The entomotoxin Jack Bean Urease changes cathepsin D activity in nymphs of the hematophagous insect Dipetalogaster maxima (Hemiptera: Reduviidae)

2021 ◽  
Vol 251 ◽  
pp. 110511
Author(s):  
Natalia R. Moyetta ◽  
Leonardo L. Fruttero ◽  
Jimena Leyria ◽  
Fabian O. Ramos ◽  
Célia R. Carlini ◽  
...  
Keyword(s):  
Cathepsin D ◽  
Jack Bean Urease ◽  
Jack Bean ◽  
Cathepsin D Activity ◽  
Hematophagous Insect

scholarly journals Inhibition of jack bean urease by amphiphilic peptides

2021 ◽  
Author(s):  
Zafar Ali Shah ◽  
Sadam Hussain ◽  
Serab Khan ◽  
Nawab Ali ◽  
Samiullah Burki ◽  
...  
Keyword(s):  
Jack Bean Urease ◽  
Jack Bean ◽  
Amphiphilic Peptides

scholarly journals Oestradiol inhibits collagen breakdown in the involuting rat uterus

1972 ◽  
Vol 127 (4) ◽  
pp. 705-713 ◽  
Author(s):  
Janet N. Ryan ◽  
J. Frederick Woessner
Keyword(s):  
Cathepsin D ◽  
Density Gradient Centrifugation ◽  
Post Partum ◽  
Gradient Centrifugation ◽  
Specific Radioactivity ◽  
Rat Uterus ◽  
Oestradiol Treatment ◽  
Cathepsin D Activity ◽  
Stimulation Of

1. The earlier observation (Woessner, 1969) of oestradiol inhibition of collagen breakdown is confirmed and extended. Administration of 100μg of oestradiol-17β/day to parturient rats strongly inhibits the loss of collagen from the involuting uterus. Three experiments show that this effect is due to an inhibition of collagen degradation rather than to a stimulation of collagen synthesis. 2. Uterine collagen was labelled with hydroxy[14C]-proline by the administration of [14C]proline near the end of pregnancy. By 3 days post partum, control uteri lost 83% of their collagen and 90% of their hydroxy[14C]proline. Uteri from oestradiol-treated rats lost only 50% of both total and labelled hydroxyproline, with no decrease in the specific radioactivity of the hydroxyproline. 3. Incorporation of [14C]proline into uterine collagen hydroxyproline in vivo was not affected by oestradiol treatment. 4. Urinary excretion of hydroxyproline was increased in post-partum control rats and decreased in oestradiol-treated rats. 5. An enzyme capable of cleaving 4-phenylazobenzyloxycarbonyl-l-prolyl-l-leucylglycyl- l-prolyl-d-arginine (a substrate for clostridial collagenase) increased in activity in the post-partum uterus and was unaffected by oestradiol treatment. 6. Uterine homogenates digested uterine collagen extensively at pH3.2. This digestion was unaffected by the oestradiol treatment. 7. Lysosomal fractions prepared by density-gradient centrifugation of uterine homogenates contained coincident peaks of cathepsin D activity and peptide-bound hydroxyproline. The cathepsin D and hydroxyproline contents of this peak were unaffected by oestradiol treatment.

Single-step purification of urease by affinity chromatography

1974 ◽  
Vol 20 (4) ◽  
pp. 623-630 ◽  
Author(s):  
Bassanio L. Wong ◽  
Charles R. Shobe
Keyword(s):  
Enzyme Activity ◽  
Affinity Chromatography ◽  
Single Step ◽  
Jack Bean Urease ◽  
Jack Bean ◽  
Specific Activities ◽  
Single Step Purification

Single-step purification of urease (urea aminohydrolase; EC. 3.5.1.5) from cell-free extracts of Proteus morganii and from partially purified preparations of jack bean urease were achieved in less than 90 min by affinity chromatography on hydroxyurea-substituted beaded agarose columns. The specific activities of the purified enzymes were as high as, or higher than, those reported by other authors for urease preparations obtained by conventional techniques. In addition, yields of enzyme activity were routinely 6 to 100 times greater than recoveries previously reported for this enzyme.

A sensitive procedure for determination of cathepsin D: activity in alveolar and peritoneal macrophages

1984 ◽  
Vol 64 (2) ◽  
Author(s):  
Marian Orlowski ◽  
Robert Orlowski ◽  
JuiC. Chang ◽  
Elizabeth Wilk ◽  
Marvin Lesser
Keyword(s):  
Cathepsin D ◽  
Peritoneal Macrophages ◽  
Cathepsin D Activity

Cathepsin D Activity in Human Ovarian Tissues and Ovarian Carcinoma

1994 ◽  
Vol 1 (2) ◽  
pp. 173-177 ◽  
Author(s):  
Dalibor I. Hradek ◽  
Bruce Kessel ◽  
Nader Husseinzadeh ◽  
Bruce C. Moulton
Keyword(s):  
Ovarian Carcinoma ◽  
Cathepsin D ◽  
Cathepsin D Activity
Sign in / Sign up

Export Citation Format

Share Document