Thin-layer chromatography combined with diode laser thermal vaporization inductively coupled plasma mass spectrometry for the determination of selenomethionine and selenocysteine in algae and yeast

Vidangatandulaadi Choorna is a polyherbal formulation consisting of seven ingredients. Trivrith (Operculina turpethum (Linn.) Silva Manso) is the chief ingredient responsible for the purgative action of the formulation. This yoga is mentioned in Kalpasthana of Ashtangahridaya, intended for virechana (Purgation). It is useful in Kapha-vatha disorders. Even though many kinds of research have been done to identify the physicochemical constituents of individual drugs in the formulation, no studies were done to identify the physicochemical properties of the formulation. This analysis helps in understanding the mechanism for different pharmacological actions of the formulation. Hence, Physico-chemical study of Vidangatandulaadi Choorna along with high-performance thin-layer chromatography (HPTLC) fingerprinting is done to fix the standards. All the drugs included in the formulation is identified by the botanist and is prepared according to Standards mentioned for the preparation of Choorna mentioned in Ayurvedic Pharmacopoeia of India. The formulation is least encountered, but it has shown its significant action in Dyslipidemia in folklore practices. As there are no Standards mentioned for this formulation, the result observed in the present study may be considered suitable. The data obtained from Physicochemical investigation, high-performance thin-layer chromatography profile and ICP-MS (Inductively Coupled Plasma - Mass Spectrometry) could be used as the standards for the present formulation under study.

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Diode Laser Thermal Vaporization Inductively Coupled Plasma Mass Spectrometry

Analytical Chemistry ◽

10.1021/ac202884m ◽

2012 ◽

Vol 84 (5) ◽

pp. 2268-2274 ◽

Cited By ~ 14

Author(s):

Pavla Foltynová ◽

Viktor Kanický ◽

Jan Preisler

Keyword(s):

Mass Spectrometry ◽

Diode Laser ◽

Inductively Coupled Plasma ◽

Inductively Coupled ◽

Thermal Vaporization ◽

Plasma Mass Spectrometry

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Application of Spectroscopic and Chromatographic Methods for Chemical Characterization of an Ayurvedic Herbo-Mineral Preparation: Maha Yograja Guggulu

Journal of Evidence-Based Integrative Medicine ◽

10.1177/2156587217751761 ◽

2018 ◽

Vol 23 ◽

pp. 215658721775176

Author(s):

Arjun Singh ◽

Sarada Ota ◽

Narayan Srikanth ◽

Ruknuddin Galib ◽

Sreedhar Bojja ◽

...

Keyword(s):

Thin Layer ◽

Thin Layer Chromatography ◽

Inductively Coupled Plasma ◽

High Performance ◽

Chemical Characterization ◽

X Ray Diffraction ◽

X Ray ◽

Inductively Coupled ◽

Plasma Mass Spectrometry ◽

Layer Chromatography

Rasa Shastra is an exclusive branch of ayurveda that uses processed metals and minerals in various combinations. Though the formulations are time tested, safety and quality concerns are being raised since the past two decades. In view of this, it becomes mandatory to generate quality control profiles of such formulations by following available parameters. Considering this, we attempted to develop standard manufacturing procedures of Maha Yogaraja Guggulu and generate preliminary physicochemical profiles using inductively coupled plasma mass spectrometry, X-ray diffraction, and high-performance thin-layer chromatography. The results from high-performance thin-layer chromatography revealed presence of organic constituents from plant material. X-ray diffraction indicated that the prepared drug contained cinnabar (mercury sulfide; Rasa sindhura), cassiterite (tin oxide; Vanga bhasma), litharge (lead oxide; Naga bhasma), and iron dioxide and magnetite (di-iron oxide; Loha and Mandura bhasma). The observations of the present study are preliminary and first of its kind that may be considered as baseline data for future studies.

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