Phenotypic diversity and molecular characterization of Cronobacter sakazakii isolates from environmental and food sources

2016 ◽  
Author(s):  
Khaled Ibrahim
Keyword(s):  
Molecular Characterization ◽  
Phenotypic Diversity ◽  
Food Sources ◽  
Cronobacter Sakazakii

scholarly journals Molecular characterization of Cronobacter sakazakii isolated from different herbal teas and mixtures in Serbia

Genetika ◽  
2017 ◽  
Vol 49 (3) ◽  
pp. 921-934
Author(s):  
Dragana Josic ◽  
Marija Stojanovic ◽  
Zorica Lepsanovic ◽  
Vera Katic
Keyword(s):  
Molecular Characterization ◽  
Infant Formula ◽  
Powdered Infant Formula ◽  
Cronobacter Sakazakii ◽  
Safety Risks ◽  
High Prevalence ◽  
Sequences Analysis ◽  
Human Infections ◽  
Rrna Sequences

Cronobacter sakazakii is an important cause of human infections that can be serious and even fatal among premature neonates and immunocompromised adults or infants. Because of its high tolerance to osmotic stress, C. sakazakii is frequently isolated from dried foods, such as powdered infant formula and herbal teas. The aim of investigation was detection, identification and molecular characterization of Cronobacter sakazakii isolates from infant formula and various herbal teas collected from Serbian market and tested for import control. C. sakazakii was not detected in any of the 360 analysed samples of powdered infant formula. However, 192 out of 520 samples of herbal teas tested were positive for C. sakazakii (37.1%). The high prevalence was observed in teas for children (51.6%) and in ?baby? teas (44.1%), followed by medicinal teas (38%). The largest one-herb-teas group (221 samples) contained 72 C. sakazakii-positive samples (32.6%) and involved Sennae folium, Althaeae radix, Menthae piperitae folium, Chamomilae flos and Urticae folium teas. Molecular characterization of isolated C. sakazakii from different herbal teas by rep-PCR, RAPD and 16S rRNA sequences analysis showed the high similarity to C. sakazakii NCTC 8155. Knowing this strain as one of the most pathogenic clinical strains, our results raise concern about the safety risks these foods pose to immunocompromised and healthy consumers, especially for babies and children.

scholarly journals Agronomic, physiological and molecular characterization of spring wheat (Triticum aestivum L.) accessions for drought tolerance

2020 ◽  
Vol 49 (1) ◽  
pp. 29-38
Author(s):  
Muhammad Qadir Ahmad ◽  
Muhammad Farooq Naseer ◽  
Abdul Qayyum ◽  
Sami Ul-Allah ◽  
Waqas Malik ◽  
...  
Keyword(s):  
Drought Stress ◽  
Molecular Characterization ◽  
Genetic Association ◽  
Spring Wheat ◽  
Phenotypic Diversity ◽  
Grain Filling ◽  
Triticum Aestivum L ◽  
Kernel Weight ◽  
Chlorophyll Contents

A comprehensive study was conducted to investigate (i) the effect of drought stress on genetic association among various yield traits and (ii) molecular and phenotypic diversity in the selected spring wheat accessions. A panel of 24 spring wheat accessions was evaluated under normal irrigation and drought stress. Data were collected for chlorophyll contents, canopy temperature, days to heading, grain filling period, relative water content, plant height, peduncle length, number of kernels per spike, 1000-kernel weight and grain yield. Analysis of variance depicted significant differences for genotypes (G), effect of treatment (T) and interaction between T and G. Correlation analysis revealed that genetic association among various traits was stronger in normal treatment than water stressed. The Biplot analysis grouped the genotypes into tolerant and susceptible groups. Molecular characterization of tolerant and susceptible genotypes was done with 14 ISSR markers. ISSR primers revealed a mean of 0.63 genetic similarities among genotypes. Polymorphism information content (PIC) values varied from 0.24 to 0.49. The genetic diversity in selected germplasm can be used to develop drought tolerant lines considering the changing pattern of traits association under drought stress.

scholarly journals Detection and Molecular Characterization of <i>Cronobacter sakazakii</i> Isolated from Powdered Infant Formula (PIF) from North Central Region, Nigeria

2020 ◽  
Vol 10 (07) ◽  
pp. 307-317
Author(s):  
Simon I. Enem ◽  
Chidozie O. Ogbu ◽  
Chinwe E. Okoli ◽  
Ene Godwin ◽  
Gabriel K. Omeiza ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Infant Formula ◽  
Central Region ◽  
Powdered Infant Formula ◽  
North Central Region ◽  
Cronobacter Sakazakii ◽  
North Central

Genetic characterization of sunflower breeding resources from Argentina: assessing diversity in key open-pollinated and composite populations

2013 ◽  
Vol 11 (3) ◽  
pp. 238-249 ◽  
Author(s):  
M. V. Moreno ◽  
V. Nishinakamasu ◽  
M. A. Loray ◽  
D. Alvarez ◽  
J. Gieco ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Phenotypic Diversity ◽  
Gene Diversity ◽  
Germplasm Collection ◽  
Cost Effective ◽  
Germplasm Collections ◽  
Efficient Management ◽  
Breeding Programmes ◽  
Average Gene

Open-pollinated (OPs) and composite populations (CPs) represent a valuable resource for sunflower breeding programmes. However, little is known about the levels and distribution of genetic variation within each of these populations. In this study, quantitative and qualitative traits along with molecular markers were used to evaluate 14 populations from the Instituto Nacional de Tecnología Agropecuaria (INTA) sunflower germplasm collection. These populations were chosen to represent historically important accessions that still play a central role within the INTA sunflower breeding programme due to their extensive variation in diverse agronomically important traits. Nine quantitative and eight qualitative agro-morphological descriptors were recorded and compared with those of a larger set of accessions representative of the phenotypic diversity of the sunflower collection. Molecular characterization was conducted on a total of 311 individuals using 16 microsatellite markers. Overall, the average gene diversity was 0.56 and the average number of alleles per locus was 6.25. No statistically significant differences in genetic diversity were detected between the OPs and CPs. Global estimates ofFSTrevealed very high levels of differentiation among accessions (FST= 0.413,P< 0.05). Population structure analyses were consistent with the observed levels of differentiation and identified two major groups. The results of this work show that high global diversity is preserved within the accessions analysed here. Additionally, this study provides a set of reliable and discriminant markers for the cost-effective molecular characterization of sunflower accessions, along with the guidelines for the delineation of sampling strategies for OPs and CPs, thus aiding the efficient management and exploitation of sunflower germplasm collections.

scholarly journals Molecular characterization of Campylobacter spp. isolated from poultry faeces and carcasses in Poland

2011 ◽  
Vol 80 (1) ◽  
pp. 19-27 ◽  
Author(s):  
Kinga Wieczorek ◽  
Jacek Osek
Keyword(s):  
Molecular Characterization ◽  
Profile Analysis ◽  
Phenotypic Diversity ◽  
Restriction Analysis ◽  
Virulence Gene ◽  
Poor Correlation ◽  
Molecular Technique ◽  
Gene Markers ◽  
Virulence Markers

Campylobacterinfection is one of the most common enteric human diseases world-wide but the mechanism ofCampylobacterpathogenicity has not been exactly explained yet. One of the main reasons is genotypic, hence phenotypic diversity of the bacterial isolates. The aim of the present study was to perform a molecular characterization of randomly selectedC.jejuniandC. colistrains isolated from poultry faeces and carcasses in Poland. Several virulence gene markers were identified by polymerase chain reaction (PCR). Furthermore, genetic typing has also been used by the macrorestriction profiling with pulsed-field gel electrophoresis (PFGE). The results of the present study showed that all analyzed isolates ofC. jejuni(n = 24) andC. coli(n = 24) contained theflaAandcadFsequences. On the other hand, thevirB11gene was present only in 6 of 48 (12.5%) of the analyzed isolates, whereas most of the strains contained thecdtgenes. Other virulence geneiamlinked toCampylobacterinvasiveness was present in 34 of 48 (72.9%) strains. The restriction analysis of the whole genome digested withSmaI produced three main clonal groups designed as I, II (with two subgroups IIa and IIb), and III obtained by the comparison of macrorestriction profiling patterns. The results showed a poor correlation betweenCampylobacterprofiles generated by a clonal molecular technique and the presence of virulence markers. Therefore, PCR detection ofCampylobactervirulence markers can be utilized as a simple and rapid tool to discriminate stains recovered from different sources, especially when used in conjunction with the PFGE profile analysis as a complex strategy. These kinds of analyses had not been previously carried out in Poland and these results may generate more knowledge regarding the genetic diversity and molecular relationship ofCampylobacter.

Prevalence, identification and molecular characterization of Cronobacter sakazakii isolated from retail meat products

Food Control ◽  
2015 ◽  
Vol 53 ◽  
pp. 206-211 ◽  
Author(s):  
Mahmoud Ahmed Mohammed ◽  
Khalid Ibrahim Sallam ◽  
Tomohiro Tamura
Keyword(s):  
Molecular Characterization ◽  
Meat Products ◽  
Cronobacter Sakazakii ◽  
Retail Meat

1446: Molecular Characterization of Cyclooxygenase-2 in a Stimulated Prostate Stroma Cells

2006 ◽  
Vol 175 (4S) ◽  
pp. 467-467
Author(s):  
Victor K. Lin ◽  
Shih-Ya Wang ◽  
Claus G. Roehrbom
Keyword(s):  
Molecular Characterization ◽  
Cyclooxygenase 2 ◽  
Prostate Stroma
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