Serum Response Factor Promotes Cigarette Smoke-Induced Proliferation of Rat Pulmonary Artery Smooth Muscle Cells Through Upregulating Cyclin D1 Expression

CHEST Journal ◽  
2017 ◽  
Vol 152 (4) ◽  
pp. A1009
Author(s):  
Ran Wang ◽  
Li Sun ◽  
Gengyun Sun
Keyword(s):  
Smooth Muscle ◽  
Pulmonary Artery ◽  
Smooth Muscle Cells ◽  
Cyclin D1 ◽  
Cigarette Smoke ◽  
Serum Response Factor ◽  
Muscle Cells ◽  
Response Factor ◽  
Pulmonary Artery Smooth Muscle ◽  
Serum Response

Telokin expression in A10 smooth muscle cells requires serum response factor

1997 ◽  
Vol 272 (4) ◽  
pp. C1394-C1404 ◽  
Author(s):  
B. P. Herring ◽  
A. F. Smith
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Transcription Initiation ◽  
Serum Response Factor ◽  
Muscle Cells ◽  
Tata Box ◽  
Luciferase Reporter ◽  
Response Factor ◽  
Specific Expression ◽  
Serum Response

Telokin transcription is initiated from a smooth muscle-specific promoter located in an intron of the smooth muscle myosin light chain kinase gene. We have previously identified a 310-base pair fragment of the promoter that mediates A10 smooth muscle cell-specific expression of telokin. In the current study, telokin-luciferase reporter gene assays in A10 cells and REF52 nonmuscle cells revealed that the promoter region between -81 and +80 contains the regulatory elements required to mediate the in vitro cell specificity of the promoter. Several positive-acting elements, including an E box, myocyte enhancer factor 2 (MEF2)-TATA box, and CArG-serum response element, were identified within this region. Telokin transcription in A10 smooth muscle cells requires all three transcription initiation sites and an AT-rich sequence between -71 and -62 that includes a TATA box. MEF2 interacts with the AT-rich region with low affinity; however, MEF2 binding is not required for transcriptional activity in A10 cells. Binding of serum response factor (SRF) to a CArG element proximal to the TATA sequence is also critical for high levels of transcription in A10 cells. Together these data suggest that an AT-rich motif, acting in concert with SRF and an unusual transcription initiation mechanism, is required for the cell-specific expression of the telokin promoter in A10 smooth muscle cells.

miRNA-181a/b Regulates Phenotypes of Vessel Smooth Muscle Cells Through Serum Response Factor

2017 ◽  
Vol 36 (2) ◽  
pp. 127-135 ◽  
Author(s):  
Xiaoxing Wei ◽  
Xue Hou ◽  
Jianhua Li ◽  
Yongnian Liu
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Serum Response Factor ◽  
Muscle Cells ◽  
Response Factor ◽  
Serum Response

scholarly journals Loss of serum response factor induces microRNA-mediated apoptosis in intestinal smooth muscle cells

2015 ◽  
Vol 6 (12) ◽  
pp. e2011-e2011 ◽  
Author(s):  
C Park ◽  
M Y Lee ◽  
O J Slivano ◽  
P J Park ◽  
S Ha ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Serum Response Factor ◽  
Muscle Cells ◽  
Intestinal Smooth Muscle ◽  
Response Factor ◽  
Serum Response

Testosterone Rescues the De-Differentiation of Smooth Muscle Cells Through Serum Response Factor/Myocardin

2017 ◽  
Vol 232 (10) ◽  
pp. 2806-2817 ◽  
Author(s):  
Carolina Leimgruber ◽  
Amado A. Quintar ◽  
Nahuel Peinetti ◽  
María V. Scalerandi ◽  
Juan P. Nicola ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Serum Response Factor ◽  
Muscle Cells ◽  
Response Factor ◽  
Serum Response
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