Structural analysis of the oligosaccharide of Histophilus somni (Haemophilus somnus) strain 2336 and identification of several lipooligosaccharide biosynthesis gene homologues

2005 ◽  
Vol 340 (4) ◽  
pp. 665-672 ◽  
Author(s):  
Frank St. Michael ◽  
Jianjun Li ◽  
Michael D. Howard ◽  
A. Jane Duncan ◽  
Thomas J. Inzana ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Histophilus Somni ◽  
Biosynthesis Gene ◽  
Haemophilus Somnus

Structural analysis of the lipooligosaccharide from the commensal Haemophilus somnus genome strain 129Pt

2004 ◽  
Vol 339 (3) ◽  
pp. 529-535 ◽  
Author(s):  
Frank St. Michael ◽  
Michael D. Howard ◽  
Jianjun Li ◽  
A. Jane Duncan ◽  
Thomas J. Inzana ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Haemophilus Somnus

scholarly journals Detecção de Histophilus somni (Haemophilus somnus) no sêmen bovino mediante reação em cadeia pela polimerase (PCR)

2014 ◽  
Vol 12 (1) ◽  
pp. 11
Author(s):  
Francisca Elda Ferreira Dias ◽  
Cáris Maroni Nunes ◽  
Tânia Vasconcelos Cavalcante ◽  
Juliano Franco De Souza ◽  
Silvia Minharro Barbosa ◽  
...  
Keyword(s):  
Histophilus Somni ◽  
Haemophilus Somnus

O presente estudo avaliou o uso da PCR para a detecção do Histophilus somni no sêmen bovino. Amostras de sêmen foram contaminadas experimentalmente com H. somni diluída em escalas de 107 a 101 bactérias/mL, submetidas à extração de DNA pelo método de fenol/clorofórmio e amplificadas pela PCR. Os produtos da amplificação do DNA foram analisados por eletroforese em gel de acrilamida 8%. Por meio de oligonucleotídeos iniciadores obteve-se a amplificação de um fragmento de 400 pares de bases a partir do DNA da bactéria. Conseguiu-se amplificação positiva até na diluição de 101 bactérias/mL. A PCR mostrou-se eficiente na detecção de H. somni. O resultado disponibiliza conhecimento relevante para o diagnóstico de H. somni, justificando a necessidade do diagnóstico dessa bactéria em reprodutores, especialmente em amostras de sêmen, que deveriam estar livres de qualquer contaminação. A PCR mostrou-se como uma valiosa ferramenta no controle da qualidade do sêmen produzido em centrais de inseminação artificial.

scholarly journals Mutation of an Arginine Biosynthesis Gene Causes Reduced Pathogenicity in Fusarium oxysporum f. sp. melonis

2001 ◽  
Vol 14 (4) ◽  
pp. 580-584 ◽  
Author(s):  
Fumio Namiki ◽  
Michiko Matsunaga ◽  
Mitsuru Okuda ◽  
Iori Inoue ◽  
Kazufumi Nishi ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Fusarium Oxysporum ◽  
Restriction Enzyme ◽  
Argininosuccinate Lyase ◽  
Arginine Biosynthesis ◽  
Biosynthesis Gene ◽  
Remi Mutagenesis ◽  
Auxotrophic Mutation ◽  
Arginine Auxotroph

Restriction enzyme-mediated integration (REMI) mutagenesis was used to tag genes required for pathogenicity of Fusarium oxysporum f. sp. melonis. Of the 1,129 REMI transformants tested, 13 showed reduced pathogenicity on susceptible melon cultivars. One of the mutants, FMMP95–1, was an arginine auxotroph. Structural analysis of the tagged site in FMMP95-1 identified a gene, designated ARG1, which possibly encodes argininosuccinate lyase, catalyzing the last step for arginine biosynthesis. Complementation of FMMP95–1 with the ARG1 gene caused a recovery in pathogenicity, indicating that arginine auxotrophic mutation causes reduced pathogenicity in this pathogen.

scholarly journals Structural analysis of the phase-variable lipooligosaccharide from Haemophilus somnus strain 738

1998 ◽  
Vol 253 (2) ◽  
pp. 507-516 ◽  
Author(s):  
Andrew D. Cox ◽  
Michael D. Howard ◽  
Jean-Robert Brisson ◽  
Mark van der Zwan ◽  
Pierre Thibault ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Phase Variable ◽  
Haemophilus Somnus

scholarly journals Targeted Disruption of a Melanin Biosynthesis Gene Affects Conidial Development and UV Tolerance in the Japanese Pear Pathotype of Alternaria alternata

1999 ◽  
Vol 12 (1) ◽  
pp. 59-63 ◽  
Author(s):  
Chie Kawamura ◽  
Takashi Tsujimoto ◽  
Takashi Tsuge
Keyword(s):  
Structural Analysis ◽  
Alternaria Alternata ◽  
Uv Light ◽  
Japanese Pear ◽  
Wild Type ◽  
Targeted Disruption ◽  
Melanin Biosynthesis ◽  
Uv Tolerance ◽  
Biosynthesis Gene ◽  
Conidial Development

Structural analysis of the BRM2 gene involved in melanin biosynthesis of the Japanese pear pathotype of Alternaria alternata suggested that this gene encodes 1,3,8-trihydroxynaphthalene reductase. Targeted disruption of the BRM2 gene did not affect pathogenicity, vegetative growth, or the number of conidia produced. Targeted disruption, however, did reduce conidial size and septal number, suggesting that melanin is associated with conidial development. The conidia of brm2 mutant transformants were more sensitive to UV light than those of the wild type, demonstrating that melanin confers UV tolerance.

scholarly journals A Case of Myocardial and Diaphragmatic Abscesses Caused by Histophilus somni (Haemophilus somnus) in Japanese Black Cattle

2005 ◽  
Vol 58 (4) ◽  
pp. 275-277
Author(s):  
Eiji YOKOYAMA ◽  
Satoshi MURAKAMI ◽  
Hiroyasu YAMADA ◽  
Junko KURODA ◽  
Kazunori KISIDA
Keyword(s):  
Japanese Black Cattle ◽  
Histophilus Somni ◽  
Haemophilus Somnus

scholarly journals Complete Genome Sequence of Haemophilus somnus (Histophilus somni) Strain 129Pt and Comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd

2006 ◽  
Vol 189 (5) ◽  
pp. 1890-1898 ◽  
Author(s):  
Jean F. Challacombe ◽  
A. J. Duncan ◽  
Thomas S. Brettin ◽  
David Bruce ◽  
Olga Chertkov ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Genome Sequence ◽  
Systemic Disease ◽  
Opportunistic Pathogen ◽  
Haemophilus Ducreyi ◽  
Histophilus Somni ◽  
Mucosal Surfaces ◽  
A Genome ◽  
Haemophilus Somnus ◽  
Protective Strategies

ABSTRACT Haemophilus somnus can be either a commensal of bovine mucosal surfaces or an opportunistic pathogen. Pathogenic strains of H. somnus are a significant cause of systemic disease in cattle. We report the genome sequence of H. somnus 129Pt, a nonpathogenic commensal preputial isolate, and the results of a genome-wide comparative analysis of H. somnus 129Pt, Haemophilus influenzae Rd, and Haemophilus ducreyi 35000HP. We found unique genes in H. somnus 129Pt involved in lipooligosaccharide biosynthesis, carbohydrate uptake and metabolism, cation transport, amino acid metabolism, ubiquinone and menaquinone biosynthesis, cell surface adhesion, biosynthesis of cofactors, energy metabolism, and electron transport. There were also many genes in common among the three organisms. Our comparative analyses of H. somnus 129Pt, H. influenzae Rd, and H. ducreyi 35000HP revealed similarities and differences in the numbers and compositions of genes involved in metabolism, host colonization, and persistence. These results lay a foundation for research on the host specificities and niche preferences of these organisms. Future comparisons between H. somnus 129Pt and virulent strains will aid in the development of protective strategies and vaccines to protect cattle against H. somnus disease.

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