Gas chromatographic–mass spectrometric fragmentation study of phytoestrogens as their trimethylsilyl derivatives: Identification in soy milk and wastewater samples

2009 ◽  
Vol 1216 (32) ◽  
pp. 6024-6032 ◽  
Author(s):  
Imma Ferrer ◽  
Larry B. Barber ◽  
E. Michael Thurman
Keyword(s):  
Mass Spectrometric ◽  
Soy Milk ◽  
Mass Spectrometric Fragmentation ◽  
Trimethylsilyl Derivatives ◽  
Wastewater Samples ◽  
Chromatographic Mass

Gaschromatographisch-massenspektrometrische Analyse neutraler und saurer Oligosaccharide der Frauenmilch als Trimethylsilyläther / Gas Chromatographic Mass Spectrometric Analysis of Neutral and Acid Oligosaccharides from Human Milk as Their Trimethylsilyl Derivatives

1975 ◽  
Vol 30 (7-8) ◽  
pp. 451-459 ◽  
Author(s):  
Hubertus von Nicolai ◽  
Heinz Egge ◽  
Fritz Zilliken
Keyword(s):  
Human Milk ◽  
Mass Spectra ◽  
Mass Spectrometric ◽  
Spectrometric Analysis ◽  
Human Milk Oligosaccharides ◽  
Mass Spectrometric Fragmentation ◽  
Trimethylsilyl Derivatives ◽  
Gas Chromatographic Separation ◽  
Oligosaccharide Structures ◽  
Chromatographic Mass

Abstract Gas chromatography and the 20 eV mass spectra of the human milk oligosaccharides fucosido-galactose, fucosido-lactose, di-fucosido-lactose, 3'-N-acetylneuraminyl-lactose, 6'-N-acetylneuraminyl-lactose and of N-acetyllactosamine as pertrimethylsilyl (TMS) ethers are described. The gas chromatographic separation of the L-fucose containing oligosaccharides was performed on Silicone SE 30. The sialic acid containing sugars were separated on DEXSIL 300. The correlations between oligosaccharide structures and mass spectrometric fragmentation pat­ terns are discussed.

Gas-Chromatographic/Mass-Spectrometric Identification and Quantitation of Tetronic and Deoxytetronic Acids in Urine from Normal Adults and Neonates

1975 ◽  
Vol 21 (13) ◽  
pp. 1892-1898 ◽  
Author(s):  
John A Thompson ◽  
Sanford P Markey ◽  
Paul V Fennessey
Keyword(s):  
Mass Spectrometric ◽  
Response Factors ◽  
Computer Determination ◽  
Mass Spectrometric Identification ◽  
Computer Analyses ◽  
Peak Areas ◽  
Trimethylsilyl Derivatives ◽  
Trace Constituents ◽  
Chromatographic Mass

Abstract Gas chromatographic/mass spectrometric/computer analyses of trimethylsilyl derivatives of urine extracts, prepared by an anion-exchange procedure, reveal the presence of several tetronic and deoxytetronic acids. Identifications were confirmed by comparing gas-chromatographic methylene unit values and mass spectrometric characteristics with those of chemically synthesized samples. In addition, several 1,4-lactones corresponding to the acids were detected in small amounts. The acids were measured in groups of adults and neonates by gas chromatographic/computer determination of peak areas and the use of calculated response factors. In urines from adults, 4-deoxythreonic, threonic, and erythronic acids were present in relatively large amounts. 4-Deoxyerythronic and 2-deoxytetronic acids were present in small to moderate amounts; 3-deoxytetronic and 2-methylglyceric acids were only trace constituents. These latter two acids, in addition to 4-deoxyerythronic, were also trace constituents of several urines from neonates but could not be detected in others. Expressed in terms of creatinine excretion, both groups excreted similar amounts of 4-deoxythreonic acid and neonates excreted significantly more 2-deoxytetronic, erythronic, and threonic acids.

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