scholarly journals Separation of virus-like particles and extracellular vesicles by flow-through and heparin affinity chromatography

2019 ◽  
Vol 1588 ◽  
pp. 77-84 ◽  
Author(s):  
Katrin Reiter ◽  
Patricia Pereira Aguilar ◽  
Viktoria Wetter ◽  
Petra Steppert ◽  
Andres Tover ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Extracellular Vesicles ◽  
Virus Like Particles ◽  
Flow Through ◽  
Heparin Affinity

Simulation of mono-PEGylated lysozyme separation in heparin affinity chromatography using a general rate model

2017 ◽  
Vol 93 (7) ◽  
pp. 1980-1987 ◽  
Author(s):  
Luis Alberto Mejía-Manzano ◽  
Gabriela Sandoval ◽  
M Elena Lienqueo ◽  
Pablo Moisset ◽  
Marco Rito-Palomares ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Rate Model ◽  
Heparin Affinity

A novel purification strategy for retrovirus gene therapy vectors using heparin affinity chromatography

2005 ◽  
Vol 90 (4) ◽  
pp. 391-404 ◽  
Author(s):  
Mar�a de las Mercedes Segura ◽  
Amine Kamen ◽  
Pierre Trudel ◽  
Alain Garnier
Keyword(s):  
Gene Therapy ◽  
Affinity Chromatography ◽  
Gene Therapy Vectors ◽  
Heparin Affinity

Purification of Retrovirus Particles Using Heparin Affinity Chromatography

2008 ◽  
pp. 1-11 ◽  
Author(s):  
María Mercedes Segura ◽  
Amine Kamen ◽  
Alain Garnier
Keyword(s):  
Affinity Chromatography ◽  
Heparin Affinity

Centrifugal system for affinity chromatography with eluate monitoring.

1976 ◽  
Vol 22 (9) ◽  
pp. 1493-1496 ◽  
Author(s):  
S E Shumate ◽  
C D Scott
Keyword(s):  
Affinity Chromatography ◽  
Serum Proteins ◽  
Base Plate ◽  
Rotor Design ◽  
Chromatographic Columns ◽  
Human Immunoglobulins ◽  
Alumina Particles ◽  
Flow Through ◽  
Photometric Monitoring ◽  
Aluminum Base

Abstract A prototype centrifugal system has been developed that permits parallel photometric monitoring of eluate streams from a radial array of chromatographic columns. The modular rotor design consists of a discoidal center insert for eluent and sample apportionment, the chromatographic columns, and flow-through cuvetts-all mounted on an aluminum base plate. A common sample is introduced simultaneously to each column; a single eluent stream is used for all columns. The goal is to assay simultaneously for eight to 16 serum proteins separated by affinity chromatography from a single sample. The system is here exemplified by the use of immunosorbents (consisting of antibodies to human immunoglobulins covalently bound to alumina particles) to allow simultaneous determinations of IgG AND IgM from a single human serum sample in less than 16 min.

Heparin-Affinity of Antithrombin III in a Family With Congenital Antithrombin III Deficiency

1979 ◽  
Author(s):  
G. Sas ◽  
D. Bánhegyi ◽  
I. Petö
Keyword(s):  
Affinity Chromatography ◽  
Antithrombin Iii ◽  
Normal Person ◽  
Agarose Gel ◽  
Functional Methods ◽  
At Iii ◽  
Antithrombin Iii Deficiency ◽  
Heparin Affinity

We found a new thrombophilic family with antithrombin III/ AT-III / deficiency. In the members of this family both immunologic and functional methods revealed similarly decreased levels of AT-III. Gelfiltration displayed identical size of AT-III molecule of patient and normal alike. On the basis of tnese findings we assumed that in this family normal AT-III is produced Dut only in diminished quantity. Experiments on the heparin-affinity of AT-III did not support this assumption. The AT-III of the proposita migrated slower than that of normal person in the heparinized agarose gel. In the course of the heparin-affinity chromatography the AT-III of the proposita could be eluted at lower salt concentrations than normal AT-III.Thus we conclude that even in the case of “true” AT-III deficiency the molecule might have some qualitative deviation from normal.

Optimized purification of mono-PEGylated lysozyme by heparin affinity chromatography using response surface methodology

2017 ◽  
Vol 92 (10) ◽  
pp. 2554-2562 ◽  
Author(s):  
Luis Alberto Mejía-Manzano ◽  
M Elena Lienqueo ◽  
Edgardo J Escalante-Vázquez ◽  
Marco Rito-Palomares ◽  
Juan A Asenjo
Keyword(s):  
Response Surface Methodology ◽  
Affinity Chromatography ◽  
Response Surface ◽  
Heparin Affinity

Separation of mouse crushed muscle extract into distinct mitogenic activities by heparin affinity chromatography

1994 ◽  
Vol 160 (3) ◽  
pp. 563-572 ◽  
Author(s):  
Gang Chen ◽  
Zipora Yablonka-Reuveni ◽  
Lebris S. Quinn ◽  
Roger S. Birnbaum
Keyword(s):  
Affinity Chromatography ◽  
Muscle Extract ◽  
Heparin Affinity
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