Nonglutaraldehyde treated porcine pericardium with good biocompatibility, reduced calcification and improved Anti-coagulation for bioprosthetic heart valve applications

Abstract OBJECTIVES While decellularization has previously significantly improved the durability of bioprosthetic tissue, remnant immunogenicity may yet necessitate masking through crosslinking. To alleviate the fears of reintroducing the risk of calcific degeneration, we investigated the application of rationally designed crosslinking chemistry, capable of abrogating mineralization in isolation, in decellularized tissue. METHODS Bovine and porcine pericardium were decellularized using the standard Triton X/sodium deoxycholate/DNAse/RNAse methodology and thereafter combined incrementally with components of a four-stage high-density dialdehyde-based fixation regimen. Mechanical properties prior to, and calcium levels following, subcutaneous implantation for 6 and 10 weeks in rats were assessed. RESULTS Enhanced four-stage crosslinking, independent of decellularization, or decellularization followed by any of the crosslinking regimens, achieved sustained, near-elimination of tissue calcification. Decellularization additionally resulted in significantly lower tissue stiffness and higher fatigue resistance in all groups compared to their non-decellularized counterparts. CONCLUSIONS The dual approach of combining decellularization with enhanced crosslinking chemistry in xenogeneic pericardial tissue offers much promise in extending bioprosthetic heart valve longevity.

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Prevention of Bioprosthetic Heart Valve Calcification: Strategies and Outcomes

Current Medicinal Chemistry ◽

10.2174/0929867321666131212151216 ◽

2014 ◽

Vol 21 (22) ◽

pp. 2553-2564 ◽

Cited By ~ 9

Author(s):

L.P. Bre ◽

R. McCarthy ◽

W. Wang

Keyword(s):

Heart Valve ◽

Bioprosthetic Heart Valve ◽

Valve Calcification

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Prevention of Bioprosthetic Heart Valve Calcification by Ethanol Preincubation

Circulation ◽

10.1161/01.cir.95.2.479 ◽

1997 ◽

Vol 95 (2) ◽

pp. 479-488 ◽

Cited By ~ 144

Author(s):

Narendra Vyavahare ◽

Danielle Hirsch ◽

Eyal Lerner ◽

Jonathan Z. Baskin ◽

Frederick J. Schoen ◽

...

Keyword(s):

Heart Valve ◽

Bioprosthetic Heart Valve ◽

Valve Calcification

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Development of bioprosthetic heart valve calcification in vitro and in animal models: morphology and composition

Journal of Crystal Growth ◽

10.1016/s0022-0248(99)00277-8 ◽

1999 ◽

Vol 205 (4) ◽

pp. 554-562 ◽

Cited By ~ 17

Author(s):

D Mavrilas ◽

A Apostolaki ◽

J Kapolos ◽

P.G Koutsoukos ◽

M Melachrinou ◽

...

Keyword(s):

Animal Models ◽

Heart Valve ◽

Bioprosthetic Heart Valve ◽

Valve Calcification

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Immune Response in Patients Receiving a Bioprosthetic Heart Valve: Lack of Response with Decellularized Valves

Tissue Engineering Part A ◽

10.1089/ten.tea.2011.0046 ◽

2011 ◽

Vol 17 (19-20) ◽

pp. 2399-2405 ◽

Cited By ~ 51

Author(s):

Oliver Bloch ◽

Pia Golde ◽

Pascal M. Dohmen ◽

Steffen Posner ◽

Wolfgang Konertz ◽

...

Keyword(s):

Immune Response ◽

Heart Valve ◽

Bioprosthetic Heart Valve

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An improved pericardial bioprosthetic heart valve Design and laboratory evaluation

European Journal of Cardio-Thoracic Surgery ◽

10.1016/1010-7940(87)90015-7 ◽

1987 ◽

Vol 1 (2) ◽

pp. 71-79 ◽

Cited By ~ 12

Author(s):

J FISHER ◽

D WHEATLEY

Keyword(s):

Heart Valve ◽

Laboratory Evaluation ◽

Bioprosthetic Heart Valve ◽

Valve Design

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Dialdehyde pectin-crosslinked and hirudin-loaded decellularized porcine pericardium with improved matrix stability, enhanced anti-calcification and anticoagulant for bioprosthetic heart valves

Biomaterials Science ◽

10.1039/d1bm01297e ◽

2021 ◽

Author(s):

Mengyue Hu ◽

Xu Peng ◽

Yang Zhao ◽

Xiaoshuang Yu ◽

Can Cheng ◽

...

Keyword(s):

Heart Valve ◽

Heart Valves ◽

Heart Valve Diseases ◽

Bioprosthetic Heart Valves ◽

Matrix Stability ◽

Transcatheter Valve ◽

Valve Diseases ◽

Porcine Pericardium

To conveniently and effectively cure heart valve diseases or defects, combining with transcatheter valve technology, bioprosthetic heart valves (BHVs) originated from the decellularized porcine pericardium (D-PP) have been broadly used...

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