Effects of Andrographis paniculata crude extract and Andrographolide on hepatic cytochrome P450 mRNA expression and monooxygenase activities

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
D Pekthong ◽  
N Blanchard ◽  
C Abadie ◽  
A Bonet ◽  
B Heyd ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Mrna Expression ◽  
Crude Extract ◽  
Andrographis Paniculata ◽  
Hepatic Cytochrome

Impact of Andrographis paniculata crude extract on mouse hepatic cytochrome P450 enzymes

2006 ◽  
Vol 105 (3) ◽  
pp. 464-467 ◽  
Author(s):  
Kanokwan Jarukamjorn ◽  
Kannika Don-in ◽  
Cattaleya Makejaruskul ◽  
Thewarach Laha ◽  
Supawadee Daodee ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Crude Extract ◽  
Andrographis Paniculata ◽  
Cytochrome P450 Enzymes ◽  
Hepatic Cytochrome

Differential inhibition of rat and human hepatic cytochrome P450 by Andrographis paniculata extract and andrographolide

2008 ◽  
Vol 115 (3) ◽  
pp. 432-440 ◽  
Author(s):  
D. Pekthong ◽  
H. Martin ◽  
C. Abadie ◽  
A. Bonet ◽  
B. Heyd ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Andrographis Paniculata ◽  
Differential Inhibition ◽  
Hepatic Cytochrome

scholarly journals Time-Dependent Induction of Hepatic Cytochrome P450 Enzyme Activity and mRNA Expression by Bilobalide in Rats

2009 ◽  
Vol 109 (3) ◽  
pp. 459-462 ◽  
Author(s):  
Yuko Taki ◽  
Yuko Yamazaki ◽  
Fumio Shimura ◽  
Shizuo Yamada ◽  
Keizo Umegaki
Keyword(s):  
Enzyme Activity ◽  
Cytochrome P450 ◽  
Mrna Expression ◽  
Time Dependent ◽  
Cytochrome P450 Enzyme ◽  
P450 Enzyme ◽  
Hepatic Cytochrome

Salinomycin induces cell cycle arrest and apoptosis and modulates hepatic cytochrome P450 mRNA expression in HepG2/C3a cells

2021 ◽  
pp. 1-33
Author(s):  
Andressa Megumi Niwa ◽  
Simone Cristine Semprebon ◽  
Glaucia Fernanda Rocha D´Epiro ◽  
Lilian Areal Marques ◽  
Thalita Alves Zanetti ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cytochrome P450 ◽  
Mrna Expression ◽  
Cell Cycle Arrest ◽  
Cycle Arrest ◽  
Hepatic Cytochrome

In vitro determination of the effect of Andrographis paniculata extracts and andrographolide on human hepatic cytochrome P450 activities

2011 ◽  
Vol 65 (3-4) ◽  
pp. 440-447 ◽  
Author(s):  
Yan Pan ◽  
Badrul Amini Abd-Rashid ◽  
Zakiah Ismail ◽  
Rusli Ismail ◽  
Joon Wah Mak ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Andrographis Paniculata ◽  
Hepatic Cytochrome

scholarly journals Commiphora Myrrha (Nees) Engl. Resin Extracts Induce Phase-I Cytochrome P450 2C8, 2C9, 2C19, and 3A4 Isoenzyme Expressions in Human Hepatocellular Carcinoma (HepG2) Cells

2020 ◽  
Author(s):  
Zeyad Alehaideb ◽  
Ghada Alatar ◽  
Atef Nehdi ◽  
Abeer Albaz ◽  
Hamad Al-Eidi ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Protein Expression ◽  
Phase I ◽  
Mrna Expression ◽  
Crude Extract ◽  
Hepg2 Cells ◽  
Aqueous Extracts ◽  
Expression Levels ◽  
Gene And Protein Expression ◽  
Mrna Expression Levels

Abstract Background: Commiphora myrrha (Nees) Engl. (C. myrrha) resin is one of the oldest Middle Eastern herbal medicine used for treatment and prevention of numerous diseases. This resin is prepared in different methods and widely consumed among Saudi Arabian patients. Despite its popularity, no studies have been done on potential modulation effects of C. myrrha resin extracts on human cytochrome P450 (CYP) drug-metabolizing enzyme expression.Methods: The C. myrrha extracts were prepared by two different methods of sonication and boiling resembling the most popular traditional preparations of maceration and decoction, respectively. Both extracts were fingerprinted using high-performance liquid chromatography equipped with ultra-violet detector (HPLC-UVD). The viability of HepG2 cells treated with aqueous extracts was determined using Promega CellTiter-Glo® assay in order to select the efficient and non-toxic resin extract concentrations for phase-I metabolic isoenzyme expression analysis. The isoenzyme gene and protein expression levels of CYP 2C8, 2C9, 2C19, and 3A4 were measured using reverse transcription-quantitative polymerase chain reaction and Western blot technologies, respectively. Results: The HPLC-UVD fingerprinting revealed different chromatograms for C. myrrha extracts indicating possible differences in their modulation effects on CYP expression. Both aqueous extracts were toxic to HepG2 cells when tested at concentrations exceeding 150 µg/ml of dry crude extract. The CYP 2C8, 2C9, and 2C19 mRNA expression levels increased up to 4.0-fold after cell treatment with concentrations ranging from 1 to 30 µg/ml C. myrrha extracts, as compared with the untreated cells. However, the modulation of CYP3A4 mRNA expression levels was only significant at 30 µg/ml of crude extract exceeding the 2.0-fold cutoff. The up-regulation of CYP mRNA expression levels induced by C. myrrha extracts was confirmed at the CYP protein expression levels as well. Conclusions: The C. myrrha aqueous extracts modulate CYP 2C8, 2C9, 2C19, and 3A4 gene expression at clinically-relevant concentrations regardless of preparation methods. Further in vitro and in vivo experiments are required for the establishment of herb-drug interaction profile for these traditional medicinal resin extracts.

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