Kinetics of pore formation in stearoyl-oleoyl-phosphatidylcholine vesicles by pH sensitive cell penetrating peptide GALA

2021 ◽  
pp. 105139
Author(s):  
Honey Priya James ◽  
Sameer Jadhav
Keyword(s):  
Pore Formation ◽  
Ph Sensitive ◽  
Cell Penetrating Peptide ◽  
Sensitive Cell ◽  
Cell Penetrating ◽  
Kinetics Of

scholarly journals Tumor targeting of a cell penetrating peptide by fusing with a pH-sensitive histidine-glutamate co-oligopeptide

Biomaterials ◽  
2014 ◽  
Vol 35 (13) ◽  
pp. 4082-4087 ◽  
Author(s):  
Likun Fei ◽  
Li-Peng Yap ◽  
Peter S. Conti ◽  
Wei-Chiang Shen ◽  
Jennica L. Zaro
Keyword(s):  
Tumor Targeting ◽  
Ph Sensitive ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
A Cell

scholarly journals Reversible activation of pH-sensitive cell penetrating peptides attached to gold surfaces

2015 ◽  
Vol 51 (2) ◽  
pp. 273-275 ◽  
Author(s):  
Joe E. Baio ◽  
Denise Schach ◽  
Adrian V. Fuchs ◽  
Lars Schmüser ◽  
Nils Billecke ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Cell Penetrating Peptides ◽  
Ph Sensitive ◽  
Sensitive Cell ◽  
Gold Surfaces ◽  
Transition Mechanism ◽  
Coil Transition ◽  
Cell Penetrating ◽  
Covalently Bound

Here, we demonstrate that a fusion protein mimic, designed to achieve a reversible, pH-driven helix-coil transition mechanism, retain its functionality when covalently bound to a surface.

Dual-modified liposomes with a two-photon-sensitive cell penetrating peptide and NGR ligand for siRNA targeting delivery

Biomaterials ◽  
2015 ◽  
Vol 48 ◽  
pp. 84-96 ◽  
Author(s):  
Yang Yang ◽  
YanFang Yang ◽  
XiangYang Xie ◽  
ZhiYuan Wang ◽  
Wei Gong ◽  
...  
Keyword(s):  
Cell Penetrating Peptide ◽  
Sensitive Cell ◽  
Two Photon ◽  
Cell Penetrating ◽  
Targeting Delivery

scholarly journals Potassium channels, megapolarization, and water pore formation are key determinants for cationic cell-penetrating peptide translocation into cells

2020 ◽  
Author(s):  
Evgeniya Trofimenko ◽  
Gianvito Grasso ◽  
Mathieu Heulot ◽  
Nadja Chevalier ◽  
Marco A. Deriu ◽  
...  
Keyword(s):  
Potassium Channels ◽  
Pore Formation ◽  
Transmembrane Potential ◽  
Cellular Membrane ◽  
Cell Penetrating Peptides ◽  
Bioactive Molecules ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating

SummaryCell-penetrating peptides (CPPs) allow intracellular delivery of cargo molecules. CPPs provide efficient methodology to transfer bioactive molecules in cells, in particular in conditions when transcription or translation of cargo-encoding sequences is not desirable or achievable. The mechanisms allowing CPPs to enter cells are ill-defined and controversial. This work identifies potassium channels as key regulators of cationic CPP translocation. Using a CRISPR/Cas9-based screening, we discovered that KCNQ5, KCNN4, and KCNK5 positively modulate CPP cellular direct translocation by reducing transmembrane potential (Vm). Cationic CPPs further decrease the Vm to megapolarization values (about −150 mV) leading to the formation of ∼2 nm-wide water pores used by CPPs to access the cell’s cytoplasm. Pharmacological manipulation to lower transmembrane potential boosted CPPs cellular uptake in zebrafish and mouse models. Besides identifying the first genes that regulate CPP translocation, this work characterizes key mechanistic steps used by CPPs to cross cellular membrane. This opens the ground for pharmacological strategies augmenting the susceptibility of cells to capture CPP-linked cargos in vitro and in vivo.

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