaf spot incidence 12% and decreased sporulation of lesions 22% compared with the control. None of the herbicides increased the incidence of early leaf spot on peanut plants or the number of early leaf spot lesions per leaflet when compared with plants that received no herbicide.


1998 ◽  
Vol 25 (1) ◽  
pp. 35-39 ◽  
Author(s):  
W. J. Grichar ◽  
B. A. Besler ◽  
A. J. Jaks

Abstract Peanut (Arachis hypogaea L.) pod yield and response to early and late leaf spots [caused by Cercospora arachidicola S. Hori and Cercosporidium personatum (Berk. & M. A. Curtis) Deighton, respectively] were evaluated on six runner-type cultivars under four leaf spot spray programs using tebuconazole at 0.23 kg ai/ha and chlorothalonil at 1.26 kg ai/ha. The four leaf spot spray programs included unsprayed, 14-d schedule, 21-d schedule, and 28-d schedule. With the 14- and 21-d schedule, chlorothalonil was applied at the first and last applications with a maximum of four tebuconazole applications for the middle sprays. On the 28-d schedule, tebuconazole was applied four times. Under conditions of heavy leaf spot disease pressure where no fungicide was applied, Southern Runner and Georgia Browne were slightly less susceptible (although not significantly) to early or late leaf spot than Florunner, GK-7, Georgia Runner, or Sunrunner. Less leaf spot was present in the 14-d schedule compared to 21- or 28-d schedules. Although there was no yield difference between the 14-, 21-, or 28-d schedules, the plots sprayed on a 14-d schedule yielded 43% more than the unsprayed. When averaged across all spray schedules, Georgia Browne yielded 15% more peanuts than Georgia Runner.


2005 ◽  
Vol 95 (4) ◽  
pp. 381-387 ◽  
Author(s):  
M. Luo ◽  
P. Dang ◽  
M. G. Bausher ◽  
C. C. Holbrook ◽  
R. D. Lee ◽  
...  

Late leaf spot disease caused by Cercosporidium personatum is one of the most destructive foliar diseases of peanut (Arachis hypogaea) worldwide. The objective of this research was to identify resistance genes in response to leaf spot disease using microarray and real-time polymerase chain reaction (PCR). To identify transcripts involved in disease resistance, we studied the gene expression profiles in two peanut genotypes, resistant or susceptible to leaf spot disease, using cDNA microarray containing 384 unigenes selected from two expressed sequenced tag (EST) cDNA libraries challenged by abiotic and biotic stresses. A total of 112 spots representing 56 genes in several functional categories were detected as up-regulated genes (log2 ratio > 1). Seventeen of the top 20 genes, each matching gene with known function in GenBank, were selected for validation of their expression levels using real-time PCR. The two peanut genotypes were also used to study the functional analysis of these genes and the possible link of these genes to the disease resistance trait. Microarray technology and real-time PCR were used for comparison of gene expression. The selected genes identified by microarray analysis were validated by real-time PCR. These genes were more greatly expressed in the resistant genotype as a result of response to the challenge of C. personatum than in the susceptible genotype. Further investigations are needed to characterize each of these genes in disease resistance. Gene probes could then be developed for application in breeding programs for marker-assisted selection.


Author(s):  
S. R. Zanjare ◽  
A. V. Suryawanshi ◽  
Snehal S. Zanjare ◽  
V. R. Shelar ◽  
Y. S. Balgude

Background: Groundnut (Arachis hypogaea L.) is the fourth most important oil seed crop in the world. The early and late spots are a foliage disease more common and more destructive of groundnut which cause severe yield losses up to 70% and reduces the quality of the pod and fodder. Recent efforts have targeted on developing resistant source against leaf spot disease which are helpful for discarding the hazardous toxic chemical compounds used for controlling the disease. The current study aimed to identify the sources of resistance to leaf spot from the newly identified genotypes as well as from wild species and to study the way in which these were responding to the to the disease development. Methods: Seventy three groundnut genotypes were screened against late leaf spot disease under artificial epiphytotic conditions in field at AICRP on Groundnut, MPKV, Rahuri during kharif 2015-16. The observations of disease intensity and rate of infection were recorded. Result: Among the Seventy three groundnut genotypes, 10 genotypes showed resistant and 33 genotypes showed the moderately resistant reaction to leaf spot disease. The 25 entries showed the susceptible and 05 genotypes showed the highly susceptible reaction to the leaf spot disease. All the susceptible cultivars belong to A. hypogaea sub. sp. fastigiata var. fastigiata to as Spanish bunch. These 10 resistant genotypes of groundnut including two wild species viz., RHRG 6083, KDG 128, GPBD 4, ICGV 94118, ICGV 4983, ICG 12672, ICGV 13160 (A. batizocoi), ICGV 13165 (A. cardenasi), ICG 11426 and ICGV8193 were selected for intensive artificial screening under glasshouse conditions and also confirmed resistance. The resistant lines will be useful to develop introgression lines using marker-assisted backcrossing approach to improve foliar fungal disease resistance in popular groundnut varieties.


2007 ◽  
Vol 24 (3) ◽  
pp. 138-143 ◽  
Author(s):  
J. Derera ◽  
P. Tongoona ◽  
B. S. Vivek ◽  
N. van Rij ◽  
M. D. Laing

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