Centrifugal system for affinity chromatography with eluate monitoring.

1976 ◽  
Vol 22 (9) ◽  
pp. 1493-1496 ◽  
Author(s):  
S E Shumate ◽  
C D Scott
Keyword(s):  
Affinity Chromatography ◽  
Serum Proteins ◽  
Base Plate ◽  
Rotor Design ◽  
Chromatographic Columns ◽  
Human Immunoglobulins ◽  
Alumina Particles ◽  
Flow Through ◽  
Photometric Monitoring ◽  
Aluminum Base

Abstract A prototype centrifugal system has been developed that permits parallel photometric monitoring of eluate streams from a radial array of chromatographic columns. The modular rotor design consists of a discoidal center insert for eluent and sample apportionment, the chromatographic columns, and flow-through cuvetts-all mounted on an aluminum base plate. A common sample is introduced simultaneously to each column; a single eluent stream is used for all columns. The goal is to assay simultaneously for eight to 16 serum proteins separated by affinity chromatography from a single sample. The system is here exemplified by the use of immunosorbents (consisting of antibodies to human immunoglobulins covalently bound to alumina particles) to allow simultaneous determinations of IgG AND IgM from a single human serum sample in less than 16 min.

scholarly journals Separation of virus-like particles and extracellular vesicles by flow-through and heparin affinity chromatography

2019 ◽  
Vol 1588 ◽  
pp. 77-84 ◽  
Author(s):  
Katrin Reiter ◽  
Patricia Pereira Aguilar ◽  
Viktoria Wetter ◽  
Petra Steppert ◽  
Andres Tover ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Extracellular Vesicles ◽  
Virus Like Particles ◽  
Flow Through ◽  
Heparin Affinity

Human Saliva and Semen: Evidence for Proteins Common to Both Which Do Not Occur in Serum

1981 ◽  
Vol 60 (2) ◽  
pp. 179-184 ◽  
Author(s):  
P. D. Eckersall ◽  
J. A. Beeley
Keyword(s):  
Antibacterial Activity ◽  
Affinity Chromatography ◽  
Human Serum ◽  
Serum Protein ◽  
Serum Proteins ◽  
Rabbit Antiserum ◽  
Human Saliva ◽  
Cross Reactions ◽  
Whole Saliva ◽  
The Cross

1. Rabbit antiserum to human whole saliva cross-reacts with both human serum and semen. After absorption of the antiserum by affinity chromatography on a column of immobilized serum protein, the cross-reactions with serum were eliminated. 2. The absorbed antiserum, however, still cross-reacted with semen thus indicating the presence of proteins with immunological similarity in both saliva and semen, but which did not occur in serum. 3. Some of these proteins clearly showed a reaction of complete immunological identity between saliva and semen. 4. The presence of the non-serum proteins in both saliva and semen might be related to common functions in both such as lubrication or antibacterial activity.

The Use of High Pressure Direct Squeeze Casting for Semi-Solid Processing of Aluminum Base Nano Composites

2012 ◽  
Vol 192-193 ◽  
pp. 72-75 ◽  
Author(s):  
David Weiss ◽  
Michael Black
Keyword(s):  
Aluminium Alloys ◽  
Squeeze Casting ◽  
Full Range ◽  
High Surface ◽  
Nano Composites ◽  
High Surface Tension ◽  
Alumina Particles ◽  
Semi Solid ◽  
Aluminum Base ◽  
Suitable Technique

The production of nano-reinforced aluminium alloys in volume and quality suitable for subsequent shape casting has been problematic. Large specific surface area and high interfacial energy of the particles combined with high surface tension of the aluminium melt makes it difficult to add appreciable numbers of particles to the melt, even when later de-agglomerated by techniques such as ultrasonic cavitation. The objective of this work was to develop a technique to incorporate particles using pressure applied while the alloy was in a semi-solid state. The composites produced could be used as a master alloy to inoculate large batches of metal for subsequent casting using any suitable technique. The results show excellent distribution of 50 nm alumina particles in 2014 material. The procedure appears to have broad applicability to a full range of aluminium alloys and particle reinforcements.

scholarly journals Characterization of Drug Interactions with Serum Proteins by Using High-Performance Affinity Chromatography

2011 ◽  
Vol 12 (4) ◽  
pp. 313-328 ◽  
Author(s):  
David S. Hage ◽  
Jeanethe Anguizola ◽  
Omar Barnaby ◽  
Abby Jackson ◽  
Michelle J. Yoo ◽  
...  
Keyword(s):  
Drug Interactions ◽  
Affinity Chromatography ◽  
High Performance ◽  
Serum Proteins ◽  
High Performance Affinity Chromatography

Design methodology of a highly loaded tandem rotor and its performance analysis under clean and distorted inflows

2021 ◽  
pp. 095440622110160
Author(s):  
Amit Kumar ◽  
AM Pradeep
Keyword(s):  
Experimental Analysis ◽  
Total Pressure ◽  
Pressure Rise ◽  
Pressure Gradients ◽  
Complex Flow ◽  
Angle Variation ◽  
Stall Margin ◽  
Rotor Design ◽  
Flow Through ◽  
Aerodynamic Parameters

Compact and efficient compressor design is one of the key challenges in aero-engine development. The flow through a compressor is exposed to adverse pressure gradients, which limits the maximum allowable flow turning in a compressor blade. Tandem blading is an interesting concept to achieve a higher total pressure rise by augmenting the flow turning angle. Variation in axial overlap and percentage pitch of the forward and aft blade elements largely influences the behavior of the tandem configuration. In the present study, the genetic algorithm is used to optimize the axial overlap and the percentage pitch for the tandem rotor. Results indicate that a lower axial overlap and higher percentage pitch results in optimum performance. The paper presents the parametric study of four tandem configurations with different axial overlaps and percentage pitches. A detailed experimental analysis of the four different tandem configurations is included in this paper. The behavior of the tandem rotor is examined under the clean and radially distorted inflow. Further, a comparison is drawn with a conventional single rotor in terms of aerodynamic parameters such as total pressure rise, axial velocity, and stall margin. The experimental analysis is supplemented by some interesting computational results, which are included to provide some insight into the complex flow field of the tandem rotor. Tandem rotor design is observed to have a higher sensitivity to radial tip inflow distortion. The upstream shift of the aft rotor blade adversely affects the total pressure rise and stall margin of the tandem rotor.

scholarly journals Freezing colloidal suspensions: periodic ice lenses and compaction

2014 ◽  
Vol 758 ◽  
pp. 786-808 ◽  
Author(s):  
Anthony M. Anderson ◽  
M. Grae Worster
Keyword(s):  
Colloidal Suspensions ◽  
Periodic Sequence ◽  
Freezing Rate ◽  
Uniform Temperature ◽  
Alumina Particles ◽  
Flow Through ◽  
Lens Growth ◽  
Kinetics Of ◽  
Fully Coupled ◽  
Horizontal Layers

AbstractRecent directional solidification experiments with aqueous suspensions of alumina particles (Anderson & Worster, Langmuir, vol. 28 (48), 2012, pp. 16512–16523) motivate a model for freezing colloidal suspensions that builds upon a theoretical framework developed by Rempel et al. (J. Fluid Mech., vol. 498, 2004, pp. 227–244) in the context of freezing soils. Ice segregates from the suspension at slow freezing rates into discrete horizontal layers of particle-free ice, known as ice lenses. A portion of the particles is trapped between ice lenses, while the remainder are pushed ahead, forming a layer of fully compacted particles separated from the bulk suspension by a sharp compaction front. By dynamically modelling the compaction front, the growth kinetics of the ice lenses are fully coupled to the viscous flow through the evolving compacted layer. We examine the periodic states that develop at fixed freezing rates in a constant, uniform temperature gradient, and compare the results against experimental observations. Congruent with the experiments, three periodic regimes are identified. At low freezing rates, a regular periodic sequence of ice lenses is obtained; predictions for the compacted layer thickness and ice-lens characteristics as a function of freezing rate are consistent with experiments. At intermediate freezing rates, multiple modes of periodic ice lenses occur with a significantly diminished compacted layer. When the cohesion between the compacted particles is sufficiently strong, a sequence of mode-doubling bifurcations lead to chaos, which may explain the disordered ice lenses observed experimentally. Finally, beyond a critical freezing rate, the regime for sustained ice-lens growth breaks down. This breakdown is consistent with the emergence of a distinct regime of ice segregation found experimentally, which exhibits a periodic, banded structure that is qualitatively distinct from ice lenses.

Affinity chromatography of arginase with continuous detection of enzyme using urease membrane electrode

1989 ◽  
Vol 54 (10) ◽  
pp. 2814-2822 ◽  
Author(s):  
Lumír Macholán ◽  
Bohdana Hlavatá
Keyword(s):  
Response Time ◽  
Affinity Chromatography ◽  
Rapid Method ◽  
Reaction Layer ◽  
Liver Extract ◽  
Membrane Electrode ◽  
Affinity Adsorbent ◽  
Enzyme Sequence ◽  
Potentiometric Measurement ◽  
Flow Through

A rapid method is described of potentiometric measurement of liver arginase during its purification on a column of affinity adsorbent prepared by the derivatization of Spheron E-1000. The detection of the enzyme in the flow-through system is effected by an ammonia electrode coated by the reaction layer of glutaraldehyde-crosslinked urease on a polyamide mesh. The method is time- and urease-saving and permits arginase to be obtained for analytical purposes purified 212 times from a liver extract in a 68% yield. By combining the membrane made of crosslinked arginase and urease on a polyamide mesh with a pNH3 electrode we developed a two-enzyme sequence electrode sensitive to L-arginine (10-3 -10-4 mol l-1) showing a response time of 1-3 min and a stability of about 3 weeks.

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