scholarly journals Optimization and characterization of bacterial nanocellulose produced by Komagataeibacter rhaeticus K3

2021 ◽  
Vol 2 ◽  
pp. 100022
Author(s):  
Paulina Jacek ◽  
Francisco A.G. Soares da Silva ◽  
Fernando Dourado ◽  
Stanisław Bielecki ◽  
Miguel Gama
2020 ◽  
Vol 69 (5-6) ◽  
pp. 295-302
Author(s):  
Nikolina Mrkonjić ◽  
Tamara Holjevac Grgurić ◽  
Vedrana Špada ◽  
Igor Slivac ◽  
Emi Govorčin Bajsić ◽  
...  

Polilaktidna kiselina (PLA) i bakterijska nanoceluloza (BNC) zbog svoje biorazgradljivosti, biokompatibilnosti i netoksičnosti imaju velik potencijal za primjenu u biomedicini. Cilj ovog rada bio je pripraviti i ispitati biokompozit PLA/BNC. Istražen je utjecaj BNC-a na morfološku strukturu, kemijski sastav, toplinska svojstva, toplinsku postojanost i hidrofobnost PLA te zasijavanje i rast stanica biokompozita PLA/BNC primjenom pretražnog elektronskog mikroskopa (SEM), infracrvene spektroskopije (FTIR), diferencijalne pretražne kalorimetrije (DSC) i termogravimetrijske analize (TGA) te određivanjem kontaktnog kuta i metodom MTT. Dodatkom BNC-a u PLA dolazi do pomaka staklišta (<i>T</i><sub>g</sub>) prema nižim temperaturama, što ukazuje na veću pokretljivost amorfne faze PLA te porasta stupnja kristalnosti zbog nukleacijskog učinka celuloze. Početak toplinske razgradnje pomaknut je na niže temperature u odnosu na čisti PLA, što ukazuje na smanjenje toplinske postojanosti PLA dodatkom BNC-a. Biokompozit PLA/BNC pokazuje poroznu, vlaknastu strukturu. Test zasijavanja stanica pokazao je da je biokompozit PLA/BNC pogodan za prihvaćanje i rast humanih stanica, pa je prema tome potencijalno primjenjiv u regenerativnoj medicini i tkivnom inženjerstvu.


2019 ◽  
Vol 34 (3) ◽  
pp. 339-350 ◽  
Author(s):  
Rose Ann Gabriel Franco ◽  
Andrew R Padalhin ◽  
John Patrick Cuenca ◽  
Reiza Ventura ◽  
Andrew Montecillo ◽  
...  

2017 ◽  
Vol 9 (22) ◽  
pp. 19048-19056 ◽  
Author(s):  
Rahul Mangayil ◽  
Satu Rajala ◽  
Arno Pammo ◽  
Essi Sarlin ◽  
Jin Luo ◽  
...  

Author(s):  
Bili Darnanto Susilo ◽  
Heru Suryanto ◽  
Aminnudin Aminnudin

Bacterial cellulose (BC) was synthesized from pineapple peel extract media with addition of fermentation agent bacteria Acetobacter xylinum. BC was disintegrated from the pellicle into bacterial nanocellulose (BNC) by using a high-pressure homogenizer (hph) machine, which has a three-dimensional woven nanofibrous network. The synthesis of composite films started when BNC, graphite nanoplatelets, and cetyltrimethylammonium bromide (CTAB) were homogenized using an ultrasonic homogenizer then baked on a glass mold at a temperature of 80 degrees Celcius for 14h. A scanning electron microscope (SEM) was used to analyze its morphology. X-Ray diffraction spectra were used to analyze the composite films structure. The functional groups of the composite films were analyzed using the FTIR spectrum. SEM micrograph shows that GNP was evenly distributed into BNC matrix after CTAB addition. GNPs are shown as flat and smooth flakes with sharp corners. Some peak corresponds O-H, C-H, C≡C, and CH3 stretching was identified by using FTIR spectroscopy at wavenumber 3379, 2893, 2135, and 1340 cm-1, respectively. XRD analysis shows that Crystalline Index (C.I) of BNC increases after 2.5 wt% addition of GNP. The presence of CTAB decreases C.I value of composite films. BNC/GNP composite films have the best mechanical properties with Young’s modulus about 77.01 ± 8.564.


Author(s):  
B. L. Soloff ◽  
T. A. Rado

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.


Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.


Author(s):  
B. H. Kear ◽  
J. M. Oblak

A nickel-base superalloy is essentially a Ni/Cr solid solution hardened by additions of Al (Ti, Nb, etc.) to precipitate a coherent, ordered phase. In most commercial alloy systems, e.g. B-1900, IN-100 and Mar-M200, the stable precipitate is Ni3 (Al,Ti) γ′, with an LI2structure. In A lloy 901 the normal precipitate is metastable Nis Ti3 γ′ ; the stable phase is a hexagonal Do2 4 structure. In Alloy 718 the strengthening precipitate is metastable γ″, which has a body-centered tetragonal D022 structure.Precipitate MorphologyIn most systems the ordered γ′ phase forms by a continuous precipitation re-action, which gives rise to a uniform intragranular dispersion of precipitate particles. For zero γ/γ′ misfit, the γ′ precipitates assume a spheroidal.


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