Variations of N concentrations and microbial community in the start-up of anammox using anaerobic heterotrophic sludge: Influence of a long reaction-phase time and comparison of the efficiencies of attached-versus suspended-growth cultures

Abstract The effects of FeS on nitrogen removal performance and microbial community of anammox process were studied. During the start-up period, the removal efficiencies of nitrite and total nitrogen were significantly improved by FeS. The addition of FeS increased the content of iron ions in the reactor and promoted the synthesis of heme c, which was involved in the formation of various enzymes. Compared with the control, the abundance of anammox bacteria in the FeS reactor was increased by 29%, and the expression level of the nirS gene (encoding cd1 type nitrite reductase containing heme) was nearly doubled. The content of nitrite reductase (ammonia-forming) in the community was increased by 26.4%. The difference in functional bacteria and enzyme contents in the microbial community resulted in a difference in nitrogen removal rate (NRR) between the two reactors. High-throughput results indicated that FeS increased the richness and diversity of microbial community and enhanced the metabolic function of the microbial community. The addition of FeS did not change the dominant position of Ca. Kuenenia in both reactors. But the relative abundance of heterotrophic denitrifying bacteria was reduced with FeS, which may be related to the inhibition effect of S2− produced by FeS.

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Effect of seed sludge microbial community and activity on the performance of anaerobic reactors during the start-up period

World Journal of Microbiology and Biotechnology ◽

10.1007/s11274-011-0857-5 ◽

2011 ◽

Vol 28 (2) ◽

pp. 637-647 ◽

Cited By ~ 14

Author(s):

N. A. Oz ◽

O. Ince ◽

G. Turker ◽

B. K. Ince

Keyword(s):

Microbial Community ◽

Anaerobic Reactors ◽

Start Up ◽

Seed Sludge

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Performance and Microbial Characteristics of a Hybrid Biological Reactor Treating Industrial Wastewater

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.485.438 ◽

2012 ◽

Vol 485 ◽

pp. 438-441

Author(s):

Chao Li ◽

Xian Li Li ◽

Min Ji ◽

Yu Kun Ma

Keyword(s):

Microbial Community ◽

Industrial Wastewater ◽

Denitrifying Bacteria ◽

Nitrifying Bacteria ◽

Cloning And Sequencing ◽

Attached Growth ◽

Microbial Characteristics ◽

Suspended Growth ◽

Polymerase Chain

To reveal the succession procedure of microbial community in hybrid biological reactor (HBR), the molecular biological techniques of polymerase chain reaction-denaturing gel gradient electrophoresis (PCR-DGGE), cloning and sequencing and ﬂuorescence in situ hybridization (FISH) were applied. PCR-DGGE results showed that the microbial community accumulated in both suspended-growth and attached-growth biomasses. Proteobacteria was found to be the dominant genera of bacteria in the sludge. Denitrifying bacteria was found accumulated in the biofilms. FISH results showed that there were more nitrifying bacteria in the suspended-growth biomass than the attached-growth biomass, but less denitrifying bacteria in the former biomass.

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