Microchip electrophoresis based multiplexed assay for silver and mercury ions simultaneous detection in complex samples using a stirring bar modified with encoded hairpin probes for specific extraction

A simple double-T type microchip electrophoresis (MCE) platform is presented for the multiplex detection of several quality indicator molecules simultaneously in urine.

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Rapid Multiplexing and Simultaneous Detection of Human Spermatogenetic Failure with a 12 Lane Microchip Electrophoresis System

Biological and Pharmaceutical Bulletin ◽

10.1248/bpb.29.1487 ◽

2006 ◽

Vol 29 (7) ◽

pp. 1487-1489 ◽

Cited By ~ 3

Author(s):

Mohammad Jabasini ◽

Ashraf Abdulazim Ewis ◽

Maged Fouad ◽

Fuquan Dang ◽

Guichen Ping ◽

...

Keyword(s):

Simultaneous Detection ◽

Microchip Electrophoresis ◽

Electrophoresis System

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Simultaneous Detection and Removal of Mercury Ions in Aqueous Solution with Fluorescent Conjugated Polymer-Based Sensor Ensemble

Macromolecular Rapid Communications ◽

10.1002/marc.201100255 ◽

2011 ◽

Vol 32 (14) ◽

pp. 1061-1065 ◽

Cited By ~ 29

Author(s):

Na Young Kwon ◽

Daigeun Kim ◽

Ji Hye Son ◽

Geun Seok Jang ◽

Jung Hyo Lee ◽

...

Keyword(s):

Aqueous Solution ◽

Conjugated Polymer ◽

Simultaneous Detection ◽

Mercury Ions

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Digital Detection of Nanoparticles: Viral Diagnostics and Multiplexed Protein and Nucleic Acid Assays

MRS Proceedings ◽

10.1557/opl.2015.4 ◽

2015 ◽

Vol 1720 ◽

Cited By ~ 5

Author(s):

M. S. Ünlü

Keyword(s):

Limit Of Detection ◽

Simultaneous Detection ◽

Detection Sensitivity ◽

Optical Antenna ◽

Dna Arrays ◽

Complex Samples ◽

Practical Applications ◽

Nanoparticle Detection ◽

Nucleic Acid Assays ◽

Imaging Sensor

ABSTRACTSynthetic nanoparticles have made significant impact across a broad range of technological applications including optical nanoantennas, ultra-sensitive imaging and sensing, and diagnostics and therapeutics. Natural nanoparticles such as viruses and pollutants are major concerns for human health. High-throughput characterization of nanoparticles in terms of their size and shape is crucial for practical applications of synthetic nanoparticles and highly sensitive pathogen identification. Recently, we have demonstrated Interferometric Reflectance Imaging Sensor (IRIS) with the ability to detect single nanoscale particles [1,2].In single-particle modality of IRIS (SP-IRIS), the interference of light reflected from the sensor surface is modified by the presence of particles producing a distinct signal that reveals the size of the particle. In our approach, the dielectric layered structure acts as an optical antenna optimizing the elastic scattering characteristics of nanoparticles for sensitive detection and analysis. We have demonstrated identification of virus articles in complex samples for various viruses in multiplexed format. Size discrimination of the imaged nanoparticles (virions) allows differentiation between modified viruses having different genome lengths and facilitates a reduction in the counting of non-specifically bound particles to achieve a limit-of-detection (LOD) of 5x103 pfu/mL for the Ebola and Marburg VSV pseudotypes. We have demonstrated the simultaneous detection of multiple viruses in serum or whole blood as well as in samples contaminated with high levels of bacteria [3]. Single nanoparticle detection with IRIS has shown promising results for protein [4] and DNA arrays with attomolar detection sensitivity.

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Simultaneous detection of different bacteria by microchip electrophoresis combined with universal primer-duplex polymerase chain reaction

Journal of Chromatography A ◽

10.1016/j.chroma.2019.460734 ◽

2020 ◽

Vol 1615 ◽

pp. 460734

Author(s):

Feifei Luo ◽

Zhi Li ◽

Ge Dai ◽

Yuqi Lu ◽

Pingang He ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Simultaneous Detection ◽

Microchip Electrophoresis ◽

Chain Reaction ◽

Universal Primer ◽

Polymerase Chain ◽

Duplex Polymerase Chain Reaction

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Gluten Analysis in Processed Foodstuffs by a Multi-Allergens and Grain-Specific UHPLC-MS/MS Method: One Method to Detect Them All

Journal of AOAC International ◽

10.1093/jaoac/102.5.1286 ◽

2019 ◽

Vol 102 (5) ◽

pp. 1286-1302 ◽

Cited By ~ 4

Author(s):

Jean Henrottin ◽

Mélanie Planque ◽

Anne-Catherine Huet ◽

Riccardo Marega ◽

Amandine Lamote ◽

...

Keyword(s):

Simultaneous Detection ◽

Autoimmune Disorder ◽

Food Products ◽

Management Policy ◽

Type I ◽

Complex Samples ◽

Detection And Identification ◽

Heat Treated ◽

Gluten Analysis

Abstract Background: Celiac disease, a complex, long-term autoimmune disorder and gluten intolerance, is estimated to affect from 1 to 5% of the world’s population. Objective: As a consequence, to protect gluten-sensitive consumers, the development of reliable analytical methods allowing the detection of gluten in various food products is needed. Methods: Currently, ELISA is probably the most widespread used methodology. The method based on the R5 antibody has received type I status in Codex Alimentarius. However, the ELISA method suffers from some limitations, especially concerning quantification of nonwheat gluten. As a consequence, the development of another complementary methodology such as LC–tandem MS (MS/MS) is considered to be essential. Furthermore, this method could also be used for the simultaneous detection of gluten with other allergens, which will constitute a great additional benefit for producers of “free-from” food products and/or having a management policy integrated for several allergies and/or intolerances. Results: A multi-allergen and grain-specific ultra-HPLC coupled to MS/MS method allowing the identification and the discrimination of gluten from seven cereals, simultaneously with the detection and identification of 10 allergens in only one analysis, is thus described here. Conclusions: This method can be used for the analysis of a broad range of foodstuff matrices containing wheat and/or its derivatives, including cereals, flours, heat-treated and foodstuffs, but also more complex samples having undergone fermentation processes (such as beers).

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