scholarly journals Campylobacter phages use hypermutable polyG tracts to create phenotypic diversity and evade bacterial resistance

Cell Reports ◽  
2021 ◽  
Vol 35 (10) ◽  
pp. 109214
Author(s):  
Martine C. Holst Sørensen ◽  
Amira Vitt ◽  
Horst Neve ◽  
Matteo Soverini ◽  
Stephen James Ahern ◽  
...  
Keyword(s):  
Bacterial Resistance ◽  
Phenotypic Diversity

scholarly journals Prevalence and correlates of phenazine resistance in culturable bacteria from a dryland wheat field

2021 ◽  
Author(s):  
Elena Perry ◽  
Dianne K. Newman
Keyword(s):  
Bacterial Resistance ◽  
Phenotypic Diversity ◽  
Culturable Bacteria ◽  
Bacterial Strains ◽  
Wheat Field ◽  
Gram Positive Bacteria ◽  
Wheat Rhizosphere ◽  
Redox Active ◽  
Strain Collection

Phenazines are a class of bacterially-produced redox-active natural antibiotics that have demonstrated potential as a sustainable alternative to traditional pesticides for the biocontrol of fungal crop diseases. However, the prevalence of bacterial resistance to agriculturally-relevant phenazines is poorly understood, limiting both the understanding of how these molecules might shape rhizosphere bacterial communities and the ability to perform risk assessment for off-target effects. Here, we describe profiles of susceptibility to the antifungal agent phenazine-1-carboxylic acid (PCA) across more than 100 bacterial strains isolated from a wheat field where PCA producers are indigenous and abundant. We find that Gram-positive bacteria are typically more sensitive to PCA than Gram-negative bacteria, but that there is also significant variability in susceptibility both within and across phyla. Phenazine-resistant strains are more likely to be isolated from the wheat rhizosphere, where PCA producers are also more abundant, compared to bulk soil. Furthermore, PCA toxicity is pH-dependent for most susceptible strains and broadly correlates with PCA reduction rates, suggesting that uptake and redox-cycling are important determinants of phenazine toxicity. Our results shed light on which classes of bacteria are most likely to be susceptible to phenazine toxicity in acidic or neutral soils. In addition, the taxonomic and phenotypic diversity of our strain collection represents a valuable resource for future studies on the role of natural antibiotics in shaping wheat rhizosphere communities.

Isotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome

2019 ◽  
Author(s):  
Patrick R. A. Zanon ◽  
Lisa Lewald ◽  
Stephan M. Hacker
Keyword(s):  
Binding Sites ◽  
Bacterial Resistance ◽  
Mevalonate Pathway ◽  
Rapid Development ◽  
High Reactivity ◽  
Functional Importance ◽  
Essential Proteins ◽  
Covalent Inhibitors ◽  
Druggable Targets ◽  
Covalent Ligands

Rapid development of bacterial resistance has led to an urgent need to find new druggable targets for antibiotics. In this context, residue-specific chemoproteomic approaches enable proteome-wide identification of binding sites for covalent inhibitors. Here, we describe isotopically labeled desthiobiotin azide (isoDTB) tags that are easily synthesized, shorten the chemoproteomic workflow and allow an increased coverage of cysteines in bacterial systems. We quantify 59% of all cysteines in essential proteins in <i>Staphylococcus aureus</i> and discover 88 cysteines with high reactivity, which correlates with functional importance. Furthermore, we identify 268 cysteines that are engaged by covalent ligands. We verify inhibition of HMG-CoA synthase, which will allow addressing the bacterial mevalonate pathway through a new target. Overall, a comprehensive map of the bacterial cysteinome is obtained, which will facilitate the development of antibiotics with novel modes-of-action.

Phenotypic diversity ofCoreopsis leavenworthiiTorr. & Gray (Asteraceae)

2007 ◽  
Vol 8 (1) ◽  
pp. 45-57
Author(s):  
David M Czarnecki ◽  
Jeffrey G Norcini ◽  
Zhanao Deng
Keyword(s):  
Phenotypic Diversity

Phenotypic diversity of sugarcane variety germplasm

2010 ◽  
Vol 18 (1) ◽  
pp. 37 ◽  
Author(s):  
Liu Xinlong ◽  
Cai Qing ◽  
Wu Caiwen ◽  
Ma Li ◽  
Ying Xiongmei ◽  
...  
Keyword(s):  
Phenotypic Diversity ◽  
Sugarcane Variety
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