Selective interactions of spinophilin with the C-terminal domains of the δ- and μ-opioid receptors and G proteins differentially modulate opioid receptor signaling

2012 ◽  
Vol 24 (12) ◽  
pp. 2315-2328 ◽  
Author(s):  
Danai-Dionysia Fourla ◽  
Maria-Pagona Papakonstantinou ◽  
Stavroula-Maria Vrana ◽  
Zafiroula Georgoussi
Keyword(s):  
G Proteins ◽  
Opioid Receptor ◽  
Opioid Receptors ◽  
Receptor Signaling ◽  
Μ Opioid Receptors ◽  
Selective Interactions ◽  
Terminal Domains

Selective interactions between G protein subunits and RGS4 with the C-terminal domains of the μ- and δ-opioid receptors regulate opioid receptor signaling

2006 ◽  
Vol 18 (6) ◽  
pp. 771-782 ◽  
Author(s):  
Zafiroula Georgoussi ◽  
Leonidas Leontiadis ◽  
Georgia Mazarakou ◽  
Manolis Merkouris ◽  
Karren Hyde ◽  
...  
Keyword(s):  
G Protein ◽  
Opioid Receptor ◽  
Opioid Receptors ◽  
Receptor Signaling ◽  
Protein Subunits ◽  
Selective Interactions ◽  
Terminal Domains

scholarly journals Selective Interactions between Helix VIII of the Human μ-Opioid Receptors and the C Terminus of Periplakin Disrupt G Protein Activation

2003 ◽  
Vol 278 (35) ◽  
pp. 33400-33407 ◽  
Author(s):  
Giu-Jie Feng ◽  
Elaine Kellett ◽  
Carol A. Scorer ◽  
Jonathan Wilde ◽  
Julia H. White ◽  
...  
Keyword(s):  
G Protein ◽  
Opioid Receptors ◽  
Protein Activation ◽  
C Terminus ◽  
G Protein Activation ◽  
Μ Opioid Receptors ◽  
Selective Interactions

scholarly journals Tonic GABAA receptor conductance in medial subnucleus of the tractus solitarius neurons is inhibited by activation of μ-opioid receptors

2012 ◽  
Vol 107 (3) ◽  
pp. 1022-1031 ◽  
Author(s):  
Melissa A. Herman ◽  
Richard A. Gillis ◽  
Stefano Vicini ◽  
Kenneth L. Dretchen ◽  
Niaz Sahibzada
Keyword(s):  
Opioid Receptor ◽  
Opioid Receptors ◽  
Receptor Activation ◽  
Membrane Excitability ◽  
Selective Blockade ◽  
Medial Nucleus ◽  
Opioid Receptor Agonist ◽  
Gaba Signaling ◽  
Μ Opioid Receptor ◽  
Μ Opioid Receptors

Our laboratory previously reported that gastric activity is controlled by a robust GABAA receptor-mediated inhibition in the medial nucleus of the tractus solitarius (mNTS) ( Herman et al. 2009 ), and that μ-opioid receptor activation inhibits gastric tone by suppression of this GABA signaling ( Herman et al. 2010 ). These data raised two questions: 1) whether any of this inhibition was due to tonic GABAA receptor-mediated conductance in the mNTS; and 2) whether μ-opioid receptor activation suppressed both tonic and phasic GABA signaling. In whole cell recordings from rat mNTS neurons, application of three GABAA receptor antagonists (gabazine, bicuculline, and picrotoxin) produced a persistent reduction in holding current and decrease in population variance or root mean square (RMS) noise, suggesting a blockade of tonic GABA signaling. Application of gabazine at a lower concentration abolished phasic currents, but had no effect on tonic currents or RMS noise. Application of the δ-subunit preferring agonist gaboxadol (THIP) produced a dose-dependent persistent increase in holding current and RMS noise. Pretreatment with tetrodotoxin prevented the action of gabazine, but had no effect on the THIP-induced current. Membrane excitability was unaffected by the selective blockade of phasic inhibition, but was increased by blockade of both phasic and tonic currents. In contrast, activation of tonic currents decreased membrane excitability. Application of the μ-opioid receptor agonist DAMGO produced a persistent reduction in holding current that was not observed following pretreatment with a GABAA receptor antagonist and was not evident in mice lacking the δ-subunit. These data suggest that mNTS neurons possess a robust tonic inhibition that is mediated by GABAA receptors containing the δ-subunit, that determines membrane excitability, and that is partially regulated by μ-opioid receptors.

Properties of TAN-67, a nonpeptide δ-opioid receptor agonist, at cloned human δ-and μ-opioid receptors

1995 ◽  
Vol 291 (2) ◽  
pp. 129-134 ◽  
Author(s):  
Richard J. Knapp ◽  
Robert Landsman ◽  
Sue Waite ◽  
Ewa Malatynska ◽  
Eva Varga ◽  
...  
Keyword(s):  
Opioid Receptor ◽  
Opioid Receptors ◽  
Receptor Agonist ◽  
Opioid Receptor Agonist ◽  
Δ Opioid Receptor ◽  
Μ Opioid Receptors
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