Effects of Andrographis paniculata extract and Andrographolide on hepatic cytochrome P450 mRNA expression and monooxygenase activities after in vivo administration to rats and in vitro in rat and human hepatocyte cultures

2009 ◽  
Vol 179 (2-3) ◽  
pp. 247-255 ◽  
Author(s):  
Dumrongsak Pekthong ◽  
Nadège Blanchard ◽  
Catherine Abadie ◽  
Alexandre Bonet ◽  
Bruno Heyd ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Mrna Expression ◽  
Human Hepatocyte ◽  
Andrographis Paniculata ◽  
Hepatocyte Cultures ◽  
In Vivo Administration ◽  
Hepatic Cytochrome

scholarly journals beta-Naphthoflavone abolishes interrenal sensitivity to ACTH stimulation in rainbow trout

1998 ◽  
Vol 157 (1) ◽  
pp. 63-70 ◽  
Author(s):  
JM Wilson ◽  
MM Vijayan ◽  
CJ Kennedy ◽  
GK Iwama ◽  
TW Moon
Keyword(s):  
Cytochrome P450 ◽  
Rainbow Trout ◽  
Acth Stimulation ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Treated Fish ◽  
First Time ◽  
Hepatic Cytochrome ◽  
Stimulation Of

We report for the first time that beta-naphthoflavone (BNF) abolishes ACTH stimulation of cortisol production in rainbow trout (Oncorhynchus mykiss). There was significantly higher hepatic cytochrome P450 content and ethoxyresorufin O-de-ethylase and uridine-5'-diphosphoglucuronic acid transferase activities in BNF-treated fish than in sham-treated controls. BNF did not significantly affect either plasma turnover or tissue distribution of [3H]cortisol-derived radioactivity. Hepatic membrane fluidity and hepatocyte capacity for cortisol uptake were not altered by BNF as compared with the sham-treated fish. These results taken together suggest that BNF does not affect cortisol-clearance mechanisms in trout. A 3 min handling disturbance period elicited a plasma cortisol response in the sham-treated fish; however, the response in the BNF-treated fish was muted and significantly lower than in the sham fish. This in vivo response corroborates the lack of interrenal sensitivity to ACTH in vitro in the BNF-treated fish, suggesting that BNF affects the ACTH pathway in trout. Our results suggest the possibility that cytochrome P450-inducing compounds may affect cortisol dynamics by decreasing interrenal responsiveness to ACTH stimulation in fish, thereby impairing the physiological responses that are necessary for the animal to cope with the stressor.

In Vitro and In Vivo Evaluation of the Effect of Puerarin on Hepatic Cytochrome P450-Mediated Drug Metabolism

Planta Medica ◽  
2014 ◽  
Vol 80 (07) ◽  
pp. 561-567 ◽  
Author(s):  
Sang-Bum Kim ◽  
In-Soo Yoon ◽  
Kyu-Sang Kim ◽  
Sung-Jun Cho ◽  
Yeong Kim ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Drug Metabolism ◽  
In Vivo Evaluation ◽  
Hepatic Cytochrome

The Antiaggregating Activity of Clopidogrel Is due to a Metabolic Activation by the Hepatic Cytochrome P450-1A

1994 ◽  
Vol 72 (02) ◽  
pp. 313-317 ◽  
Author(s):  
P Savi ◽  
J Combalbert ◽  
C Gaich ◽  
M-C Rouchon ◽  
J-P Maffrand ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Metabolic Activation ◽  
Cytochrome P450 Enzymes ◽  
Specific Antibodies ◽  
Specific Induction ◽  
Antithrombotic Effects ◽  
Cytochrome P450 1A ◽  
Hepatic Cytochrome

SummaryClopidogrel and ticlopidine are two well known selective anti-ADP agents which are inactive in vitro and must be administered in vivo to fully exhibit their antiaggregating and antithrombotic effects. Since previous studies have clearly demonstrated that the activation steps take place in the liver, we examined the effect of specific induction or inhibition of cytochrome P450 subfamilies on the antiaggregating activity of clopidogrel. SKF 525-A, a global cytochrome P450 inhibitor, dramatically decreased the antiaggregating effect of clopidogrel, therefore indicating that cytochrome P450 enzymes are involved in the hepatic activation of clopidogrel. The efficacy of clopidogrel was increased in animals pretreated with 3-methylcholanthrene and (3-naphthoflavone, indicating that the cytochrome P450-1A subfamily pathway was mainly involved in the activating metabolism of clopidogrel. The use of specific antibodies directed against the various cytochrome P450 subfamilies ascertained this observation.

In vitro determination of the effect of Andrographis paniculata extracts and andrographolide on human hepatic cytochrome P450 activities

2011 ◽  
Vol 65 (3-4) ◽  
pp. 440-447 ◽  
Author(s):  
Yan Pan ◽  
Badrul Amini Abd-Rashid ◽  
Zakiah Ismail ◽  
Rusli Ismail ◽  
Joon Wah Mak ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Andrographis Paniculata ◽  
Hepatic Cytochrome
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