scholarly journals Evaluation of serum bone alkaline phosphatase activity in patients with liver disease: Comparison between electrophoresis and chemiluminescent enzyme immunoassay

2016 ◽  
Vol 460 ◽  
pp. 40-45 ◽  
Author(s):  
Fangjie Zhan ◽  
Yoshihisa Watanabe ◽  
Aya Shimoda ◽  
Etsuko Hamada ◽  
Yoshimasa Kobayashi ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Liver Disease ◽  
Enzyme Immunoassay ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Bone Alkaline Phosphatase ◽  
Serum Bone Alkaline Phosphatase ◽  
Chemiluminescent Enzyme Immunoassay

Quantitative fractionation of alkaline phosphatase isoenzymes according to their thermostability.

1976 ◽  
Vol 22 (1) ◽  
pp. 42-48 ◽  
Author(s):  
C PetitClerc
Keyword(s):  
Alkaline Phosphatase ◽  
Liver Disease ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Normal Serum ◽  
Transferase Activity ◽  
Gamma Glutamyl Transferase ◽  
Heat Labile ◽  
Alkaline Phosphatase Isoenzymes ◽  
Glutamyl Transferase

Abstract Continuous monitoring of heat denaturation of a mixture of alkaline phosphatase isoenzymes at 60 degrees C and pH 7.5 permits the simultaneous direct identification and quantitation of three isoenzymes: the placental isoenzyme, the L-phenylalanine-sensitive intestinal isoenzyme, and the liver isoenzyme (hepatocytic). The isoenzyme that is principally of bone origin cannot be identified as such without the help of other diagnostic aids and the patient's medical history. All human tissues contain alkaline phosphatase, many organs more than one of the isoenzymes. Liver alkaline phosphatase, which constitutes 40-50% of normal serum alkaline phosphatase activity, was measured in the serum of persons with various liver diseases. Its activity exceeded normal in all types of liver disease; in 80% of cases this increase was accompanied by increased gamma-glutamyl-transferase activity, but the quantitative correlationship (r = 0.54) was not as good as expected if both enzymes come from the same source and are indices of liver dieases. Liver alkaline phosphatase activity increases in the blood early in liver disease, before most liver tests show abnormalities. The other major isoenzyme of normal serum probably represents a mixture of isoenzymes from bone and reticulo-endothelial and vascular tissues, which all contain the same "very heat-labile" alkaline phosphatase. Cord blood and children's sera contain mostly this very heat-labile isoenzyme.

scholarly journals Evaluation of Serum Zinc Status and Serum Alkaline Phosphatase Activity in Alcoholic Liver Disease Patients - A Hospital Based Study from Chennai, Tamil Nadu

2021 ◽  
Vol 8 (24) ◽  
pp. 2100-2105
Author(s):  
Uma T ◽  
Nirmaladevi P ◽  
Shanthi R ◽  
Mahalakshmi R
Keyword(s):  
Alkaline Phosphatase ◽  
Liver Disease ◽  
Phosphatase Activity ◽  
Alcoholic Liver Disease ◽  
Alkaline Phosphatase Activity ◽  
Serum Zinc ◽  
Meld Score ◽  
Gamma Glutamyl Transferase ◽  
Zinc Status ◽  
Glutamyl Transferase

BACKGROUND Alcoholism remains to be the major cause of morbidity and mortality throughout the world. Consuming alcohol is the potent etiological factor for the development of alcoholic liver diseases (ALD), ranging from fatty liver to hepatocellular carcinoma with varying rates of development in both genders depending on the quality, quantity, and duration of the drink. Zinc deficiency has been documented with the progression of alcoholic liver disease. It is also a well-known fact that zinc is a co-factor for enzyme alkaline phosphatase. This study aims to assess the zinc status and alkaline phosphatase activity in patients with various stages of alcoholic liver disease, correlate zinc with alkaline phosphatase activity, albumin, gamma glutamyl transferase activity, MELD score and duration of alcohol intake and analysing the need for evaluating zinc in these patients. METHODS This comparative observational study involves group I healthy controls and group II patients diagnosed to have ethanol related decompensated liver disease with or without portal hypertension for more than three years from the Department of Medical Gastroenterology, Government Medical College Hospital. 5 ml of venous blood in fasting state was collected from both groups and assayed for serum zinc, and serum alkaline phosphatase activity. The data was statistically analysed. RESULTS The study results demonstrate that higher percentage of patients with alcoholic liver disease have low serum zinc levels than healthy controls. Zinc when compared with variables like serum albumin, duration of alcohol intake, MELD score, serum gamma glutamyl transferase and alkaline phosphatase in the case and control groups were found to be statistically significant. CONCLUSIONS There is decrease in serum zinc level and increased alkaline phosphatase activity in patients with alcoholic liver disease. The statistically significant data is a strong rationale for evaluating the zinc status and thereby supplementing zinc to patients with alcoholic liver disease. KEYWORDS Alcoholic Liver Disease, Zinc, Alkaline Phosphatase, MELD Score

The influence of ‘hypophysectomy’ on bone growth and alkaline phosphatase activity in the chick embryo

Development ◽  
1973 ◽  
Vol 29 (3) ◽  
pp. 515-527
Author(s):  
D. J. McWhinnie ◽  
Robert C. Thommes
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Bone Growth ◽  
Developmental Time ◽  
Bone Alkaline Phosphatase ◽  
Enzyme Synthesis ◽  
Skeletal Maturation ◽  
Specific Alkaline Phosphatase ◽  
Normal Increase

Wet weights, total bone alkaline phosphatase activity and specific alkaline phosphatase activity were determined on demarrowed femurs of normal, ‘hypophysectomized’ and pituitary-grafted chick embryos at selected intervals of incubation. In normal bones, all parameters noted above increased progressively through developmental time. ‘Hypophysectomy’ by means of surgical decapitation significantly retarded the normal increase of femur wet weight, total and specific alkaline phosphatase activity; in embryos bearing pituitary transplants, there was a return towards normal values. The possible role(s) of the pituitary in skeletal maturation and enzyme synthesis or activation is discussed.

Multicenter evaluation of Iso-ALP test kit for measurement of bone alkaline phosphatase activity in serum and plasma

1993 ◽  
Vol 39 (4) ◽  
pp. 648-652 ◽  
Author(s):  
S B Rosalki ◽  
A Y Foo ◽  
A Burlina ◽  
W Prellwitz ◽  
P Stieber ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Bone Alkaline Phosphatase ◽  
Alp Activity ◽  
Test Kit ◽  
Reference Limits ◽  
Bone Fraction ◽  
Adults And Children ◽  
Wheat Germ Lectin

Abstract A test kit (Iso-ALP, Boehringer Mannheim) for measuring human bone alkaline phosphatase activity in serum or plasma was evaluated in five laboratories in three countries. The assay is based on the principle described by Rosalki and Foo (Clin Chem 1984;30:1182-6) and uses wheat germ lectin to precipitate bone alkaline phosphatase. Residual ALP in the supernate in comparison with total ALP is used to quantify the bone fraction. The imprecision of residual ALP measurement was low (median between-run CV 4.9%) and comparable with that of total ALP. Linearity of precipitation was demonstrable up to a bone ALP activity (diethanolamine buffer 37 degrees C) of 2000 U/L, though a matrix effect was observed for dilutions of high-activity sera in saline or bovine serum albumin. For assaying patients' samples, different batches of lectin demonstrated excellent comparability. Taking electrophoresis as a basis for standardization, we determined that the lectin precipitated approximately 90% of bone ALP, but < 5% of nonbone ALP. From this we derived serum/plasma upper reference limits for bone ALP activity in adults and children.

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