Structural characteristics and anticancer activity in vitro of fucoidan from brown alga Padina boryana

The study presents the results of a comparative evaluation of the effect of structural modifications of fucoidans from the brown alga Fucus evanescens (native, highly purified product of fucoidan enzymatic hydrolysis, a new regular 1→3;1→4-α-L-fucan, sulphated mainly at C2 and acetylated at C4 of the fucose residue) on the effector functions of innate and adaptive immunity cells in vitro and in vivo. Using flow cytometry, we found that all examined fucoidans induce the maturation of dendritic cells, enhance the ability of neutrophils to migrate and adhere, activate monocytes and enhance their antigen-presenting functions, and increase the cytotoxic potential of natural killers. Fucoidans increase the production of hepatitis B virus (HBs) specific IgG and cytokine Th1 (IFN-γ, TNF-α) and Th2 (IL-4) profiles in vivo. The data obtained suggest that in vitro and in vivo adjuvant effects of the products of fucoidan enzymatic hydrolysis with regular structural characteristics are comparable to those of the native fucoidan. Based on these data, the products of fucoidan enzymatic hydrolysis can be considered as an effective and safe candidate adjuvant to improve the efficacy of prophylactic and therapeutic vaccines.

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Anticancer activity in vitro of a fucoidan from the brown alga Fucus evanescens and its low-molecular fragments, structurally characterized by tandem mass-spectrometry

Carbohydrate Polymers ◽

10.1016/j.carbpol.2011.07.036 ◽

2012 ◽

Vol 87 (1) ◽

pp. 186-194 ◽

Cited By ~ 71

Author(s):

Stanislav D. Anastyuk ◽

Natalia M. Shevchenko ◽

Svetlana P. Ermakova ◽

Olesya S. Vishchuk ◽

Evgeny L. Nazarenko ◽

...

Keyword(s):

Mass Spectrometry ◽

Tandem Mass Spectrometry ◽

Anticancer Activity ◽

Brown Alga ◽

Tandem Mass ◽

Molecular Fragments ◽

Fucus Evanescens

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Structural elucidation of polysaccharide fractions from the brown alga Coccophora langsdorfii and in vitro investigation of their anticancer activity

Carbohydrate Polymers ◽

10.1016/j.carbpol.2015.08.062 ◽

2016 ◽

Vol 135 ◽

pp. 162-168 ◽

Cited By ~ 34

Author(s):

Tatiana I. Imbs ◽

Svetlana P. Ermakova ◽

Olesya S. Malyarenko (Vishchuk) ◽

Vladimir V. Isakov ◽

Tatiana N. Zvyagintseva

Keyword(s):

Anticancer Activity ◽

Brown Alga ◽

Structural Elucidation ◽

In Vitro Investigation

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Isolation, characterization, in vitro anticancer activity, dft calculations, molecular docking, bioactivity score, drug-likeness and admet studies of eight phytoconstituents from brown alga sargassum platycarpum

Journal of Molecular Structure ◽

10.1016/j.molstruc.2020.129245 ◽

2021 ◽

Vol 1225 ◽

pp. 129245

Author(s):

Doaa A. Abdelrheem ◽

Aziz A. Rahman ◽

Khaled N.M. Elsayed ◽

H.R. Abd El-Mageed ◽

Hussein S. Mohamed ◽

...

Keyword(s):

Molecular Docking ◽

Dft Calculations ◽

Anticancer Activity ◽

Brown Alga

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Structural Characteristics and Anticancer Activity of Fucoidan from the Brown Alga Sargassum mcclurei

Marine Drugs ◽

10.3390/md11051456 ◽

2013 ◽

Vol 11 (5) ◽

pp. 1456-1476 ◽

Cited By ~ 59

Author(s):

Pham Thinh ◽

Roza Menshova ◽

Svetlana Ermakova ◽

Stanislav Anastyuk ◽

Bui Ly ◽

...

Keyword(s):

Anticancer Activity ◽

Brown Alga ◽

Structural Characteristics

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Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro

PeerJ ◽

10.7717/peerj.6409 ◽

2019 ◽

Vol 7 ◽

pp. e6409 ◽

Cited By ~ 5

Author(s):

Shengfeng Yang ◽

Haitao Wan ◽

Rui Wang ◽

Daijun Hao

Keyword(s):

Anticancer Activity ◽

Hepg2 Cells ◽

Phaeodactylum Tricornutum ◽

Structural Characteristics ◽

Sulfated Polysaccharide ◽

Biologically Active ◽

Sulfated Polysaccharides ◽

Dependent Manner ◽

Active Components

Microalgae, eukaryotic unicellular plants, are increasing in demand due to their use as nutraceutical and food supplements. They consisted different kinds of biologically active components such as polysaccharides. On the other hand, cancer is the leading cause of death globally. At present, there is no efficient method to cure it. Therefore, in this work, we extracted polysaccharides from Phaeodactylum tricornutum (PTP), characterized the chemical composition and structure, and investigated its anticancer activity on HepG2 cells. The results showed that PTP was a sulfated polysaccharide with a high Mw of 4,810 kDa, and xylose, fucose, glucose and galactose were the main monosaccharides. PTP has significant anticancer activity in a dose-dependent manner (up to 60.37% at 250 ug/mL) according to MTT assays. Furthermore, cycle analysis was carried out to explain its anticancer activity. The results showed that it exhibited anticancer effect mainly through the induction of apoptosis without affecting the cycle and mitosis of HepG2 cells. This might make it a potential drug for anticancer treatment in the future.

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Structure and anticancer activity in vitro of sulfated galactofucan from brown alga Alaria angusta

Carbohydrate Polymers ◽

10.1016/j.carbpol.2015.06.020 ◽

2015 ◽

Vol 132 ◽

pp. 118-125 ◽

Cited By ~ 37

Author(s):

Roza V. Menshova ◽

Stanislav D. Anastyuk ◽

Svetlana P. Ermakova ◽

Natalia M. Shevchenko ◽

Vladimir I. Isakov ◽

...

Keyword(s):

Anticancer Activity ◽

Brown Alga

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Use of light microscopy in the qualitative and quantitative study of liquid crystalline order

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100121727 ◽

1992 ◽

Vol 50 (1) ◽

pp. 264-265

Author(s):

Christopher Viney

Keyword(s):

Light Microscopy ◽

Liquid Crystalline ◽

Structural Characteristics ◽

Molecular Order ◽

Liquid Crystalline Phases ◽

Convenient Technique ◽

Liquid Crystalline Materials ◽

Transparent Windows

Light microscopy is a convenient technique for characterizing molecular order in fluid liquid crystalline materials. Microstructures can usually be observed under the actual conditions that promote the formation of liquid crystalline phases, whether or not a solvent is required, and at temperatures that can range from the boiling point of nitrogen to 600°C. It is relatively easy to produce specimens that are sufficiently thin and flat, simply by confining a droplet between glass cover slides. Specimens do not need to be conducting, and they do not have to be maintained in a vacuum. Drybox or other controlled environmental conditions can be maintained in a sealed chamber equipped with transparent windows; some heating/ freezing stages can be used for this purpose. It is relatively easy to construct a modified stage so that the generation and relaxation of global molecular order can be observed while specimens are being sheared, simulating flow conditions that exist during processing. Also, light only rarely affects the chemical composition or molecular weight distribution of the sample. Because little or no processing is required after collecting the sample, one can be confident that biologically derived materials will reveal many of their in vivo structural characteristics, even though microscopy is performed in vitro.

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