The results of laboratory experiments on nitroglycerine toxicity detected by histological and cytological indices are presented, using Danio rerio as a case study. For the first time, there are shown the changes in liver and kidneys, both at tissular and cellular levels, induced by administered concentrations of nitroglycerine. The results obtained appear to be important for water bodies’ preservation and elaboration of MPC standards. The intake of nitroglycerine into water bodies is due, mainly, to discharge from pharmaceutical enterprises, demolition works, and outflow from explosives storehouses. Fish turned out to be sensitive to nitroglycerine as indicated by histological and hematological indices. Maximum permissible concentration, MPC, for Danio rerio is determined to be equal to 0.5 mg/l.