On the minimization of the band-broadening contributions of a modern, very high pressure liquid chromatograph

2011 ◽  
Vol 1218 (29) ◽  
pp. 4632-4648 ◽  
Author(s):  
Fabrice Gritti ◽  
Georges Guiochon
Keyword(s):  
High Pressure ◽  
Band Broadening ◽  
Liquid Chromatograph ◽  
High Pressure Liquid Chromatograph ◽  
Very High

Switching Valve Designed For High Pressure Liquid Chromatographic Fraction Collection

1979 ◽  
Vol 62 (6) ◽  
pp. 1355-1357
Author(s):  
William O Landen
Keyword(s):  
High Pressure ◽  
Oil Removal ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatograph ◽  
Switching Valve ◽  
Gel Permeation ◽  
In Series ◽  
High Pressure Liquid Chromatograph ◽  
Pass Through ◽  
Liquid Chromatographic

Abstract The use of a collection valve specifically designed for high pressure liquid chromatography is described. Application of the valve to high pressure gel permeation chromatographic (GPC) separation of oil from the vitamin A active fraction of margarine resulted in efficient oil removal after one pass through 2 μStyragel (100A) columns connected in series. Using the normal collection mode of the high pressure liquid chromatograph, 2 passes through the GPC columns were required to adequately resolve the fractions.

Sample Preparation of Carbadox, Furazolidone, Nitrofurazone, and Ethopabate in Medicated Feeds for High Pressure Liquid Chromatography

1980 ◽  
Vol 63 (5) ◽  
pp. 981-984
Author(s):  
Virginia A Thorpe
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Sample Preparation ◽  
Room Temperature ◽  
Quantitative Measurement ◽  
Liquid Chromatograph ◽  
High Pressure Liquid Chromatograph ◽  
Medicated Feeds ◽  
Colorimetric Methods ◽  
Good Agreement

Abstract Medicated feeds (pelleted or mash) containing guarantees of carbadox, furazolidone, nitrofurazone, and ethopabate are pretreated with water, extracted with 95% dimethylformamide overnight at room temperature, cleaned up on a column of alumina, and injected into a high pressure liquid chromatograph for quantitative measurement. Carbadox, nitrofurazone, and furazolidone can be separated; chromatograms show excellent baseline resolution, and results are in good agreement with colorimetric methods. The same extraction and cleanup can be used to improve colorimetric methods for furazolidone and nitrofurazone.

High Pressure Liquid Chromatographic Determination of Xanthomegnin in Corn

1978 ◽  
Vol 61 (3) ◽  
pp. 590-592
Author(s):  
Michael E Stack ◽  
Nathan L Brown ◽  
Robert M Eppley
Keyword(s):  
High Pressure ◽  
Silica Gel ◽  
Chromatographic Determination ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatograph ◽  
High Pressure Liquid Chromatograph ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Layer Chromatography

Abstract A method is described for the detection and quantitative analysis of xanthomegnin in corn samples. Initial extraction with CHCI3 in the presence of 0.5M H3PO4 is followed by additional purification using silica gel column chromatography. A high pressure liquid chromatograph equipped with a microparticle silica gel column and a 405 nm absorbance detector is used for detection and quantitation of the xanthomegnin. The identity of xanthomegnin is confirmed by thin layer chromatography on silica gel plates developed with benzenemethanol- acetic acid (90-(-5+5). The recovery of xanthomegnin added to corn samples at levels of 0.75–9.6 mg/kg averaged 41% with a coefficient of variation of 25%.

Improved Method for High Pressure Liquid Chromatographic Determination of Chlorophacinone in Mouse Tissue

1982 ◽  
Vol 65 (6) ◽  
pp. 1299-1301
Author(s):  
Joseph B Addison
Keyword(s):  
High Pressure ◽  
Chromatographic Determination ◽  
Peak Height ◽  
Mouse Tissue ◽  
Height Measurement ◽  
Liquid Chromatograph ◽  
Order Of Magnitude ◽  
High Pressure Liquid Chromatograph ◽  
Liquid Chromatographic

Abstract A simplified method is described for the determination of chlorophacinone, 2-[(p-chlorophenyl)- phenylacetyl]-l,3-indandione, in homogenized mice. Chlorophacinone is extracted with acetonitrile. After Florisil cleanup, the extract is injected into a high pressure liquid chromatograph for reverse phase chromatography on a polar Lichrosorb NH2 (10 μm) column, with a mobile phase of acetonitrile-water (80 + 20). An injection containing 70 ng chlorophacinone produces 1/2 scale peaks at 254 nm with a full scale absorbance of 0.1 unit, an order of magnitude improvement over the sensitivity reported earlier with a 280 nm detector. Six homogenized mice samples and six spiked homogenized mice samples were quantitatively analyzed for trace levels of chlorophacinone by this method. Recoveries from spiked samples, as determined by peak height measurement, were >95%. Mean retention time for the chlorophacinone peaks in all samples was 6.05 ± 0.05 min. Chlorophacinone levels determined in homogenized whole mouse samples ranged from 0 to 63 ppm.

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