The influence of ‘hypophysectomy’ on bone growth and alkaline phosphatase activity in the chick embryo

Development ◽  
1973 ◽  
Vol 29 (3) ◽  
pp. 515-527
Author(s):  
D. J. McWhinnie ◽  
Robert C. Thommes
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Bone Growth ◽  
Developmental Time ◽  
Bone Alkaline Phosphatase ◽  
Enzyme Synthesis ◽  
Skeletal Maturation ◽  
Specific Alkaline Phosphatase ◽  
Normal Increase

Wet weights, total bone alkaline phosphatase activity and specific alkaline phosphatase activity were determined on demarrowed femurs of normal, ‘hypophysectomized’ and pituitary-grafted chick embryos at selected intervals of incubation. In normal bones, all parameters noted above increased progressively through developmental time. ‘Hypophysectomy’ by means of surgical decapitation significantly retarded the normal increase of femur wet weight, total and specific alkaline phosphatase activity; in embryos bearing pituitary transplants, there was a return towards normal values. The possible role(s) of the pituitary in skeletal maturation and enzyme synthesis or activation is discussed.

Histochemical Distribution of 5-Nucleotidase in Snake Tissues: Presence of 5-Nucleotidase and Absence of Alkaline Phosphomonoesterase in Venom Glands of the Rattlesnake

1952 ◽  
Vol s3-93 (24) ◽  
pp. 391-394
Author(s):  
D. E. BRAGDON ◽  
J.F. A. MCMANUS
Keyword(s):  
Alkaline Phosphatase ◽  
Smooth Muscle ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Venom Gland ◽  
Specific Alkaline Phosphatase ◽  
Rattlesnake Venom ◽  
Great Vessels ◽  
Venom Glands ◽  
Thyroid Epithelium

1. Activity of the specific alkaline phosphatase, 5-nucleotidase, is intense in the epithelium and secretion of the rattlesnake venom gland. Non-specific alkaline phosphatase activity is lacking. 2. Thyroid epithelium, the smooth muscle of great vessels, and (inconstantly) smooth muscle of abdominal hollow viscera show greater 5-nucleotidase than nonspecific activity. 3. These findings confirm the specificity of 5-nucleotidase.

Enzyme-Histochemical Determination of Human Bone Growth Activity in Postmortem Material

1973 ◽  
Vol 52 (6) ◽  
pp. 1293-1296 ◽  
Author(s):  
Inger Kjær ◽  
Uwe Prydsoe
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Incubation Medium ◽  
Room Temperature ◽  
Bone Growth ◽  
Autopsy Material ◽  
Growth Activity ◽  
Enzyme Alkaline Phosphatase

Autopsy material from calvarian bones and sutures of two children, was used to determine growth activity by measuring enzyme alkaline Phosphatase activity. In the extremely active periosteum of the 2-day-old boy, differences in growth activity could be demonstrated by reduction of the temperature of the incubation medium from room temperature to 4 C.

Serum Osteocalcin, Total and Bone-Specific Alkaline Phosphatase following Isolated Tibial Shaft Fracture

1996 ◽  
Vol 33 (3) ◽  
pp. 196-200 ◽  
Author(s):  
Shirley A Bowles ◽  
Nazeer Kurdy ◽  
Alison M Davis ◽  
Michael W France ◽  
David R Marsh
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Healing Process ◽  
Shaft Fracture ◽  
Tibial Shaft Fracture ◽  
Tibial Shaft ◽  
Post Injury ◽  
Specific Alkaline Phosphatase ◽  
Bone Specific Alkaline Phosphatase

We report the changes in osteocalcin concentrations and in total and bone specific alkaline phosphatase activity occurring in the twenty week period following tibial shaft fracture in 20 subjects. Bone formation during the healing process is reflected by progressive increases in the concentration of osteocalcin and bone specific alkaline phosphatase after week 5 and the latter correlated with the height and weight of the subject. In the early post injury period, total alkaline phosphatase activity increased whereas that of the bone isoenzyme initially fell, starting to rise again during the second week. After an immediate post injury rise, osteocalcin concentration also decreased, reaching a nadir by week 5. As only three of our subjects demonstrated delayed union, we have not been able to demonstrate that biochemical monitoring of the healing process can provide an indication of prognosis in tibial shaft fracture.

Sign in / Sign up

Export Citation Format

Share Document